Development of a ssDNA aptamer for detection of residual benzylpenicillin

Lee, A‐Young; Ha, Na-Reum; Jung, In-Pil; Kim, Sang‐Heon; Kim, A-Ru; Yoon, Moon‐Young

doi:10.1016/j.ab.2017.05.013

Cited by 39 publications

(13 citation statements)

References 25 publications

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“…1). Fluorescein-labeled aptamers are quenched by rGO in the absence of their target (12). Addition of free target molecules posaconazole and itraconazole liberated the aptamer from the surface, which causes an increase in the fluorescent signal.…”

Section: Observationmentioning

confidence: 99%

A Novel, Rapid, and Low-Volume Assay for Therapeutic Drug Monitoring of Posaconazole and Other Long-Chain Azole-Class Antifungal Drugs

Wiedman

Zhao

Perlin

2018

mSphere

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This work describes an effective assay for TDM of long-chain azole-class antifungal drugs that can be used in diluted human serum samples. This assay will provide a quick, cost-effective method for monitoring concentrations of drugs such as posaconazole that exhibit well-documented pharmacokinetic variability. Our rGO-aptamer assay has the potential to improve health care for those struggling to treat fungal infections in rural or resource-limited setting.

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Section: Observationmentioning

confidence: 99%

A Novel, Rapid, and Low-Volume Assay for Therapeutic Drug Monitoring of Posaconazole and Other Long-Chain Azole-Class Antifungal Drugs

Wiedman

Zhao

Perlin

2018

mSphere

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“…S 5 , strip 3). Furthermore, we investigated acetic acid treatment of milk according to 27 ; even though CRP-biotin could be detected on the test line (Electronic Supplementary Material Fig. S 5 , strip 2), no signal decrease by ampicillin addition could be observed (results not shown).…”

Section: Resultsmentioning

confidence: 99%

Small molecule detection with aptamer based lateral flow assays: Applying aptamer-C-reactive protein cross-recognition for ampicillin detection

Kaiser

Weisser

Kohl

et al. 2018

Sci Rep

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Aptamer-based lateral flow assays (LFAs) are an emerging field of aptamer applications due to numerous potential applications. When compared to antibodies, potential advantages like cost effectiveness or lower batch to batch variations are evident. The development of LFAs for small molecules, however, is still challenging due to several reasons, primarily linked to target size and accessible interaction sites. In small molecule analysis, however, aptamers in many cases are preferable since immunogenicity is not required and they may exhibit even higher target selectivity. We report the first cross-recognition of a small molecule (ampicillin) and a protein (C-reactive protein), predicted by in-silico analysis, then experimentally confirmed - using two different aptamers. These features can be exploited for developing an aptamer-based LFA for label-free ampicillin detection, functioning also for analysis in milk extract. Most importantly, the principal setup denotes a novel, transferable and versatile general approach for detection of small molecules using competitive LFAs, unlikely to be generally realized by aptamer-DNA-binding otherwise.

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“…The enriched library of the eighth round was sequenced by HTS technology, which showed a total of 20,000 different sequences. Compared with the commonly used method (cloning to a vector) [25], HTS is an effective method to obtain rich sequence information in a short time. Then, Three hundred sequences with a higher frequency of occurrence were selected, and the random area of 40 base sequences was analyzed by clustal X2 ( Figure S1).…”

Section: High-throughput Sequencing and Sequence Analysis Of The Enrimentioning

confidence: 99%

Selection of Aptamers Specific for DEHP Based on ssDNA Library Immobilized SELEX and Development of Electrochemical Impedance Spectroscopy Aptasensor

et al. 2020

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A selection of aptamers specific for di(2-ethylhexyl) phthalate (DEHP) and development of electrochemical impedance spectroscopy (EIS) aptasensor are described in this paper. The aptamers were selected from an immobilized ssDNA library using the systematic evolution of ligands by exponential enrichment (SELEX). The enrichment was monitored using real-time quantitative PCR (Q-PCR), and the aptamers were identified by high-throughput sequencing (HTS), gold nanoparticles (AuNPs) colorimetric assay, and localized surface plasmon resonance (LSPR). The EIS aptasensor was developed to detect DEHP in water samples. After eight rounds of enrichment, HTS, AuNPs colorimetric assay, and LSPR analysis indicated that four aptamers had higher binding activity, and aptamer 31 had the highest affinity (Kd = 2.26 ± 0.06 nM). The EIS aptasensor had a limit of detection (LOD) of 0.103 pg/mL with no cross-reactivity to DEHP analogs and a mean recovery of 76.07% to 141.32% for detection of DEHP in water samples. This aptamer is novel with the highest affinity and sensitivity.

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Development of a ssDNA aptamer for detection of residual benzylpenicillin

Cited by 39 publications

References 25 publications

A Novel, Rapid, and Low-Volume Assay for Therapeutic Drug Monitoring of Posaconazole and Other Long-Chain Azole-Class Antifungal Drugs

A Novel, Rapid, and Low-Volume Assay for Therapeutic Drug Monitoring of Posaconazole and Other Long-Chain Azole-Class Antifungal Drugs

Small molecule detection with aptamer based lateral flow assays: Applying aptamer-C-reactive protein cross-recognition for ampicillin detection

Selection of Aptamers Specific for DEHP Based on ssDNA Library Immobilized SELEX and Development of Electrochemical Impedance Spectroscopy Aptasensor

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