Development of an automated plant culture system

Tisserat, Brent; Vandercook, Carl E.

doi:10.1007/bf00040307

Cited by 83 publications

(31 citation statements)

References 3 publications

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“…Similar to the current observation the positive influence of liquid medium on fresh mass development was reported for African oil palm (Teixeira et al, 1995;Kanchanapoom and Chourykaew, 1998) and date palm (Tisserat and Vandercook, 1985;Al-Khayri, 2012). A higher mass of African oil palm callus as compared to solidified medium was also reported using TIS (Sumaryono et al, 2008).…”

Section: Results and Discussion Experiments 1: The Effect Of Culture Ssupporting

confidence: 90%

“…Adding BAP to the cell suspension cultures seemed to be controversial. Using liquid medium with NAA and without cytokinins as we found, was reported to be optimal in some date palm liquid cultures (Tisserat and Vandercook, 1985;Sharma et al, 1986;Saker et al, 2007). In contrast to our results, Gadallah (2007) reported higher fresh mass and somatic embryo number in date palm cv.…”

Section: Callus Induced From Shoot Tipssupporting

confidence: 55%

“…To our knowledge there is no optimal protocol for using RITA ® system for date palm tissue cultures. Tisserat and Vandercook (1985) reported developing an automated plant culture system (APCS) for date palm callus with 5-10 min immersion every 2 h. They found superior growth of callus which reached up to a 4-fold increase as compared to those grown on agar medium. In our experiment callus increased only 1.5-2 fold compared to agar-medium (Table 1).…”

Section: Results and Discussion Experiments 1: The Effect Of Culture Smentioning

confidence: 99%

“…To meet the increasing demand for date palms, Okere et al (2010) recommended complementing the tissue culture techniques with temporary immersion bioreactor systems (TIBs) to enhance the commercial production of date palm plantlets. Tisserat and Vandercook (1985) developed an automated plant culture system and investigated it on some plant species and explants and they included date palm callus in their study. Their results showed better callus growth on this system compared to agar medium.…”

Section: Introductionmentioning

confidence: 99%

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A comparative study between solid and liquid cultures relative to callus growth<br>and somatic embryo formation in date palm (Phoenix dactylifera L.) cv. Zaghlool

Ibraheem

Pinker

Böhme

2013

Emir. J. Food Agric

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Tissue culture techniques enable mass propagation of elite cultivars of date palm (Phoenix dactylifera L.). The main limitations for date palm in vitro multiplication are the low rates achieved using solidified medium and the long period needed to produce acclimatized plantlets. This research focuses on the comparison between different culture types and plant growth regulator (PGR) combinations on callus growth and somatic embryo formation of cv. Zaghlool. For callus growth, 200 mg friable embryogenic callus dispensed in Rasotherm and Phytacon flasks containing 200 ml liquid (100 rotations per minute) and in temporary immersion system, RITA ® bioreactor (5 min immersion every 12 h), were compared with cultures grown on 200 ml solidified medium. For somatic embryo formation, 500 mg of friable embryogenic callus grown in Erlenmeyer flasks filled with 50 ml liquid or solid MS medium. The medium was supplemented with 0.1 mg l -1 Naphthaleneacetic acid (NAA), 1.5 g l -1 activated charcoal (AC) with or without 0.05 mg l -1 6-Benzyl amino purine (BAP) compared to PGR-free medium. Results proved that cell suspension cultures produced the highest callus fresh mass as compared to the other systems tested, and the callus fresh mass reached 4 g after 16 weeks. The temporary immersion system did not significantly enhance the fresh mass of callus compared to the solidified medium. For somatic embryo induction, the number of somatic embryos increased in cell suspensions 6-16 fold compared to the solidified medium. Using liquid MS medium enriched with 0.1 mg l -1 NAA and 1.5 g l -1 AC gave rise to the highest number of somatic embryos formed from 500 mg initial callus: 160 embryos. The number of somatic embryos was also affected by the callus source. The calli induced from leaflet segments excised from converted somatic embryos resulted in a lower somatic embryo number than those of shoot tip origin with about 60 somatic embryos per 500 mg callus. The formation of somatic embryos using liquid medium required only 6 weeks, thus considerably reducing the previously reported period of 18 weeks which is required for somatic embryo formation using solidified media.

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Section: Results and Discussion Experiments 1: The Effect Of Culture Ssupporting

confidence: 90%

Section: Callus Induced From Shoot Tipssupporting

confidence: 55%

Section: Results and Discussion Experiments 1: The Effect Of Culture Smentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A comparative study between solid and liquid cultures relative to callus growth<br>and somatic embryo formation in date palm (Phoenix dactylifera L.) cv. Zaghlool

Ibraheem

Pinker

Böhme

2013

Emir. J. Food Agric

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“…Taking into account the liquid condition of the medium, a system was initially designed that consisted of a large, elevated culture chamber that could be drained and then refilled with fresh medium (Tisserat and Vandercook, 1985). Subsequently a semi-automated system was developed in which plants were cultured in a large container on a medium that contained no gelling agent, with automatic addition and removal of liquid medium at fixed intervals so the plant material entered into contact with the medium several times throughout the day (Aitken-Christie and Davies, 1988).…”

Section: Introductionmentioning

confidence: 99%

Micropropagation of Guadua angustifolia Kunth (Poaceae) using a temporary immersion system RITA

GutiÃ©rrez¹,

Lopez-Franco²,

Morales-PinzÃ3n³

2016

Afr. J. Biotechnol.

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The micropropagation of Guadua angustifolia Kunth, commonly known as giant bamboo, using semisolid culture medium, is reported to have low multiplication rates. This study evaluated the multiplication index of G. angustifolia in a temporary immersion system (RITA ® ), comparing results with those obtained using a semi-solid culture medium. The treatments consisted of either three or four 2-min immersions per day and use of semi-solid culture medium, which consisted of MS supplemented with 3.0 mg L -1 of the cytokinin benzylaminopurine (BAP). Equipment consisted of 20 vessels for automated RITA ® , each containing 200 ml of culture medium. Immersions were performed for 2 min at two different frequency intervals (6 and 8 h). Large clumps of G. angustifolia with 1, 2 or 3 stems were inoculated depending on the treatment. Best results were obtained with four immersion cycles per day (every 6 h), with a multiplication index of 2.7 shoots per original explant (axillary buds) and greater rhizome growth. Overall, the temporary immersion system performed better than the semi-solid medium in terms of shoot multiplication rates and rhizome growth. Further studies should be conducted to develop an application for RITA ® for use in the commercial production of G. angustifolia.

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Controlling hyperhydration of carnations (Dianthus caryophyllus L.) grown in a mist reactor

Correll

Weathers

2000

Biotechnol. Bioeng.

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Development of an automated plant culture system

Cited by 83 publications

References 3 publications

A comparative study between solid and liquid cultures relative to callus growth<br>and somatic embryo formation in date palm (Phoenix dactylifera L.) cv. Zaghlool

A comparative study between solid and liquid cultures relative to callus growth<br>and somatic embryo formation in date palm (Phoenix dactylifera L.) cv. Zaghlool

Micropropagation of Guadua angustifolia Kunth (Poaceae) using a temporary immersion system RITA

Controlling hyperhydration of carnations (Dianthus caryophyllus L.) grown in a mist reactor

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