ABSTRACT. The performance of the CO 2 -accepting SO-110 ion source at Oxford Radiocarbon Accelerator Unit has been investigated in detail. The purpose was to clarify the possibilities of accelerator mass spectrometry (AMS) radiocarbon measurements with real-time separation, e.g. GC AMS or HPLC AMS. The construction of a gas test injector based on the continuous-flow technique made it possible to characterize the response of the ion source to continuous and pulsed input of CO 2 gas. The source exhibited remarkably good linearity over a wide range of CO 2 -pulse sizes and fast rise time, but the peak shape varied and memory effects were significant. Appropriate tuning of the gas source proved to be critical.