Development of a zebrafish embryo teratogenicity assay and quantitative prediction model

Brannen, Kimberly C.; Panzica-Kelly, Julieta M.; Danberry, Tracy L.; Augustine-Rauch, Karen

doi:10.1002/bdrb.20223

Cited by 278 publications

(190 citation statements)

References 48 publications

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“…[19] Moreover,e cologically relevant endpoints such as hatching, malformation, organ dysfunction and survival can providec lear evidenceo ft he teratogenic toxicity of the tested compounds. [20] Firstly,t he hatchability of zebrafish embryos treated with the pesticides (NTX and THI, respectively)i nt he absence andp resence of CB [7] was evaluated.A ss hown in Figure 2, both NTX and THI, at 3 mm and 10 mm after 48 hi ncubation with zebrafish embryos, induceds ignificantly retarded embryonic hatching, and the zebrafish embryo exposed to higher concentrations (! 30 mm)o ft hese pesticides for 48 hh ad noneh atched by the end of incubation, suggesting that both NTX and THI exhibitedd ramatic developmental toxicity on zebrafish embryos.…”

Section: Resultsmentioning

confidence: 99%

Constraining the Teratogenicity of Pesticide Pollution by a Synthetic Nanoreceptor

Yang

Wang

et al. 2017

Chemistry An Asian Journal

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The teratogenicity of the pesticide nereistoxin (NTX) and its derivativet hiocyclam (THI) towards aquatic life was dramatically constrained by as ynthetic nanoreceptor, cucurbit [7]uril, through selectivee ncapsulation of the pesticides (K CB [7]-NTX of 3.24(AE 0.31) 10 6 m À1 and K CB [7]-THI of 7.46(AE 0.10) 10 5 m À1 ), as evidenced by the rate of hatchability,morphologyd evelopment, and tyrosinase activity of zebrafish larvae incubated with the pesticides( 3-300 mm)i nt he absence and in the presence of 300 mm cucurbit [7]uril, demonstratingthe significant potential of the nanoreceptorinmanaging ecological pollution of these pesticides.

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Section: Resultsmentioning

confidence: 99%

Constraining the Teratogenicity of Pesticide Pollution by a Synthetic Nanoreceptor

Yang

Wang

et al. 2017

Chemistry An Asian Journal

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“…Numerous authors have conducted tests concerning the early development stages of zebra fish, all revealing an increased sensitivity of this test to estimate: the maximum acceptable concentration of a toxin [2][3][4][6][7][8], developmental neurotoxicity [24], teratogenicity [5,23], embryotoxicity [3,25], and/or screening a large number of chemicals [10,13,14,26].…”

Section: Discussionmentioning

confidence: 99%

Use of Zebra Fish Eggs as Early Indicators of Aquatic Environmental Pollution

Moşneang¹,

Dumitrescu²,

Муселин³

et al. 2015

Pol. J. Environ. Stud.

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“…Among other small-bodied species, such as the fathead minnow Pimephales promelas (Panter et al 2000, Johns et al 2011 and Japanese medaka Oryzias latipes (Melo andRamsdell 2001, Han et al 2010), it is routinely used for basic and applied endocrine studies of chemicals used in the environment and environmental samples. With the flexibility of assessment endpoints, life-stages of zebrafish from post-fertilized egg through embryonic, juvenile up to adult animals have been widely used to study developmental (Kimmel et al 1995), mutational (Incardona et al 2004), teratogenic (Brannen et al 2010) and reproductive (Andersen et al 2003, Van den Belt et al 2003, Segner et al 2003 impacts among other toxicity investigations. Specifically, endocrine status has also been widely studied in zebrafish ELS , Andersen et al 2003, Brion et al 2004, Kazeto et al 2004, Muncke and Eggen 2006.…”

Section: General Informationmentioning

confidence: 99%

Probing the xeno-oestrogenic impact of sediment cores contaminated by the pulp and paper industry: induction of aromatase cyp19a1b in late larval zebrafish

Sahoo

Oikari

2014

J Soils Sediments

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To assess xenoestrogenicity, the 20 days-post-fertilization zebrafish (20dpfZF) life-stage was experimentally exposed to known model chemicals as well as to environmentally contaminated samples. From a selection of key responsive genes, molecular responses associated with the steroidogenic impact (brain aromatase, cyp19a1b and vitellogenin isoform 1, vtg1) were used to assess the endocrinic modulation by xenoestrogenic samples. This work showed diverse in vivo responses by the 20dpfZF, from pure chemicals (bisphenol A, BPA; 17 -ethinylestradiol, EE2; nonylphenol, NP; octylphenol, OP), and their mixture combinations, and to industrially contaminated sediment samples. Gene expression analysis of cyp19a1b and vtg1 in exposed 20dpfZF showed the estrogenic potencies of chemicals in the order: EE2 > OP > BPA > NP. Transcriptional up-regulation of vtg1 in early juvenile fish exposed to mixtures (BPA + NP + OP), relative to individual concentrations, suggest in minimum the additive in vivo effect of a mixture of very low sublethal doses of single xenoestrogens. Complementary expression of cyp19a1b and vtg1 in 20dpfZF, exposed to artificially-spiked sediment with EE2 established the estrogenresponsiveness of the model. In contrast, the lack of simple estrogenic or antiestrogenic trait of correspondence in biomarker transcription was evident from exposure to two types of industrially contaminated sediments, suggesting common non-linearity of in vivo response by the model. The results also highlight the applicability of 20dpfZF in the assessment of remediation and monitoring of historically polluted sites. This study showed that the 20dpfZF addressed the regulatory requirement of a multi-purpose and cost-effective ecotoxicological model capable of in vivo genomic assessment of endocrineactive substances in aquatic samples.

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Development of a zebrafish embryo teratogenicity assay and quantitative prediction model

Abstract: The results indicate that this assay is promising for screening compounds for teratogenic potential. Birth Defects Res (Part B) 89:66-77, 2010. (c) 2010 Wiley-Liss, Inc.

Cited by 278 publications

References 48 publications

Constraining the Teratogenicity of Pesticide Pollution by a Synthetic Nanoreceptor

Constraining the Teratogenicity of Pesticide Pollution by a Synthetic Nanoreceptor

Use of Zebra Fish Eggs as Early Indicators of Aquatic Environmental Pollution

Probing the xeno-oestrogenic impact of sediment cores contaminated by the pulp and paper industry: induction of aromatase cyp19a1b in late larval zebrafish

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