Development of an AFLP based linkage map of Ler, Col and Cvi Arabidopsis thaliana ecotypes and construction of a Ler/Cvi recombinant inbred line population

Alonso‐Blanco, Carlos; Peeters, Anton J. M.; Koornneef, M.; Lister, Clare; Dean, Caroline; Bosch, Nina; Pot, J.; Kuiper, Martin

doi:10.1046/j.1365-313x.1998.00115.x

Cited by 346 publications

(358 citation statements)

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“…This placed GS-AOP on three possible bacterial artificial chromosomes (BACs): F4C21, T4I9, and T5J8. Analysis of the L er ϫ Cvi RILs amplified fragment length polymorphism (AFLP) map also placed GS-ALK and GS-OHP at the top of chromosome IV (5-centimorgan centromeric from GS.250C and 17-centimorgan telomeric of CH.169.C; Alonso-Blanco et al, 1998b). Fine-scale mapping in this cross placed GS-AOP on the same BACs as those identified in the L er ϫ Col cross (Figure 3).…”

Section: Fine-scale Mapping Of Gs-aopmentioning

confidence: 77%

“…Both the GS-ALK and GS-OHP variants map to the top of chromosome IV; thus, we refer to them jointly as GS-AOP . To map the GS-AOP region, we used 300 lines of the Landsberg erecta (L er ) ϫ Columbia (Col) recombinant inbred lines (RILs) and 162 lines of the L er ϫ Cape Verde Islands (Cvi) RILs (Lister and Dean, 1993;Alonso-Blanco et al, 1998b). L er contains the GS-OHP product 3-hydroxypropyl glucosinolate as the predominant short-chain aliphatic glucosinolate, and Cvi contains the GS-ALK products allyl glucosinolate and 3-butenyl glucosinolate (Figure 2).…”

Section: Cosegregation Of Gs-alk Gs-ohp and Gs-aop Nullmentioning

confidence: 99%

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Gene Duplication in the Diversification of Secondary Metabolism: Tandem 2-Oxoglutarate–Dependent Dioxygenases Control Glucosinolate Biosynthesis in Arabidopsis

et al. 2001

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Secondary metabolites are a diverse set of plant compounds believed to have numerous functions in plant-environment interactions. The large chemical diversity of secondary metabolites undoubtedly arises from an equally diverse set of enzymes responsible for their biosynthesis. However, little is known about the evolution of enzymes involved in secondary metabolism. We are studying the biosynthesis of glucosinolates, a large group of secondary metabolites, in Arabidopsis to investigate the evolution of enzymes involved in secondary metabolism. Arabidopsis contains natural variations in the presence of methylsulfinylalkyl, alkenyl, and hydroxyalkyl glucosinolates. In this article, we report the identification of genes encoding two 2-oxoglutarate-dependent dioxygenases that are responsible for this variation. These genes, AOP2 and AOP3 , which map to the same position on chromosome IV, result from an apparent gene duplication and control the conversion of methylsulfinylalkyl glucosinolate to either the alkenyl or the hydroxyalkyl form. By heterologous expression in Escherichia and the correlation of gene expression patterns to the glucosinolate phenotype, we show that AOP2 catalyzes the conversion of methylsulfinylalkyl glucosinolates to alkenyl glucosinolates. Conversely, AOP3 directs the formation of hydroxyalkyl glucosinolates from methylsulfinylalkyl glucosinolates. No ecotype coexpressed both genes. Furthermore, the absence of functional AOP2 and AOP3 leads to the accumulation of the precursor methylsulfinylalkyl glucosinolates. A third member of this gene family, AOP1 , is present in at least two forms and found in all ecotypes examined. However, its catalytic role is still uncertain.

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Section: Fine-scale Mapping Of Gs-aopmentioning

confidence: 77%

Section: Cosegregation Of Gs-alk Gs-ohp and Gs-aop Nullmentioning

confidence: 99%

Gene Duplication in the Diversification of Secondary Metabolism: Tandem 2-Oxoglutarate–Dependent Dioxygenases Control Glucosinolate Biosynthesis in Arabidopsis

et al. 2001

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“…The RIL population derived from crosses between the laboratory strain Ler and the accession Cvi was used. These RILs have previously been genotyped with AFLP and CAPS markers (32).…”

Section: Methodsmentioning

confidence: 99%

Vacuolar invertase regulates elongation of Arabidopsis thaliana roots as revealed by QTL and mutant analysis

Сергеева

Keurentjes

Bentsink

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

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179

128

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The possible role of the sucrose-splitting enzymes sucrose synthase and invertase in elongating roots and hypocotyls of Arabidopsis was tested by using a combination of histochemical methods and quantitative trait locus (QTL) analysis. Lengths of roots and hypocotyls correlated better with invertase activities than with sucrose synthase activities. The highest correlations were observed with activities in the elongating zones of roots. The genetic basis of these correlations was studied by using QTL analysis. Several loci, affecting invertase activity, colocated with loci that had an effect on root or hypocotyl length. Further fine mapping of a major locus for root length, but not for hypocotyl length (top chromosome 1), consistently showed colocation with the locus for invertase activity containing a gene coding for a vacuolar invertase. The analysis of a functional knockout line confirmed the role of this invertase in root elongation, whereas other invertase genes might play a role in hypocotyl elongation. Thus, we show the power of QTL analysis, combined for morphological and biochemical traits, followed by fine-mapping and mutant analysis, in unraveling the function of genes and their role in growth and development.Arabidopsis natural variation ͉ sucrose synthase ͉ hypocotyls T otal plant yield depends on the acquisition of raw material, i.e., photosynthesis and mineral (plus water) uptake, and on the ability of the plant to cope with stress. However, the economic yield of a crop is to a large extent also determined by the partitioning of dry matter over the harvestable and nonharvestable parts of the plant. The molecular and physiological basis of the regulation of assimilate partitioning in plants is still poorly understood. In terms of biomass, the most important components in assimilate partitioning and in total yield are carbohydrates. There is increasing evidence that a limited number of key enzymes, involved in primary (carbohydrate) metabolism, might be pivotal in this process (1).Functionally, a plant can be divided into sources (the sites of assimilate production) and sinks (the sites of use and͞or storage). Sinks can either be rapidly growing, expanding organs, such as elongating stems and roots, or storage sinks accumulating reserves, such as fruits, seeds, or tubers (2).In most plant species, carbon is transported from source to sink in the form of the disaccharide sucrose. Upon arrival in the sink, sucrose has to be hydrolyzed. In plants, two pathways are available for sucrose cleaving: via invertase (Inv), yielding glucose and fructose, and via sucrose synthase (Susy), yielding fructose and UDP-glucose. In several cases, it has been suggested that sink strength might depend on the activities of these sucrose-splitting enzymes. There is increasing evidence that in storage sinks, the predominant pathway is via Susy, whereas in growing sinks, the Inv route is most important. In potatoes, the elongating rhizomes (stolons) exhibit high Inv activity, whereas a switch toward Susycatalyzed sucrose bre...

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“…F2 and RIL populations derived from the crosses between Col-0 and Ler (Lister and Dean 1993) and between Cvi-1 and Ler (Alonso-Blanco et al 1998b) were obtained from the Nottingham Arabidopsis Stock Center (NASC) or from the Institute for Agronomy Research (INRA)-Versailles collection. Plants were grown in a BL-3 containment greenhouse under temperature-and humidity-controlled conditions (20°C and relative humidity of 60%).…”

Section: Plant Materialsmentioning

confidence: 99%

Identification of Quantitative Trait Loci Controlling Symptom Development During Viral Infection inArabidopsis thaliana

Sicard¹,

Keurentjes²,

Candresse³

et al. 2008

MPMI

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In compatible interactions between plants and viruses that result in systemic infection, symptom development is a major phenotypic trait. However, host determinants governing this trait are mostly unknown, and the mechanisms underlying it are still poorly understood. In a previous study on the Arabidopsis thaliana-Plum pox virus (PPV) pathosystem, we showed a large degree of variation in symptom development among susceptible accessions. Two basic types of interaction exist between a virus and its host plant: i) a compatible interaction associated with plant invasion and viral multiplication, followed in most cases by the induction of symptoms, and ii) an incompatible interaction with no or only limited viral multiplication or movement in the host plant. Plant-virus interactions are relatively well documented, but efforts have so far focused on resistance (or resistancebreaking) mechanisms and on the identification of their genetic determinants. Conversely, the one or more mechanisms controlling symptom development in plants are still largely unknown, so that only a very patchy picture of the interaction between a plant and a virus can be presented.

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Development of an AFLP based linkage map of Ler, Col and Cvi Arabidopsis thaliana ecotypes and construction of a Ler/Cvi recombinant inbred line population

Cited by 346 publications

References 47 publications

Gene Duplication in the Diversification of Secondary Metabolism: Tandem 2-Oxoglutarate–Dependent Dioxygenases Control Glucosinolate Biosynthesis in Arabidopsis

Gene Duplication in the Diversification of Secondary Metabolism: Tandem 2-Oxoglutarate–Dependent Dioxygenases Control Glucosinolate Biosynthesis in Arabidopsis

Vacuolar invertase regulates elongation of Arabidopsis thaliana roots as revealed by QTL and mutant analysis

Identification of Quantitative Trait Loci Controlling Symptom Development During Viral Infection inArabidopsis thaliana

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