Development of a Triple-Coaxial Flow Device for Fabricating a Hydrogel Microtube and Its Application to Bioremediation

Fujimoto, Keiichiro; Higashi, Kazuhiko; Onoe, Hiroaki; Miki, Norihisa

doi:10.3390/mi9020076

Cited by 9 publications

(6 citation statements)

References 23 publications

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“…More complicated microfluidic devices can be used to obtain structures such as core-shell spheres, hollow tubes, osteon-like microfibers (Fig. 3a), and Janus particles (particles with two defined and unique surfaces) with applications in drug delivery and scaffolds for multilayered cell systems [119][120][121][122].…”

Section: Microfluidicsmentioning

confidence: 99%

“…One of the biggest limitations of microfluidics is the volume that can be processed over time. In the systems already described, the flow rates range from an average of 30 mL h −1 for fibers, 10 mL h −1 for solid spheres, and up to 300 μL h −1 for multicomponent spherical shapes [119][120][121][122][123]. Based on this flow rates, the more complicated the shape and the more components it has (Fig.…”

Section: Microfluidicsmentioning

confidence: 99%

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Methods for producing microstructured hydrogels for targeted applications in biology

Garcia

Kiick

2019

Acta Biomaterialia

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Hydrogels have been broadly studied for applications in clinically motivated fields such as tissue regeneration, drug delivery, and wound healing, as well as in a wide variety of consumer and industry uses. While the control of mechanical properties and network structures are important in all of these applications, for regenerative medicine applications in particular, matching the chemical, topographical and mechanical properties for the target use/tissue is critical. There have been multiple alternatives developed for fabricating materials with microstructures with goals of controlling the spatial location, phenotypic evolution, and signaling of cells. The commonly employed polymers such as poly(ethylene glycol) (PEG), polypeptides, and polysaccharides (as well as others) can be processed by various methods in order to control material heterogeneity and microscale structures. We review here the more commonly used polymers, chemistries, and methods for generating microstructures in biomaterials, highlighting the range of possible morphologies that can be produced, and the limitations of each method. With a focus in liquidliquid phase separation, methods and chemistries well suited for stabilizing the interface and arresting the phase separation are covered. As the microstructures can affect cell behavior, examples of such effects are reviewed as well.

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Section: Microfluidicsmentioning

confidence: 99%

Section: Microfluidicsmentioning

confidence: 99%

Methods for producing microstructured hydrogels for targeted applications in biology

Garcia

Kiick

2019

Acta Biomaterialia

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“…Using our needle-in-needle, device-based method, we can not only encapsulate cells in hydrogel microtubes, but also fabricate pre-formed hydrogel microtubes as an “off-the-shelf” 3D culture system that allows injection of cell types of interest at any cell seeding density and cell ratio for co-culture or multi-culture. In particular, by using alginate hydrogel microtubes, the hollow core offers a non-restricted space, allowing cell migration and aggregation and providing access to nutrients in the immediate vicinity of cell clusters [ 42 , 43 , 44 ]. The use of 6% sodium alginate makes these hydrogel microtubes strong enough to be handled for cell injection and long-term cell culture compared to conventional hydrogel microtubes made of low viscosity sodium alginate [ 45 ] or low concentration (e.g., 1–2%) sodium alginate [ 31 , 32 , 33 ].…”

Section: Discussionmentioning

confidence: 99%

Alginate Hydrogel Microtubes for Salivary Gland Cell Organization and Cavitation

et al. 2022

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Understanding the different regulatory functions of epithelial and mesenchymal cell types in salivary gland development and cellular organization is essential for proper organoid formation and salivary gland tissue regeneration. Here, we demonstrate a biocompatible platform using pre-formed alginate hydrogel microtubes to facilitate direct epithelial–mesenchymal cell interaction for 3D salivary gland cell organization, which allows for monitoring cellular organization while providing a protective barrier from cell-cluster loss during medium changes. Using mouse salivary gland ductal epithelial SIMS cells as the epithelial model cell type and NIH 3T3 fibroblasts or primary E16 salivary mesenchyme cells as the stromal model cell types, self-organization from epithelial–mesenchymal interaction was examined. We observed that epithelial and mesenchymal cells undergo aggregation on day 1, cavitation by day 4, and generation of an EpCAM-expressing epithelial cell layer as early as day 7 of the co-culture in hydrogel microtubes, demonstrating the utility of hydrogel microtubes to facilitate heterotypic cell–cell interactions to form cavitated organoids. Thus, pre-formed alginate microtubes are a promising co-culture method for further understanding epithelial and mesenchymal interaction during tissue morphogenesis and for future practical applications in regenerative medicine.

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“…Herein, we prepared hydrogels in the shape of double-walled microtubes using triple channel microfluidic devices via photopolymerization with a calcium-alginate (Ca-Alg) template. It should be noted that the production of hydrogel microtubes using triple channel microfluidic devices has been previously reported, ,− but these reports dealt with single wall microtubes or double-walled microtube hydrogels without an inner hollow section or could not be produced continuously. In contrast to previous reports, triple channels allowed the injection of two different pregel solutions incorporated with sodium alginate (Na-Alg).…”

Section: Introductionmentioning

confidence: 99%

Temperature-Controllable Hydrogels in Double-Walled Microtube Structure Prepared by Using a Triple Channel Microfluidic System

Imani

Kim

et al. 2018

Langmuir

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Hydrogels in the shape of double-walled microtubes possess great potential for development into artificial human blood vessels. In this work, we have prepared temperature-responsive tubular hydrogels with selectively controllable wall diameters, by using alginate templated photopolymerization in a triple channel microfluidic device. These tubular hydrogels mimic human blood vessels because of the separate thermally active inner and passive outer walls. The different behavior of each wall leads to the expansion of the hollow center volume with increasing temperature. This temperature-based control of the hollow center volume cannot be achieved in the case of conventional hydrogel microtubes. Furthermore, through this method, the hydrogels can be modified to achieve a controllable outer diameter while maintaining the hollow center dimensions simply by changing the position of the hydrogel walls. The ability to change the layer properties of the developed system indicates that the preparation of hydrogels with various monomers is possible.

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Development of a Triple-Coaxial Flow Device for Fabricating a Hydrogel Microtube and Its Application to Bioremediation

Cited by 9 publications

References 23 publications

Methods for producing microstructured hydrogels for targeted applications in biology

Methods for producing microstructured hydrogels for targeted applications in biology

Alginate Hydrogel Microtubes for Salivary Gland Cell Organization and Cavitation

Temperature-Controllable Hydrogels in Double-Walled Microtube Structure Prepared by Using a Triple Channel Microfluidic System

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