Development of an acetylcholinesterase–choline oxidase based biosensor for acetylcholine determination

Tunç, Ayşe Tuğçe; Koyuncu, Elif Aynaci; Arslan, Fatma

doi:10.3109/21691401.2015.1080167

Cited by 23 publications

(6 citation statements)

References 41 publications

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“…The electrochemical response observed at the proposed biosensor surface was based on the hydrolysis of ACh into thiocholine (TCh) and acetic acid by AChE (Figure S12). 48,49 The current generated by the oxidation of TCh is proportional to the substrate ACh concentration. The ZnS/ ZnO/Ta 2 O 5 −SiO 2 CSNPs exhibited better interface capabilities and conductivity owing to synergistic effects arising from the inclusion of ZnS, ZnO, and Ta 2 O 5 .…”

Section: Resultsmentioning

confidence: 99%

Gradient Triple-Layered ZnS/ZnO/Ta₂O₅–SiO₂ Core–Shell Nanoparticles for Enzyme-Based Electrochemical Detection of Cancer Biomarkers

Murugan

Sundramoorthy

et al. 2020

ACS Appl. Nano Mater.

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We report a methodology to fabricate unique threelayered ZnS/ZnO/Ta 2 O 5 (core)−SiO 2 (shell) nanoparticles (CSNPs) with varying compositions of ZnS, ZnO, Ta 2 O 5 , and SiO 2 in their layers (i.e., gradient structures). An electrochemical approach with better specificity and detection limit for nonneurally secreted cancer biomarker, acetylcholine (ACh), by modifying the glassy carbon electrode surface with enzyme tagged ZnS/ZnO/Ta 2 O 5 −SiO 2 CSNPs is reported. The size, composition, porosity, and morphology of CSNPs were characterized by using scanning electron microscopy, transmission electron microscopy, Raman spectroscopy, Brunauer−Emmett−Teller (BET) surface area analysis, X-ray diffraction technique, and Fourier-transform infrared spectroscopy. A detailed discussion on the mechanism of core−shell formation and the role of ZnCl 2 in the core−shell formation is reported. We also demonstrate that the detection limit of ACh could be significantly improved by controlling the thickness of outermost shell layer. Electrochemical investigations performed using differential pulse voltammetry and cyclic voltammetry techniques displayed that the oxidation current of ACh was higher for CSNPs synthesized at 0.15 wt % of ZnCl 2 concentration. The limit of detection of ACh was found to be 11.6 nM for CSNPs prepared at 0.15 wt % ZnCl 2 concentration. Thus, the CSNPs-based electrochemical sensor platform has high potential for developing a simple, cost-effective biosensor for early detection of cancer biomarker at nanomolar concentration.

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Section: Resultsmentioning

confidence: 99%

Gradient Triple-Layered ZnS/ZnO/Ta₂O₅–SiO₂ Core–Shell Nanoparticles for Enzyme-Based Electrochemical Detection of Cancer Biomarkers

Murugan

Sundramoorthy

et al. 2020

ACS Appl. Nano Mater.

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“…found in body fluid is quite high at high potentials [12]. Therefore, +0.4 V, which provides high response current and linearity, was determined as the most suitable working potential [5, 13].…”

Section: Resultsmentioning

confidence: 99%

A sensitive amperometric detection of neurotransmitter acetylcholine using carbon dot‐modified carbon paste electrode

Bodur

Dinç

Özmen

et al. 2020

Biotech and App Biochem

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Acetylcholine is a neurotransmitter, which is located at the intersections of the nerve and muscles in the lymph nodes of the internal organs motor systems and in various parts of the central nervous system. A decrease of acetylcholine in brain is associated with Alzheimer‘s disease. That is why it is an important agent for this disease. In this study, a bienzymatic biosensor system with acetylcholine esterase and choline oxidase was prepared with carbon paste electrode modified with carbon nano Dot‐(3‐Aminopropyl) triethoxysilane (CDs‐APTES) for determination of the amount of acetylcholine. Acetylcholine esterase and choline oxidase enzymes were immobilized onto a modified carbon paste electrode by cross‐linking with glutaraldehyde. Determination of acetylcholine was carried out by the oxidation of enzymatically produced H2O2 at 0.4 V versus Ag/AgCl. The effect of temperature, pH, and substrate concentration on the acetylcholine response of the prepared biosensor was investigated. In addition, the optimum CDs‐APTES amount, the linear operating range of the biosensor, and the interference effect were also investigated.

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“…Choline (Ch) and its coenzyme A ester (acetyl CoA) are converted to ACh (CH 3 COOCH 2 CH 2 N + (CH 3 ) 3 by an enzyme known as acetylcholine transferase. ACh, scientifically referred to as 2-acetoxy-N, N, N-trimethylethanaminium, is an important neurotransmitter in which both the central and peripheral nervous systems (PNS) are involved [ 1 ]. Henry Hallett Dale first discovered it in 1914, and Otto Loewi subsequently verified its existence in 1922.…”

Section: Introductionmentioning

confidence: 99%

An Amperometric Acetylcholine Biosensor Based on Co-Immobilization of Enzyme Nanoparticles onto Nanocomposite

2023

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An electrochemical biosensor was fabricated using nanoparticles of acetylcholinesterase (AChE) and choline oxidase (ChO)/Pt nanoparticles (PtNPs)/porous graphene oxide nanosheet (GONS) composite. A pencil graphite electrode (PGE) was used for the electrodeposition of nanocomposite and the determination of acetylcholine (ACh), a neurotransmitter. Various techniques such as scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), Fourier-transform infrared (FTIR) spectra and cyclic voltammetry (CV) were used for characterization. This biosensor (AChENPs-ChONPs/GONS/PtNPs/PGE) indicated a very short response time (3 s), a lower limit of detection (0.001 µM), good linearity (0.001–200 µM), longer storage stability (6 months) and better reproducibility. The percent analytical recoveries of added acetylcholine in serum (5.0 and 10 µM) were found to be 97.6 ± 0.7 and 96.5 ± 0.3 for the present biosensor. The coefficients of variation were obtained to be 8% and 3.25%, correspondingly. The biosensor was applied to measure the ACh amount in the serum of healthy individuals and patients with Alzheimer’s disease. The number of interferents had no effect on the biosensor at their physiological concentrations.

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Development of an acetylcholinesterase–choline oxidase based biosensor for acetylcholine determination

Cited by 23 publications

References 41 publications

Gradient Triple-Layered ZnS/ZnO/Ta₂O₅–SiO₂ Core–Shell Nanoparticles for Enzyme-Based Electrochemical Detection of Cancer Biomarkers

Gradient Triple-Layered ZnS/ZnO/Ta₂O₅–SiO₂ Core–Shell Nanoparticles for Enzyme-Based Electrochemical Detection of Cancer Biomarkers

A sensitive amperometric detection of neurotransmitter acetylcholine using carbon dot‐modified carbon paste electrode

An Amperometric Acetylcholine Biosensor Based on Co-Immobilization of Enzyme Nanoparticles onto Nanocomposite

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Development of an acetylcholinesterase–choline oxidase based biosensor for acetylcholine determination

Cited by 23 publications

References 41 publications

Gradient Triple-Layered ZnS/ZnO/Ta2O5–SiO2 Core–Shell Nanoparticles for Enzyme-Based Electrochemical Detection of Cancer Biomarkers

Gradient Triple-Layered ZnS/ZnO/Ta2O5–SiO2 Core–Shell Nanoparticles for Enzyme-Based Electrochemical Detection of Cancer Biomarkers

A sensitive amperometric detection of neurotransmitter acetylcholine using carbon dot‐modified carbon paste electrode

An Amperometric Acetylcholine Biosensor Based on Co-Immobilization of Enzyme Nanoparticles onto Nanocomposite

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Gradient Triple-Layered ZnS/ZnO/Ta₂O₅–SiO₂ Core–Shell Nanoparticles for Enzyme-Based Electrochemical Detection of Cancer Biomarkers

Gradient Triple-Layered ZnS/ZnO/Ta₂O₅–SiO₂ Core–Shell Nanoparticles for Enzyme-Based Electrochemical Detection of Cancer Biomarkers