Development of a spiramycin sensor based on adsorptive stripping linear sweep voltammetry and its application for the determination of spiramycin in chicken egg samples

Abstract

Herein, an adsorptive stripping linear sweep voltammetric technique was described to determine spiramycin, a macrolide antibiotic, using a carboxylic multiwalled carbon nanotubes modified glassy carbon electrode. The main principle of the analytical methodology proposed was based on the pre-concentration of spiramycin by open-circuit accumulation of the macrolide onto the modified electrode surface. As a result of the adsorption affinity of spiramycin to the modified surface, the sensitivity of the glassy carbon electrode was significantly increased for the determination of spiramycin. The electrochemical behavior of spiramycin was evaluated by cyclic voltammetry and the irreversible anodic peak observed was measured as an analytical signal in the methodology. The proposed electrochemical sensing platform was quite linear in the range of 0.100-40.0 µM of spiramycin concentration with a correlation coefficient of 0.9993. The limit of detection and the limit of quantification were 0.028 and 0.094 µM, respectively. The intra and inter-day repeatability of the proposed sensor was within acceptable limits. Finally, the applicability of the electrochemical methodology was examined by determining the drug content of chicken egg samples spiked with spiramycin standard. A rapid and easy extraction technique was performed to extract spiked spiramycin from the egg samples. The extraction technique followed had good recovery values between 85.3 ± 4.0 and 93.4 ± 1.9%.

Green and cost-effective voltammetric assay for spiramycin based on activated glassy carbon electrode and its applications to urine and milk samples

“…The modified electrode exhibited a limit of detection (2.0 × 10 −8 M) that is ∼300-fold lower than the potentiometric method (5.9 × 10 −6 M), and ∼40, 500-fold lower than DPP and SWP, respectively. Compared with the recently developed Ad-SLSV assay, 23 the proposed sensor for SPA exhibited wider linear ranges (8.0 × 10 −8 to 8.5 × 10 −5 M and 8.5 × 10 −5 to 3.1 × 10 −4 M), a lower limit of detection (2.0 × 10 −8 M). The AD-SLSV assay of the studied drug, 23 however, exhibited ∼5-fold better sensitivity compared to the developed AGCE-based SPA electrode.…”

“…Compared with the recently developed Ad-SLSV assay, 23 the proposed sensor for SPA exhibited wider linear ranges (8.0 × 10 −8 to 8.5 × 10 −5 M and 8.5 × 10 −5 to 3.1 × 10 −4 M), a lower limit of detection (2.0 × 10 −8 M). The AD-SLSV assay of the studied drug, 23 however, exhibited ∼5-fold better sensitivity compared to the developed AGCE-based SPA electrode. The RP-HPLC-fluorescence method 12 showed a limit of the linear range (5.9 × 10 −8 M) lower than the modified electrode (8.0 × 10 −8 M).…”

“…S7,† the optimized AGCE current response remained stable for one week, after which, the current response decreased by 8.79%, which confirms the reasonable stability of the AGCE. It is worth mentioning that regeneration requires neither applying complicated procedures nor the use of expensive material, 23 but merely repeating the electrochemical activation procedures described above.…”

“…The fabrication procedures require expensive chemicals/reagents and consume ample time. 23 Moreover, this method has the disadvantage of using organic solvents ( e.g. , dimethylformamide) which were crucial for the fabrication procedures.…”

A Non‐Enzymatic and Electrochemical‐Based Sensor using a Prussian Blue‐Gold Nanoparticle‐Reduced Graphene Oxide Ternary Nanocomposite for Efficient Hydrogen Peroxide Detection.

“…The optimum stirring of the electrolyte may help increase the peak current response and for achieving a shorter time to reach the maximum current. [46] So, the current vs. time profile was plotted at different rpm rates in the stirred solution. The best results were obtained for the proposed sensor at near 220 rpm, which was selected throughout the study (Figure S8, c).…”