Development of a titanium dioxide nanoparticle pipette-tip for the selective enrichment of phosphorylated peptides

Binder-Free TiO2 Monolith-Packed Pipette Tips for the Enrichment of Phosphorylated Peptides

“…[10] Briefly, 1 mg of TiO 2 monolith was ground into small pieces and dispersed in 40 mL of methanol. Then, 0.2 mL of DPA and 25 mL of EDMA were added as the initiator and monomer.…”

“…[9] TiO 2 and other metal oxides have been packed in pipette tips as a convenient and frequently used device for in situ sample pretreatment. [8,[10][11][12] There are commercial pipette tips on the market (e.g. NuTip, Titansphere Phos-TiO Kit) employing embedded TiO 2 for p-peptide and protein enrichment.…”

“…The use of a functional tip in pretreatment is beneficial for minimizing sample loss, simplifying the operation procedures, and reducing the contamination risk. However, the packing of TiO 2 into the tip in literature reports is generally assisted by polymers for adhesion [10,12] or a plug [8] to prevent materials from leaking. The mix of adhesive polymer would reduce the functional TiO 2 content, which may result in a decrease in enrichment efficiency, and the use of a plug makes the packing procedure complicated.…”

An ICP-MS, ESI-MS and molecular modelling investigation of homogeneous gallium affinity tagging (HMAT) of phosphopeptides

“…[15][16][17][18][19][20][21][22][23][24][25][26][27][28][29] Many different analytical techniques have been developed to specifically enrich phosphopeptides including Immobilised Metal Affinity Chromatography (IMAC), utilizing iron, gallium, and more recently titanium and zirconium. [15,18,[29][30][31][32][33][34][35][36][37][38][39][40][41][42][43][44][45][46][47] The efficiency of each of these metals in selectively binding phosphopeptides has been extensively investigated many times with varying success. This has been attributed to the non-specific binding of the acidic amino acid residues asparagine and glutamine to the metal ions, the binding of hydrophobic residues to the gel bed and the elution of multiply phosphorylated peptides and impurities in the metal oxide material.…”

Ultra-Specific Enrichment of GST-Tagged Protein by GSH-Modified Nanoparticles

“…Improving techniques that allow enrichment with high specificity and efficiency towards lesser abundant subsets of the proteome would be a significant advancement, with useful applications in, for example, finding patient-specific disease biomarkers from plasma. [8][9][10][11][12] Enrichment techniques based on interactions of small molecules with enzymes might provide valuable information for drug discovery and personalized cancer therapy. [13][14][15][16][17][18][19][20][21] Conventionally, target proteins have been captured by specific interactions on micron sized beads surface-modified by small molecules.…”