Development of AFLP markers and phylogenetic analysis in Hypsizygus marmoreus

Abstract

The present study was designed to develop a reliable experimental protocol for detecting and genotyping amplifi ed fragment length polymorphism (AFLP) markers in the commercially important edible mushroom Hypsizygus marmoreus; and to use the markers to evaluate the genetic polymorphisms and phylogenetic relationships among 19 strains of H. marmoreus. Using a set of ten primer pairs, 609 AFLP markers were identifi ed, of which 532 were found segregating among 19 cultivated varieties and laboratory stock strains. An estimate of genetic relationships among the varieties/strains based on the AFLP markers was obtained by primary phylogenetic analysis using UPGMA. Our data demonstrate abundant genetic variation in the natural population of H. marmoreus and facilitate further analysis of genetic divergence within the species. The present study is useful for understanding the genetic structure of this species and uncovering genetic control of economically important traits for effi cient breeding programs.

Construction of a Genetic Linkage Map Based on Amplified Fragment Length Polymorphism Markers and Development of Sequence-Tagged Site Markers for Marker-Assisted Selection of the Sporeless Trait in the Oyster Mushroom (Pleurotus eryngii)

“…MAS using strain-specific markers has frequently been studied in edible mushrooms (44,54,55,58), but there have been few reports of MAS for agronomic traits in mushroom breeding (38,47). Here we established MAS based on a genetic linkage map for the dominant sporeless trait in P. eryngii.…”

“…Its practical application for mushroom breeding lags behind that for plants and animals. With mushrooms, although molecular markers have been one of the most important tools used to differentiate cultivars for the protection of breeder's rights (44,54,55,58), reports on the utilization of agronomic traits for breeding are few (38,47). The reasons include the lack of necessary materials, such as a genetic linkage map for the establishment of molecular breeding.…”

The complete mitochondrial genome of the Basidiomycete edible fungus Hypsizygus marmoreus

“…Some studies indicated that strains of H. marmoreus show abundant diversities in morphological and genetic characters. PCR-based molecular markers and inter-simple sequence repeat (ISSR) have been widely used in genetic studies of H. marmoreus (Wang et al 2009;Qiu et al 2013). However, there were no enough polymorphic sites for identifying different strains.…”

Genetic Diversity Analysis ofHypsizygus marmoreuswith Target Region Amplification Polymorphism

“…Molecular markers can directly reflect the genetic polymorphisms in DNA levels and have been widely used to analyze the genetic diversities, construct the genetic linkage maps, identify strains, and assist in breeding [8, 9]. In the previous studies, the amplified fragment length polymorphism (AFLP) markers and sequence characterized amplified region (SCAR) markers were developed to discriminate the strains and analyse the genetic divergences among the strains of H. marmoreus [10, 11]. Although a few studies on molecular markers of H. marmoreus have been carried out, the tested strains and the applied analysis methods were limited [12–14].…”