Development of a Single-nucleotide Polymorphism Marker for the Sw-5b Gene Conferring Disease Resistance to Tomato spotted wilt virus in Tomato

Abstract

Tomato spotted wilt virus (TSWV) causes one of the most destructive viral diseases that threatens global tomato production. Sw-5b was reported as the resistance gene effective against TSWV. The objective of this research was to develop a single-nucleotide polymorphism (SNP) marker to distinguish tomato cultivars resistant to TSWV from susceptible cultivars for marker-assisted breeding. First, we determined genotypes for TSWV resistance in 32 commercial tomato cultivars using the previously reported Sw-5b gene-based marker. Then, DNA sequences of Sw-5b alleles in tomato cultivars showing resistant or susceptible genotypes were analyzed; a single SNP was found to distinguish tomato cultivars resistant to TSWV from susceptible cultivars. Based on the confirmed SNP, a SNP primer pair was designed. Using this new SNP sequence and high-resolution melting analysis, the same 32 tomato cultivars were screened. The results were perfectly correlated with those from screening with the Sw-5b gene-based marker. These results indicate that the SNP maker developed in this study will be useful for better tracking of resistance to TSWV in tomato breeding.

The Identification of the Resistance Levels of Fusarium Oxysporum F. Sp. Radicis - Lycopersici and Tomato Yellow Leaf Curl Viruses in Different Tomato Genotypes With Traditional and Molecular Methods

“…The existence of different TYLCV strains, especially in Asia, revealed that different markers should be tested in these areas (Hutton et al, 2012). Israel and Mild strains of TYLCV are the most prevalent strains globally, and the TY1 and TY3 markers developed against these strains maintain the status of being the most used marker in breeding studies in the world (Lee et al, 2015…”

“…In the present study, the use of molecular markers reduced the number of genotypes from 418 to 7 tomato genotypes (B26, B40, B178, A31, A41, A48 and A66) that were resistant to all three factors (FORL+TY1+TY3), making it easier and more economic to verify these findings with classical tests. Although molecular markers provide a great advantage in terms of speed and cost, classic validation tests should be performed (Lee et al, 2015). This is due to the fact that it is important to perform classical validation tests (smyptomatologic) in determination of MAS-selective resistance, which taking the distances between the developed markers and the gene and human and marker-based errors into consideration (Caro et al, 2015;Scott et al, 2015).…”

Genome-Wide Identification and Functions against Tomato Spotted Wilt Tospovirus of PR-10 in Solanum lycopersicum

“…Forty-five amino acid sequences of PR-10 encoding genes were used to analyze the genome-wide identification of the PR-10 superfamily. M82 and H8 tomato seeds were used in this experiment as a sensitive and resistant material because M82 was included as a TSWV susceptible cultivar, as reported by [44], and H8 was included as a TSWV resistant cultivar, as reported by the Laboratory of Tomato Quality and Stress Tolerance Regulation Mechanism and Genetic Improvement, College of Horticulture, Northwest A and F University, China.…”

Interspecific and Intraspecific Hybrid Rootstocks to Improve Horticultural Traits and Soil-Borne Disease Resistance in Tomato

“…The methods of marker-assisted selection (MAS) were performed based on HRM curve method and judged with resistance or susceptibility instead of trait values [ 35 ]. Then, the experiment evaluated resistance with a marker such as: Fusarium wilt; I2 [ 36 ], Fusarium wilt ; I3 [ 37 ], Verticillium wilt; Ve2 [ 38 ], Fusarium crown and root rot ; J3 [ 39 ], Corky root rot ; py1 [ 40 ], Root-Knot nematode ; Mi23 [ 41 ], Bacterial wilt; Bw6 and Bw12 [ 42 ], Tomato Spotted wilt virus ; TSWV or Sw5 [ 43 ], Tomato mosaic virus ; ToMV or Tm2a (Unpublicized data), Tomato yellow leaf curl virus ; TYLCV or Ty1 [ 44 ], Tomato yellow leaf curl virus ; TYLCV or Ty2 [ 45 ], Late blight ; Ph3 [ 46 ], Gray leaf spot ; Sm-565 [ 47 ] and Leaf mold ; Cf9 [ 48 ].…”