Development of a syncytia inhibition assay for the detection of antibodies to bovine leukemia virus in naturally infected cattle; comparison with Western blot and agar gel immunodiffusion

Johnson, M J; Rommel, F. A.; Mone, Jay

doi:10.1016/s0166-0934(97)00186-9

Cited by 10 publications

(5 citation statements)

References 6 publications

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“…The BLV env gene encodes a precursor protein that is processed into two subunits, gp51, the outer membrane subunit, and gp30, the transmembrane subunit, both of which are essential for viral infectivity [20, 28]. A variety of diagnostic tests have been developed for BLV, including PCR-based assays, agar gel immunodiffusions (AGIDs), virus neutralization assays, and enzyme-linked immunosorbent assays (ELISAs) [1,9,11,12,16,25]. The most widely used diagnostic methods for the serological detection of BLV-specific antibodies are AGID and ELISA, which are antigen-based assays that use either wild type BLV gp51 isolated from fetal lamb kidney (FLK) cells, or a recombinant BLV antigen that is expressed in insect cells [6,16,19,23].…”

Section: Introductionmentioning

confidence: 99%

Agar gel immunodiffusion analysis using baculovirus-expressed recombinant bovine leukemia virus envelope glycoprotein (gp51/gp30T-)

et al. 2009

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Bovine leukemia virus (BLV) envelope glycoprotein (gp51/gp30T-), consisting of BLV gp51 and BLV gp30 that lacked its C-terminal transmembrane domain, was expressed in insect cells under the control of the baculovirus polyhedron promoter. Recombinant BLV gp51/gp30T- secreted from insect cells was determined by immunofluorescence, enzyme-linked immunosorbent and western blot assays using a BLV-specific monoclonal antibody and BLV-positive bovine antibodies. An agar gel immunodiffusion (AGID) test using gp51/gp30T- as the antigen for the detection of BLV antibodies in serum was developed and compared to traditional AGID, which uses wild type BLV antigen derived from fetal lamb kidney cells. AGID with the recombinant BLV gp51/gp30T- was relatively more sensitive than traditional AGID. When the two methods were tested with bovine sera from the field, the recombinant BLV gp51/gp30T- and traditional antigen had a relative sensitivity of 69.8% and 67.4%, respectively, and a relative specificity of 93.3% and 92.3%. These results indicated that the recombinant BLV gp51/gp30T- is an effective alternative antigen for the diagnosis of BLV infection in cattle.

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Section: Introductionmentioning

confidence: 99%

Agar gel immunodiffusion analysis using baculovirus-expressed recombinant bovine leukemia virus envelope glycoprotein (gp51/gp30T-)

et al. 2009

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“…Furthermore, ( Doménech et al., 1997 ) showed that only the serum from BLV-infected animals, which had the antibody to viral gp51, could inhibit the release of the virus from infected cells . The results reported by ( Doménech et al., 1997 ) suggest that the syncytia induction assay and the syncytia inhibition assay may be more sensitive than the Western blot and AGID assay systems for determining the infection status of cattle ( Johnson et al., 1998 ). According to identity values, BLV Iraqi strain may belong to genotype 1 due to the highly identity of Iraqi strain with Turkish strain that was genotype 1 ( Alkan, Oğuzoğlu, Timurkan, & Karapınar, 2011 ).…”

Section: Discussionmentioning

confidence: 99%

“…The syncytia inhibition assay was done in the same way as the syncytia induced assay, according to Johnson, Rommel, & Moné, (1998) . The FCK recipient cells were treated with 1 ml of anti-BLV sera and incubated for 48 h, and all the plates were incubated at 37 °C for three to four days.…”

Section: Methodsmentioning

confidence: 99%

Isolation of Bovine leukemia virus from cows with persistent lymphocytosis in Iraq

Khudhair

Al-Shammari

Hasso

et al. 2021

Veterinary and Animal Science

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Highlights This study includes the first report on the isolation of the BLV virus in Iraq ever. Cattle infected with PL can only be the source for viral isolation. Cytopathological features of the virus infection are differ depending on the cell type. The virus can be propagated in different cell types including human cells. This study describes first cellular and genotyping characterization for BLV virus Iraqi strain.

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“…Other diagnostic methods for BLV include the detection of BLV viral proteins through Western blotting (WB), syncytium formation assays in a co-culture, and indirect immunofluorescence (IF) for detecting BLV antigens [165][166][167][168][169][170]. PCR testing can be used to quantify the PVL of animals infected with BLV using molecular techniques.…”

Section: Molecular Techniques Used For the Diagnosis Of Blvmentioning

confidence: 99%

The Global Epidemiology of Bovine Leukemia Virus: Current Trends and Future Implications

Lv,

Wang,

Lian

et al. 2024

Animals

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Bovine leukemia virus (BLV) is a retrovirus that causes enzootic bovine leucosis (EBL), which is the most significant neoplastic disease in cattle. Although EBL has been successfully eradicated in most European countries, infections continue to rise in Argentina, Brazil, Canada, Japan, and the United States. BLV imposes a substantial economic burden on the cattle industry, particularly in dairy farming, as it leads to a decline in animal production performance and increases the risk of disease. Moreover, trade restrictions on diseased animals and products between countries and regions further exacerbate the problem. Recent studies have also identified fragments of BLV nucleic acid in human breast cancer tissues, raising concerns for public health. Due to the absence of an effective vaccine, controlling the disease is challenging. Therefore, it is crucial to accurately detect and diagnose BLV at an early stage to control its spread and minimize economic losses. This review provides a comprehensive examination of BLV, encompassing its genomic structure, epidemiology, modes of transmission, clinical symptoms, detection methods, hazards, and control strategies. The aim is to provide strategic information for future BLV research.

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Development of a syncytia inhibition assay for the detection of antibodies to bovine leukemia virus in naturally infected cattle; comparison with Western blot and agar gel immunodiffusion

Cited by 10 publications

References 6 publications

Agar gel immunodiffusion analysis using baculovirus-expressed recombinant bovine leukemia virus envelope glycoprotein (gp51/gp30T-)

Agar gel immunodiffusion analysis using baculovirus-expressed recombinant bovine leukemia virus envelope glycoprotein (gp51/gp30T-)

Isolation of Bovine leukemia virus from cows with persistent lymphocytosis in Iraq

The Global Epidemiology of Bovine Leukemia Virus: Current Trends and Future Implications

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