Development of an antibody ELISA for potency testing of furunculosis (Aeromonas salmonicida subsp salmonicida) vaccines in Atlantic salmon (Salmo salar L)

Positive correlation between Aeromonas salmonicida vaccine antigen concentration and protection in vaccinated rainbow trout Oncorhynchus mykiss evaluated by a tail fin infection model

“…Thus, survival rates were highest in fish injected with the highest antigen concentration (1 9 10 9 cfu mL fish À1 ), lower in fish immunized with 1 9 10 8 cfu mL fish À1 and lowest in fish immunized with 1 9 10 7 cfu mL fish À1 . The correlation in the present rainbow trout study with regard to protection and antigen concentration in the vaccines corresponds to previous studies performed on Atlantic salmon, Salmo salar (Linnaeus) (Bricknell et al 1999;Romstad et al 2012Romstad et al , 2013Romstad et al , 2014. The experimental vegetable oil was a suitable adjuvant when administered together with high concentrations of antigen as judged from the elicited antibody production and Figure 2 Levels of A. salmonicida-specific antibodies in serum were measured from 10 fish per group (5 fish per duplicate tank) by enzyme-linked immunosorbent assay.…”

“…; Romstad et al . , , ). The experimental vegetable oil was a suitable adjuvant when administered together with high concentrations of antigen as judged from the elicited antibody production and protection levels.…”

Vaccination of Atlantic lumpfish (Cyclopterus lumpus L.) at a low temperature leads to a low antibody response against Aeromonas salmonicida

“…Lumpfish vaccinated at a water temperature of 5°C did not demonstrate a significant antibody (IgM) response against A. salmonicida at any sampling point, when compared to control fish immunized with PBS. Our results are in agreement with other vaccination studies on Atlantic salmon, where fish reared and vaccinated at 2°C (Eggset et al., ) and 3°C (Romstad et al., ) showed very low antibody responses against A. salmonicida after injection. This is also supported by studies on other fish species immunized with different antigens at different water temperatures, where the lower temperature levels lead to either low or delayed specific immune responses (Lillehaug et al., ; Lorenzen et al., ; Martins et al., ; Rijkers, Frederixwolters, & Vanmuiswinkel, ; Xu, Sheng, Xing, & Zhan, ).…”

“…The lumpfish vaccinated at 15°C reached a peak in the antibody response against A. salmonicida at 6 weeks post‐immunization, while lumpfish vaccinated at 10°C reached the highest antibody response levels at eighteen weeks post‐immunization. Similar kinetics in the antibody response against A. salmonicida have been reported from studies on Atlantic salmon (Eggset, Mikkelsen, & Killie, ; Romstad, Reitan, Midtlyng, Gravningen, & Evensen, ). However, the duration of high antibody levels was different, where Romstad et al.…”

“…However, the duration of high antibody levels was different, where Romstad et al. () observed a significant decrease from 9 wpi to 12 wpi, and Eggset et al. () noted high levels at both 9 and 18 wpi.…”