Development of a Silk-Chitosan Blend Scaffold for Bone Tissue Engineering

Ng, Kian Siang; Wong, XY; Goh, James Cho Hong; Toh, S.L.

doi:10.1007/978-3-540-92841-6_341

Cited by 3 publications

(2 citation statements)

References 15 publications

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“…In this regard, many different bone scaffolds made of polymers have been tested for safe use in the human body. These polymers include poly(lactic‐co‐glycolic acid), alginate–hyaluronic acid, polycaprolactone, cellulose–collagen, silk–chitosan and so on (Pan and Ding, ; Rubert et al ., ; Ng et al ., ).…”

Section: Introductionmentioning

confidence: 97%

Hybrid scaffolds based on PLGA and silk for bone tissue engineering

Sheikh

Moon

et al. 2015

J Tissue Eng Regen Med

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Porous silk scaffolds, which are considered to be natural polymers, cannot be used alone because they have a long degradation rate, which makes it difficult for them to be replaced by the surrounding tissue. Scaffolds composed of synthetic polymers, such as PLGA, have a short degradation rate, lack hydrophilicity and their release of toxic by-products makes them difficult to use. The present investigations aimed to study hybrid scaffolds fabricated from PLGA, silk and hydroxyapatite nanoparticles (Hap NPs) for optimized bone tissue engineering. The results from variable-pressure field emission scanning electron microscopy (VP-FE-SEM), equipped with EDS, confirmed that the fabricated scaffolds had a porous architecture, and the location of each component present in the scaffolds was examined. Contact angle measurements confirmed that the introduction of silk and HAp NPs helped to change the hydrophobic nature of PLGA to hydrophilic, which is the main constraint for PLGA used as a biomaterial. Thermo-gravimetric analysis (TGA) and FT-IR spectroscopy confirmed thermal decomposition and different vibrations caused in functional groups of compounds used to fabricate the scaffolds, which reflected improvement in their mechanical properties. After culturing osteoblasts for 1, 7 and 14 days in the presence of scaffolds, their viability was checked by MTT assay. The fluorescent microscopy results revealed that the introduction of silk and HAp NPs had a favourable impact on the infiltration of osteoblasts. In vivo experiments were conducted by implanting scaffolds in rat calvariae for 4 weeks. Histological examinations and micro-CT scans from these experiments revealed beneficial attributes offered by silk fibroin and HAp NPs to PLGA-based scaffolds for bone induction.

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