Development of a single-tube one-step RT-LAMP assay to detect the Chikungunya virus genome

López-Jimena, Benjamín; Wehner, Stefanie; Harold, Graham; Bakheit, Mohammed A.; Frischmann, Sieghard; Bekaert, Michaël; Faye, Oumar; Sall, Amadou Alpha; Weidmann, Manfred

doi:10.1371/journal.pntd.0006448

Cited by 45 publications

(34 citation statements)

References 46 publications

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“…More recently, related studies have been reported (Lopez-Jimena et al, 2018 andHayashida et al, 2019). Thus, we compared the sensitivity and specificity of our RT-LAMP system to those of their systems.…”

Section: Discussionmentioning

confidence: 99%

Development of a portable reverse transcription loop‐mediated isothermal amplification system to detect the E1 region of Chikungunya virus in a cost‐effective manner

et al. 2020

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Chikungunya fever is a mosquito‐borne disease cause of persistent arthralgia. The current diagnosis of Chikungunya virus (CHIKV) relies on a conventional reverse transcription polymerase chain reaction assay. Reverse transcription loop‐mediated isothermal amplification (RT‐LAMP) is a rapid and simple tool used for DNA‐based diagnosis of a variety of infectious diseases. In this study, we established an RT‐LAMP system to recognize CHIKV by targeting the envelope protein 1 (E1) gene that could also detect CHIKV at a concentration of 8 PFU without incorrectly detecting other mosquito‐borne viruses. The system also amplified the E1 genome in the serum of CHIKV‐infected mice with high sensitivity and specificity. Moreover, we established a dry RT‐LAMP system that can be transported without a cold chain, which detected the virus genome in CHIKV‐infected patient samples with high accuracy. Thus, the dry RT‐LAMP system has great potential to be applied as a novel CHIKV screening kit in endemic areas.

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Section: Discussionmentioning

confidence: 99%

Development of a portable reverse transcription loop‐mediated isothermal amplification system to detect the E1 region of Chikungunya virus in a cost‐effective manner

et al. 2020

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“…Some highlights from adaptations of LAMP include multiplexed LAMP, electrochemical LAMP, and disc-based LAMP [46][47][48]. For RNA viruses, such as ZIKV, DENV, and CHIKV, it is necessary to perform a reverse transcription reaction LAMP (RT-LAMP) [27,39,42,[49][50][51][52] which includes the enzymes that first convert RNA → DNA upstream of the LAMP process, unless a LAMP enzyme with both reverse transcriptase and DNA polymerase is used [53].…”

Section: Principles Of Lamp Assaymentioning

confidence: 99%

Loop-Mediated Isothermal Amplification (LAMP) for the Diagnosis of Zika Virus: A Review

Silva

Pardee

Pena

2019

Viruses

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The recent outbreak of Zika virus (ZIKV) in the Americas and its devastating developmental and neurological manifestations has prompted the development of field-based diagnostics that are rapid, reliable, handheld, specific, sensitive, and inexpensive. The gold standard molecular method for lab-based diagnosis of ZIKV, from either patient samples or insect vectors, is reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The method, however, is costly and requires lab-based equipment and expertise, which severely limits its use as a point-of-care (POC) tool in resource-poor settings. Moreover, given the lack of antivirals or approved vaccines for ZIKV infection, a POC diagnostic test is urgently needed for the early detection of new outbreaks and to adequately manage patients. Loop-mediated isothermal amplification (LAMP) is a compelling alternative to RT-qPCR for ZIKV and other arboviruses. This low-cost molecular system can be freeze-dried for distribution and exhibits high specificity, sensitivity, and efficiency. A growing body of evidence suggests that LAMP assays can provide greater accessibility to much-needed diagnostics for ZIKV infections, especially in developing countries where the ZIKV is now endemic. This review summarizes the different LAMP methods that have been developed for the virus and summarizes their features, advantages, and limitations.Viruses 2020, 12, 19 2 of 20 raising concerns about the neurological tropism of the virus [12]. In May 2015, the first case of ZIKV infection was reported in Brazil [13] and the virus rapidly spread throughout the country and much of Latin America, causing the largest recorded epidemic of the virus to date [14]. The Brazilian epidemic raised great international concern because of severe birth defects, including microcephaly, in neonates born to mothers infected by ZIKV during pregnancy [3,15,16].ZIKV is transmitted mainly through the bite of infected mosquitoes from the genus Aedes, although other vectors may also be involved in the transmission [17][18][19][20][21]. Additionally, other routes of ZIKV transmission have been identified, including blood transfusions, transplacental, perinatal, and sexual intercourse [22][23][24]. ZIKV infection usually causes a self-limiting and a mild illness, where the majority of cases are asymptomatic and, when present, symptoms include fever, headache, rash, conjunctivitis, and arthralgia [25]. In regions where there is a circulation of other arboviruses, such as DENV and chikungunya (CHIKV), the clinical diagnosis of ZIKV infection becomes extremely difficult because of common symptoms. Therefore, laboratory-based molecular diagnosis is of fundamental importance to correctly identify the etiologic agent [3,26].Given the lack of approved vaccines and antivirals against ZIKV, a rapid and reliable point-of-care (POC) diagnostic test for detection of ZIKV is urgently required for control and prevention measures and to increase the diagnostic capacity of ZIKV-affected, mainly in low-resource areas [27,28]. Z...

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“…Other molecular methods for detecting pathogenic viruses such as direct nucleic acid detection by a biosensor (Saylan et al, 2019) and Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) diagnostics using CRISPR-CRISPR-associated system (CRISPR-Cas) (Chertow, 2018) have also been developed. In addition, loop-mediated isothermal amplification (LAMP), a recent development of a diagnostic method offers advantages in terms of sensitivity, specificity, efficiency and rapidity and can be performed at the point of care for detection of emerging viruses (Notomi et al, 2000;Wong et al, 2017;Lopez-Jimena et al, 2018;Sabalza et al, 2018).…”

Section: Polymerase Chain Reactionmentioning

confidence: 99%

Pathogenic viruses: Molecular detection and characterization

Artika

Wiyatno

Ma’roef

2020

Infection, Genetics and Evolution

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Development of a single-tube one-step RT-LAMP assay to detect the Chikungunya virus genome

Cited by 45 publications

References 46 publications

Development of a portable reverse transcription loop‐mediated isothermal amplification system to detect the E1 region of Chikungunya virus in a cost‐effective manner

Development of a portable reverse transcription loop‐mediated isothermal amplification system to detect the E1 region of Chikungunya virus in a cost‐effective manner

Loop-Mediated Isothermal Amplification (LAMP) for the Diagnosis of Zika Virus: A Review

Pathogenic viruses: Molecular detection and characterization

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