Development of a Valencia Orange Pectin Methylesterase for Generating Novel Pectin Products

Savary, Brett J.; Hotchkiss, Arland T.; Fishman, Marshall L.; Cameron, Randall G.; Shatters, Robert G.

doi:10.1007/978-94-017-0331-4_26

Cited by 24 publications

(32 citation statements)

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“…The pH and salts were able to depolymerize and break of macromolecular substance. 31,32 When processing the articially stimulated GI digestion, found that the pH was the major cause for the signicant decrease in molecular weight of Plantago asiatica L., and the variations of molecular weight were in line with our results.…”

supporting

confidence: 79%

Artificial simulation of salivary and gastrointestinal digestion, and fermentation by human fecal microbiota, of polysaccharides fromDendrobium aphyllum

et al. 2018

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In vitro salivary and gastrointestinal (GI) digestion and fermentation of polysaccharides extracted from Dendrobium aphyllum were investigated in this study. Salivary amylase showed no influence on D.aphyllum polysaccharides (DAP). The molecular weight of DAP decreased dramatically during the first 0.5 h of gastric digestion, and then reduced steadily during the subsequent GI tract consumption. The content of reducing sugars increased steadily during GI digestion. Only released free mannose of DAP was detectable by gas chromatography-mass spectrometry analysis during the first 12 h of fermentation, which was contributed by fecal microbiota metabolism. In terms of the fermentation pattern, the pH dropped significantly due to the formation of six types of short-chain fatty acids (SCFAs). This study demonstrates that polysaccharides extracted from D. aphyllum can be digested by the GI tract and are physiologically active in the human large bowel by lowering the pH of the large intestinal environment and promoting the production of SCFAs.

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supporting

confidence: 79%

Artificial simulation of salivary and gastrointestinal digestion, and fermentation by human fecal microbiota, of polysaccharides fromDendrobium aphyllum

et al. 2018

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“…97,184 However, its functionality is highly dependent on the degree and pattern of methoxylation, which can be engineered by PMEs with specific modes of action under controlled reaction conditions. 15,34,90 Finally, pectinases, among which PME, are used for numerous other applications, also outside the food industry for treatment of pectin-rich material, for example, in textile processing, retting and degumming of fibre crops, pectic waste water treatment (e.g., from the citrus-processing industry), paper making, animal feed production, coffee and tea fermentation, oil extraction and biomass saccharification. 140,182,185…”

Section: Exogenous Pmementioning

confidence: 99%

“…Section 2.3.3) have been demonstrated. 21,[33][34][35] Relative proportions of PME isoforms may vary greatly according to the developmental stage and the organ considered. 16 Some isoforms are constitutively expressed throughout the plant, 29,36,37 whereas others are specific for one tissue (e.g., flowers, fruits or roots) and/or precise developmental stage (e.g., fruit ripening, microsporogenesis, germination or stem elongation).…”

Section: Polymorphismmentioning

confidence: 99%

Pectin methylesterase and its proteinaceous inhibitor: a review

Jolie

Duvetter

Loey

et al. 2010

Carbohydrate Research

278

178

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“…Unfortunately, this process is accompanied by depolymerisation of pectin, which could induce a change in gel properties. As an alternative, demethoxylation could be performed by use of pectinmethylesterase (PME), which offers the advantage that depolymerisation is avoided (Savary, Hotchkiss, Fishman, Cameron, & Shatters, 2003). Furthermore, enzymatic demethoxylation gives rise to a different pattern of methoxylation, i.e.…”

Section: Introductionmentioning

confidence: 99%