Development of an Approach of High Sensitive Chemiluminescent Assay for Cystatin C Using a Nanoparticle Carrier

Abstract

Cystatin C is an important cysteine protease inhibitor in the human body and is proposed as a new indicator of glomerular filtration rate for the detection of kidney damage. In this article, we report an ultra-sensitive, simple, and rapid chemiluminescence immunoassay method for cystatin C detection using functionalized mesoporous silica nanoparticles. After a three step hydrolysis, the amino-functionalized MSN encapsulating dye resulted in a hydrophobic environment for fixing the dye and amino groups for biological modification. The NaIO 4 immobilization method maintained the activity of the antibody notably well. The sandwich immunoassay using two monoclonal antibodies was chosen for its selectivity. The analysis demonstrated that the detection upper was 0.0029 ng/mL and linear relationship within the range of 0.0035–0.5 ng/mL ( R 2 = 0.9936). The relative standard deviation (RSD) for 11 parallel measurements of 0.25 ng/mL CysC was 4.7%. The automated chemiluminescence analyzer could detect 96 wells continuously. The results demonstrated that this method is ultra-sensitive, simple, and rapid for detecting cystatin C.

Preparation of PVA/PEI/CNC/ZnO composite membrane with good mechanical properties and water resistance by electrostatic spinning using for efficient filtration of PM2.5

“…To improve the water resistance of the system, MTMS was introduced and provided the low surface free energy by hydrolyzing condensation to form long hydrophobic chains attached to the surface of the membrane. Therefore, the air filtration membranes with high efficiency filtration, good mechanical strength and water resistance were prepared 23 …”

“…Therefore, the air filtration membranes with high efficiency filtration, good mechanical strength and water resistance were prepared. 23…”

Advancements in nanomedicines for the detection and treatment of diabetic kidney disease

“…With this in mind, Sun et al. developed a sandwich chemiluminescence immunodetection method for cystatin C labelled with amino-functionalised mesoporous silica NPs encapsulating dye [ 137 ]. Compared with ELISA this NP based approach demonstrated considerably greater sensitivity (limit of detection 0.0333 ng/mL vs 0.0029 ng/mL, respectively).…”