Development of an Amperometric Ethanol Biosensor Based on a Multiwalled Carbon Nanotube-Nafion-Alcohol Dehydrogenase Nanobiocomposite

Liaw, Hong-Wei; Chen, Jieming; Tsai, Yu-Chen

doi:10.1166/jnn.2006.510

Cited by 25 publications

(19 citation statements)

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“…Enzymatic biofuel cells can also electrochemically detect a wide range of substrates from simple alcohols to macromolecules such as immunoglobulins. 25,[58][59][60][61] …”

Section: Analytical Applications Of Biofuel Cellsmentioning

confidence: 99%

Analytical Techniques for Characterizing Enzymatic Biofuel Cells

2009

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The rapid depletion in global nonrenewable energy stores has prompted a dramatic increase in both academic and industrial research toward alternate means of energy conversion. Although improbable as a single solution to the problem, fuel cells provide many potential contributions in the form of performance combined with scalability. Fuel cells have demonstrated high levels of power production through the consumption of renewable resources in an easily engineered small scale device that operates under mild conditions and could potentially replace today's ubiquitous batteries. Many evaluate energy conversion devices on the basis of the amount of energy converted per unit volume [energy density (Whr/L)] or per unit mass [specific energy (Whr/kg)]. Fuel cells not only rival battery performance in these terms, but also do not require time-consuming charging and do not suffer from the severe hysteresis effects seen in secondary batteries. However, many of these devices use expensive precious metal catalysts that often limit their commercial viability. Passivation by carbon monoxide or other byproducts causes losses in power production over time, and these devices often require high temperatures and harsh conditions to operate efficiently.Thus, many researchers have looked to nature to assist in our current energy conversion needs. Because of biological versatility and efficiency, organisms are able to convert enormous amounts of energy from an incomparable range of chemical substrates. Many researchers have examined ways of harnessing this ability using biofuel cells. Biofuel cells replace the metal catalyst with a biological catalyst: a microbe, enzyme, or even organelle interacting with an electrode surface. 1-3 These types of catalysts offer great benefits in catalytic activity, specificity, and cost. However, development and full evaluation of these dynamic and often sensitive bioelectrochemical systems require a diverse range of expertise. This article will focus on the current techniques used to evaluate the integrity, kinetics, and performance of enzymatic biofuel cells and the applications of the devices. The techniques described span not only the obvious electroanalytical characterization methods needed to evaluate a power source or analytical device, but also common biological and materials characterization techniques. Enzymatic biofuel cells are in an early stage of development, so new analytical techniques are being employed to understand the advantages and limitations of this technology and the engineering design envelope for applications. SPECTROSCOPIC TECHNIQUESEnzymatic biofuel cells employ oxidoreductase enzymes capable of catalyzing redox reactions. Enzymes that are free in solution ROBERT GATES Anal. Chem. 2009, 81, 9538-9545 10

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“…Enzymatic biofuel cells can also electrochemically detect a wide range of substrates from simple alcohols to macromolecules such as immunoglobulins. 25,[58][59][60][61] …”

Section: Analytical Applications Of Biofuel Cellsmentioning

confidence: 99%

Analytical Techniques for Characterizing Enzymatic Biofuel Cells

2009

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“…A lot of materials have been used to fabricate tyrosinase-based biosensor, for example, alumina sol-gel [15], polythiophene [16], graphite-Teflon composite [17], and chitosan [18]. Recently, the development in the field of MWNTNafion composite film applied to electroanalysis has opened a new range of possibilities for the construction of electrochemical biosensors based on these materials [19][20][21][22].…”

Section: Introductionmentioning

confidence: 99%

“…Depending on CNTs electrochemical properties, the CNTs-based sensors can be used for detecting NADH, hydrogen peroxide at a lower working potential [19,21,30]. Recent studies demonstrated improved electrochemical responses of ethanol and glucose determination at MWNT-Nafion nanocomposite film modified electrode [19,20].…”

Section: Introductionmentioning

confidence: 99%

Amperometric biosensors based on multiwalled carbon nanotube-Nafion-tyrosinase nanobiocomposites for the determination of phenolic compounds

Tsai

Chiu

2007

Sensors and Actuators B: Chemical

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166

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“…Many polymers have been reported for such applications, including nafion (Fortier et al, 1992;Vaillancourt et al, 1999) , polypyrrole (Branzoi & Pilan 2008;Ekanayake et al, 2007), polytyramine (Situmorang et al, 1999) and silicate sol-gels (Llaudet et al, 2005;Rickus et al, 2002;Salimi et al, 2004). Nafion is a negatively charged sulfonated tetrafluorethylene copolymer, which possesses a strong surface adhesion to electrode surface and a low swelling capability in aqueous media (Gong et al, 2005;Liaw et al, 2006;. Thus, nafion is quite appropriate for enzyme immobilization.…”

Section: Enzyme Immobilization Approachesmentioning

confidence: 99%