Development of an Array of Ion-Selective Microelectrodes Aimed for the Monitoring of Extracellular Ionic Activities

Guénat, O.; Generelli, Silvia; Rooij, N. F. de; Koudelka‐Hep, M.; Berthiaume, François; Yarmush, Martin L.

doi:10.1021/ac0609733

Cited by 31 publications

(27 citation statements)

References 35 publications

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“…A typical example of ISE is the glass electrode used for measuring the pH value. Moreover, ISEs for ions such as ammonium, potassium, and calcium are of great interest in applications like water quality control [5] and biomedical monitoring [6], [7].…”

Section: Introductionmentioning

confidence: 99%

A Bayesian Nonlinear Source Separation Method for Smart Ion-Selective Electrode Arrays

2009

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Abstract-Potentiometry with ion-selective electrodes (ISEs) provides a simple and cheap approach for estimating ionic activities. However, a well-known shortcoming of ISEs regards their lack of selectivity. Recent works have suggested that smart sensor arrays equipped with a blind source separation (BSS) algorithm offer a promising solution to the interference problem. In fact, the use of blind methods eases the time-demanding calibration stages needed in the typical approaches. In this work, we develop a Bayesian source separation method for processing the outputs of an ISE array. The major benefit brought by the Bayesian framework is the possibility of taking into account some prior information, which can result in more realistic solutions. Concerning the inference stage, it is conducted by means of Markov chain Monte Carlo (MCMC) methods. The validity of our approach is supported by experiments with artificial data and also in a scenario with real data.

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Section: Introductionmentioning

confidence: 99%

A Bayesian Nonlinear Source Separation Method for Smart Ion-Selective Electrode Arrays

2009

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“…This suggests that the relevant electrical specifi cations of the measurement system should be adjusted for cell culture applications. An example is that the ISE pH sensor exposed in culture media displayed a linear but subNernstrian response [24,25] . The calibration process reveals a 30 -mV/pH -unit slope in cell culture for pH measurement rather than a 59 -mV/pH -unit slope, which is calculated via Equation 11.2 .…”

Section: Sensors and Bioreactorsmentioning

confidence: 99%

Monitoring of Stem Cell Culture Process Using Electrochemical Biosensors

Iniewski

2011

CMOS Biomicrosystems

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The fundamental bottleneck of any bioprocess is the lack of real -time, online, in situ , quantitative information with respect to cellular behaviors in cultures. As a result, control, optimization, and scale -up of bioprocesses are essentially manual (empirical) -which results in suboptimal productivity (i.e., inadequate cell expansion) and product quality. To harness the immense potential of stem cells in terms of their plasticity and expansion capabilities, the physiological activity in relation to local culture parameters, such as pH, dissolved oxygen, nutrient and metabolite concentrations, and growth factor concentrations, needs to be recorded quantitatively with the required level of accuracy. Subsequently, it must be evaluated in a biologically meaningful manner, which, in the long run, could allow the systematic development of clinically relevant culture systems and methodologies, leading to the engineering of reproducible, well -characterized, regenerated " designer " tissues and organs that meet the strict regulatory criteria for clinical applications.This chapter will discuss the acquirement of quantitative information of the cell culture bioprocess using biosensors. The interfacing for a single potentiometric biosensor and for a single amperometric biosensor will be discussed fi rst, and then followed by the discussion on building a multichannel, PC -based, real -time measurement system. An up to 128 -channel, multiparametric physiological measurement system for monitoring stem cell culture process will be described in detail as an example.

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“…Arrays of 24 or 16 Ca 2+ [37], K + , and NH 4 + micropipette electrodes were constructed for monitoring extracellular ion activities in cell cultures. Due to the rather large internal membrane reservoir, excellent lifetimes (>1 month) have been achieved [38].…”

Section: Ion-selective Microelectrodes and Nanoelectrodesmentioning

confidence: 99%

Nanoscale potentiometry

Bakker

Pretsch²

2008

TrAC Trends in Analytical Chemistry

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Potentiometric sensors share unique characteristics that set them apart from other electrochemical sensors. Potentiometric nanoelectrodes have been reported and successfully used for many decades, and we review these developments. Current research chiefly focuses on nanoscale films at the outer or the inner side of the membrane, with outer layers for increasing biocompatibility, expanding the sensor response, or improving the limit of detection (LOD). Inner layers are mainly used for stabilizing the response and eliminating inner aqueous contacts or undesired nanoscale layers of water. We also discuss the ultimate detectability of ions with such sensors and the power of coupling the ultra-low LODs of ion-selective electrodes with nanoparticle labels to give attractive bioassays that can compete with state-of-the-art electrochemical detection.

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Development of an Array of Ion-Selective Microelectrodes Aimed for the Monitoring of Extracellular Ionic Activities

Cited by 31 publications

References 35 publications

A Bayesian Nonlinear Source Separation Method for Smart Ion-Selective Electrode Arrays

A Bayesian Nonlinear Source Separation Method for Smart Ion-Selective Electrode Arrays

Monitoring of Stem Cell Culture Process Using Electrochemical Biosensors

Nanoscale potentiometry

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