Development of a spatially and timely resolved CFD model of a steam sterilizer to predict the load temperature and the theoretical inactivation of bacteria based on sterilization parameters

Feurhuber, Manuel; Burian, Paul; Magno, Marino; Miranda, Marco; Hochenauer, Christoph

doi:10.1016/j.phmed.2019.100020

Cited by 12 publications

(6 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…When comparing the temperature curves of the autoclave and microwave oven, a higher temperature was reached in both at 15-16 min [29]. However, the whole sterilization process was drastically reduced in the microwave method as, after 7 min, sterilization was achieved and the process of gas elimination was quicker, whereas the whole process in the autoclave, including the temperature increase, sterilization (commonly 21 min), and pressure decrease, may take up to 1.5 h [29]. Moreover, in the autoclave, higher pressures are likely to be reached than in the microwave system, due to the larger size of the autoclave and the autoclaving conditions.…”

Section: Discussionmentioning

confidence: 99%

Microwave Oven Application for the Preparation and Sterilization of Microbiological Culture Media: A Feasible Method with An Adapted Water Bath and Perforable Cap

Terrones-Fernandez,

Rodero-De-Lamo,

López

et al. 2024

Applied Sciences

View full text Add to dashboard Cite

There is currently increased interest in the use of alternatives to autoclaved culture media, in order to maintain the properties of the media, while saving energy and time. In this study, we assess a new system for culture media preparation, using a conventional microwave with a water bath and a glass bottle with a rubber cap that allows depressurization. Sterilization, using the proposed system (1000 W, 3 to 20 min), was compared with autoclaving for the preparation of tryptone soy agar (TSA), tryptone soy broth (TSB), Sabouraud 4% dextrose agar (SDA), and violet red bile glucose agar (VRBG). Microwave exposure for 7 min yielded sterile TSA plates. The productivity of both sterilization methods was assessed using the pour plate method, and significant increases in the growth of certain micro-organisms after using a microwave were observed for every culture medium, especially those that were sterilized by boiling (VRBG). The kinetics of microbial destruction showed that Escherichia coli and Bacillus subtilis spores were destroyed after 3 and 7 min in a microwave, respectively, while three decimal reductions were obtained for Geobacillus stearothermophilus spores after 15 min in an autoclave. This new sterilization method could be a feasible, rapid, and economical method to prepare microbiological media, with a quality similar to that obtained through autoclaving.

show abstract

Section: Discussionmentioning

confidence: 99%

Microwave Oven Application for the Preparation and Sterilization of Microbiological Culture Media: A Feasible Method with An Adapted Water Bath and Perforable Cap

Terrones-Fernandez,

Rodero-De-Lamo,

López

et al. 2024

Applied Sciences

View full text Add to dashboard Cite

show abstract

“…The determination of steam quality played a decisive role in this study. Comparable studies [12,17] that dealt with the flow simulation of steam sterilizers used a pressure boundary condition at the inlet. The calculated mass flow resulting from this pressure boundary condition can sometimes deviate greatly from the real mass flow.…”

Section: Measurement Of the Steam Mass Flow And Qualitymentioning

confidence: 99%

“…In order to be able to determine the heating process of the walls at all, these needed to be modeled as solids. Comparable applications [12,13,17] which also were used to perform CFD calculations for steam sterilizers neglected to model the chamber walls as solids due to the associated large temperature gradients and heat flows, which can inevitably lead to instabilities. For this reason, the model developed in the present work represents a major step towards providing an even more realistic simulation of steam sterilizers and, in general, of heating processes as a result of condensation in combination with solids.…”

Section: Computational Gridmentioning

confidence: 99%

Numerical Investigation of the Heating Process of the Walls in Steam Sterilizers

Pletzer

Miranda²,

Lucchesi³

et al. 2022

Chemie Ingenieur Technik

Self Cite

View full text Add to dashboard Cite

The basic principle of steam sterilization is based on high heat transfer rates from the steam to the medical device. In order to carry out this process as well as possible, the walls of the pressure chamber must be preheated. One way is to heat it with steam from the inside. To investigate this process, a three‐phase CFD model was developed, which can be used to determine the saturation temperature as a function of the partial pressure using a user‐defined function. For validation, the pressure, temperature (fluid and solid) and condensate content were measured. Results show that the model is excellently suited for predicting all relevant variables.

show abstract

Section: Thermal Sterilizationmentioning

confidence: 99%

“…The factors that define the operational conditions of such process include the type of steam (saturated or unsaturated), its temperature, the pressure in the autoclave, and the exposure time. [121] The minimum sterilization time depends on the type of object and its size to guarantee that all material in the autoclave has reached the required temperature. Therefore, temperature probes are used to monitor the sterilization process and cycle conditions.…”

Section: Moist Heatmentioning

confidence: 99%

Sterilization of Objects, Products, and Packaging Surfaces and Their Characterization in Different Fields of Industry: The Status in 2020

Jildeh

Wagner

Schöning

2021

Physica Status Solidi (a)

View full text Add to dashboard Cite

The treatment method to deactivate viable microorganisms from objects or products is termed sterilization. There are multiple forms of sterilization, each intended to be applied for a specific target, which depends on—but not limited to—the thermal, physical, and chemical stability of that target. Herein, an overview on the currently used sterilization processes in the global market is provided. Different sterilization techniques are grouped under a category that describes the method of treatment: radiation (gamma, electron beam, X‐ray, and ultraviolet), thermal (dry and moist heat), and chemical (ethylene oxide, ozone, chlorine dioxide, and hydrogen peroxide). For each sterilization process, the typical process parameters as defined by regulations and the mode of antimicrobial activity are summarized. Finally, the recommended microorganisms that are used as biological indicators to validate sterilization processes in accordance with the rules that are established by various regulatory agencies are summarized.

show abstract

Development of a spatially and timely resolved CFD model of a steam sterilizer to predict the load temperature and the theoretical inactivation of bacteria based on sterilization parameters

Cited by 12 publications

References 21 publications

Microwave Oven Application for the Preparation and Sterilization of Microbiological Culture Media: A Feasible Method with An Adapted Water Bath and Perforable Cap

Microwave Oven Application for the Preparation and Sterilization of Microbiological Culture Media: A Feasible Method with An Adapted Water Bath and Perforable Cap

Numerical Investigation of the Heating Process of the Walls in Steam Sterilizers

Sterilization of Objects, Products, and Packaging Surfaces and Their Characterization in Different Fields of Industry: The Status in 2020

Contact Info

Product

Resources

About