Development of an Agrobacterium-mediated stable transformation method for industrial oilseed crop Crambe abyssinica ‘BelAnn’

Chhikara, Sudesh; Dutta, Indrajit; Paulose, Bibin; Jaiwal, Pawan K.; Dhankher, Om Parkash

doi:10.1016/j.indcrop.2011.07.021

Cited by 27 publications

(30 citation statements)

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“…For this stage, ½MS medium was first used with 0.5 µM ANA. Next, the shoots were transferred to ½MS medium with 1.0 µM ANA or 0.1 µM BAP, both supplemented with 10 g/L sucrose, 100 mg/L penicillin Chhikara et al, 2012), 0.5 mg/L AgNO 3 , and 7.5 g/L agar. The test was performed after 30 days in the ANA or BAP rooting medium as described, at 150 and 180 days, respectively.…”

Section: Culture Medium and Culturing Conditionsmentioning

confidence: 99%

“…Additionally, the agronomic characteristics of crambe, such as its short life cycle of approximately 90 days, high biomass, mean seed yield of 1800-2400 kg/ha, adaptation to different climatic conditions, resistance to insects, and tolerance to abiotic stresses such as salinity, low temperature, heat, drought, and exposure to heavy metals, make it an important species within the agricultural industry (Paulose et al, 2010;Chhikara et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

“…In this context, in vitro cell and tissue culturing techniques are important both for mass propagation Li et al, 2011;Palmer and Keller, 2011;Chhikara et al, 2012) and as a tool for genetic improvement (Wang et al, 2004;Wang et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

“…Micropropagation studies have documented crambe regeneration in vitro via direct Li et al, 2011) and indirect organogenesis (Chhikara et al, 2012), using hypocotyls as explants for both, and via indirect somatic embryogenesis (Palmer and Keller, 2011) with cotyledons, hypocotyls, and roots as explants. However, no published data describe crambe regeneration using apical segments via multiplication by axillary bud proliferation.…”

Section: Introductionmentioning

confidence: 99%

“…Crambe tissue culturing has been used to propagate (Li et al, 2011;Palmer and Keller, 2011), genetically improve Chhikara et al, 2012;Li et al, 2013), and to identify and use some of its genes to improve other species (Wang et al, 2004;Wang et al, 2006). The use of molecular markers in crambe reported in the literature is limited to the study of genetic diversity (Lara-Fioreze et al, 2013), identification and characterization of genetic transformants (Wang et al, 2004;Wang et al, 2006;Li et al, 2010;Xu et al, 2010;Chhikara et al, 2012;Li et al, 2013), and identification of genes of interest (Paulose et al, 2010;Cheng et al, 2013).…”

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confidence: 99%

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Genetic stability of micropropagated plants of Crambe abyssinica Hochst using ISSR markers

Werner¹,

Soares²,

Gontijo³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Crambe (Crambe abyssinica) is a non-edible annual herb, which was first cultivated to extract oil for industry, and now has great potential for biodiesel production. The objective of this investigation was to evaluate the genetic stability of micropropagated plants of the C. abyssinica Hochst cultivar 'FMS brilhante' using polymerase chain reaction techniques based on inter-simple sequence repeat (ISSR) molecular markers. The aim was to develop a protocol for the in vitro regeneration of these plants with low genetic variation as compared to the donor plant. For micropropagation, shoot tips from in vitro germinated seedlings were used as explants and were initially cultivated for 90 days on MS medium with 5.0 µM 6-benzylaminopurine (BAP), which at 90 days, led to the highest number of shoots per explant (NSE) (12.20 shoots) being detected. After 120 days, the interaction between BAP concentration and naphthalene acetic acid (NAA) was tested, and the highest NSE was observed following exposure to 0.0/0.5 µM BAP/NAA (11.40 shoots) and 1.0/0.0 µM BAP/NAA (11.00 shoots). The highest proportion of rooting phase were observed following exposure to 0.5 µM NAA (30%). The 13 ISSR primers used to analyze genetic stability produced 91 amplification products, of which only eight bands were polymorphic and 83 were monomorphic for all 10 regenerated crambe plants, compared to the donor plant explant. These results indicate that crambe shoot tips are a highly reliable explant that can be used to micropropagate genetically true-to-type plants or to maintain genetic stability, as verified using ISSR markers.

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Section: Culture Medium and Culturing Conditionsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%