Development of a simultaneous analytical method to determine arsenic speciation using HPLC-ICP-MS: Arsenate, arsenite, monomethylarsonic acid, dimethylarsinic acid, dimethyldithioarsinic acid, and dimethylmonothioarsinic acid

Jeong, Seulki; Lee, Hosub; Kim, Youn Tae; Yoon, Hoi Soo

doi:10.1016/j.microc.2017.06.011

Cited by 32 publications

(17 citation statements)

References 38 publications

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“…Both carbonate and phosphate buffer solutions have been used for elution of inorganic and organic arsenic species on anion exchange chromatographic columns, although longer retention time and peak broadening often occurred when carbonate buffer solutions were used . Based on the analytical methods developed in our group , mobile phase consisting of (a) 30 mM (NH 4 ) 2 HPO 4 at pH 5.8 (adjusted with dilute HNO 3 solution), and (b) water was chosen for the elution of arsenic species using a gradient program (Supporting Information ) in the HPLC‐ICP‐MS analysis.…”

Section: Resultsmentioning

confidence: 99%

A method for rapid determination of arsenic species in vegetables using microwave‐assisted extraction followed by detection with HPLC hyphenated to inductively coupled plasma‐mass spectrometry

Jia

Yang

Zhao

et al. 2019

J of Separation Science

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Driven by the significant need for characterization of the chemical speciation of arsenic in food, this work developed a method for rapid determination of four common arsenic species, namely, arsenite, arsenate, monomethyl arsenic acid, and dimethyl arsenic acid, in vegetables using microwave‐assisted extraction, followed by detection with high‐performance liquid chromatography hyphenated to inductively coupled plasma‐mass spectrometry. Initial screening results showed that microwave‐assisted extraction using 1% HNO3 exhibited the highest overall efficiencies for all arsenic species without causing significant degradation of the organic ones. With the aid of response surface methodology, the optimum conditions established for extraction of arsenic species from vegetables were: 500 mg of freeze‐dried vegetable sample, extracted by closed vessel microwave‐assisted extraction using 10 mL of 2% v/v HNO3 at 90°C for 17 min. Application of the method in the analysis of 24 market vegetable samples indicates that the extraction efficiencies for total arsenic species were in the range of 91.4–106%. Arsenite and arsenate were found to be the predominant arsenic species in the vegetables, which suggests that vegetable consumption could be an important route of inorganic arsenic exposure for the population with a heavy vegetable diet in arsenic polluted regions.

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Section: Resultsmentioning

confidence: 99%

A method for rapid determination of arsenic species in vegetables using microwave‐assisted extraction followed by detection with HPLC hyphenated to inductively coupled plasma‐mass spectrometry

Jia

Yang

Zhao

et al. 2019

J of Separation Science

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“…There has been several review articles on the speciation of arsenic in a variety of samples [10][11][12][13][14]. These reviews focus on (1) determination of total content of arsenic and (2) speciation analysis.…”

Section: Determination Of Arsenic and Arsenic Species In Watermentioning

confidence: 99%

“…The total concentration of arsenic in drinking water (mostly traces of arsenic, level of μg L −1 or less) can be detected only by sophisticated analytical techniques as ICP-MS, GF-AAS and HG-AAS [3,14]. For As speciation analysis, well-established methods that involve the coupling of separation techniques, such as HPLC with a sensitive detection system, that is, ICP-MS, are recommended, and they are mostly used [13]. Historically, colorimetric/spectrophotometric methods have been used to determine total arsenic concentration.…”

Section: Determination Of Arsenic and Arsenic Species In Watermentioning

confidence: 99%

“…Analytical methods for determining different arsenic species have become increasingly important due to different toxicity and chemical behavior of various arsenic forms. Methods that involve the coupling of separation techniques, such as IC and HPLC with a sensitive detection system, such as ICP-MS, HG-AFS, HG-AAS and GF-AAS [3,11,13,14,19]. HPLC has been a preferred technique used for separation of arsenic compounds.…”

Section: Sophisticated Coupling Techniquementioning

confidence: 99%

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Arsenic in Water: Determination and Removal

Rajaković¹,

Rajaković-Ognjanović²

2018

Arsenic - Analytical and Toxicological Studies

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Depending on the physical, chemical and biogeochemical processes and condition of the environment, various arsenic species can be present in water. Water soluble arsenic species existing in natural water are inorganic arsenic (iAs) and organic arsenic (oAs) species. All acidic species, according to the chemical equilibrium, have well-recognized molecular and ionic forms in water. The distribution of iAs and oAs species is a function of pH value of water traces of arsenic that are found in groundwater, lakes, rivers and ocean. The WHO provisional guideline value for arsenic in drinking water is 10 μg L −1. The most selective and sensitive methods for determination of total arsenic and its species in water are coupled techniques including chromatography, optical methods and mass spectrometry. Determination of arsenic species is of crucial importance for selection of arsenic removal technology. Best available technologies are based on absorption, precipitation, membrane and hybrid membrane processes. Adsorption is considered to be relatively simple, efficient and low-cost removal technique, especially convenient for application in rural areas. Sorbents for arsenic removal are biological materials, mineral oxides, activated carbons and polymer resins.

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“…4. Analyze m/z of DMMTA V using ESI-MS 11,16 and fragments at m/z 155 in the positive-ion mode or at m/z 153 in the negative-ion mode (Table 1). NOTE: See reference values of m/z (Table 1).…”

Section: Assume the Total As Of Used Dmamentioning

confidence: 99%

Preparation of DMMTAV and DMDTAV Using DMAV for Environmental Applications: Synthesis, Purification, and Confirmation

Lee

Kim

Jeong

et al. 2018

JoVE

Self Cite

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Dimethylated thioarsenicals such as dimethylmonothioarsinic acid (DMMTA) and dimethyldithioarsinic acid (DMDTA), which are produced by the metabolic pathway of dimethylarsinic acid (DMA) thiolation, have been recently found in the environment as well as human organs. DMMTA and DMDTA can be quantified to determine the ecological effects of dimethylated thioarsenicals and their stability in environmental media. The synthesis method for these compounds is unstandardized, making replicating previous studies challenging. Furthermore, there is a lack of information about storage techniques, including storage of compounds without species transformation. Moreover, because only limited information about synthesis methods is available, there may be experimental difficulties in synthesizing standard chemicals and performing quantitative analysis. The protocol presented herein provides a practically modified synthesis method for the dimethylated thioarsenicals, DMMTA and DMDTA, and will help in the quantification of species separation analysis using high performance liquid chromatography in conjunction with inductively coupled plasma mass spectrometry (HPLC-ICP-MS). The experimental steps of this procedure were modified by focusing on the preparation of chemical reagents, filtration methods, and storage.

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Development of a simultaneous analytical method to determine arsenic speciation using HPLC-ICP-MS: Arsenate, arsenite, monomethylarsonic acid, dimethylarsinic acid, dimethyldithioarsinic acid, and dimethylmonothioarsinic acid

Cited by 32 publications

References 38 publications

A method for rapid determination of arsenic species in vegetables using microwave‐assisted extraction followed by detection with HPLC hyphenated to inductively coupled plasma‐mass spectrometry

A method for rapid determination of arsenic species in vegetables using microwave‐assisted extraction followed by detection with HPLC hyphenated to inductively coupled plasma‐mass spectrometry

Arsenic in Water: Determination and Removal

Preparation of DMMTA<sup>V</sup> and DMDTA<sup>V</sup> Using DMA<sup>V</sup> for Environmental Applications: Synthesis, Purification, and Confirmation

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