Development of an efficient real-time PCR assay to quantify enterotoxin-producing staphylococci in meat products

Rodríguez, Alicia; Gordillo, Rubén; Andrade, María J.; Córdoba, Juan J.; Rodrı́guez, Mar

doi:10.1016/j.foodcont.2015.07.040

Cited by 24 publications

(17 citation statements)

References 44 publications

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“…1 fg of purified target DNA in dried skimmed milk. Currently, several PCR variants have been developed to detect SEs, such as multiplex PCR [ 29 , 30 ], real-time PCR [ 31 , 32 ], reverse-transcriptase PCR (RT-PCR) [ 33 , 34 ], and loop-mediated isothermal amplification (LAMP) [ 35 , 36 , 37 ]. Compared to the other PCR-based techniques, the distinct advantage of multiplex PCR is simultaneous detection of several SEs with different primers.…”

Section: Se Identification Using Molecular Biological Methodsmentioning

confidence: 99%

A Review of the Methods for Detection of Staphylococcus aureus Enterotoxins

Duan

et al. 2016

Toxins

137

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Food safety has attracted extensive attention around the world, and food-borne diseases have become one of the major threats to health. Staphylococcus aureus is a major food-borne pathogen worldwide and a frequent contaminant of foodstuffs. Staphylococcal enterotoxins (SEs) produced by some S. aureus strains will lead to staphylococcal food poisoning (SFP) outbreaks. The most common symptoms caused by ingestion of SEs within food are nausea, vomiting, diarrhea and cramps. Children will suffer SFP by ingesting as little as 100 ng of SEs, and only a few micrograms of SEs are enough to cause SPF in vulnerable populations. Therefore, it is a great challenge and of urgent need to detect and identify SEs rapidly and accurately for governmental and non-governmental agencies, including the military, public health departments, and health care facilities. Herein, an overview of SE detection has been provided through a comprehensive literature survey.

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Section: Se Identification Using Molecular Biological Methodsmentioning

confidence: 99%

A Review of the Methods for Detection of Staphylococcus aureus Enterotoxins

Duan

et al. 2016

Toxins

137

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“…Hiện nay có thêm nhiều phương pháp phát hiện SEB và các gen SE khác được phát triển từ kỹ thuật PCR như PCR đa mồi (Multiplex PCR) (Naresh et al, 2000;Shylaja et al, 2010); PCR định lượng theo thời gian (real-time PCR) (Letertre et al, 2003;Rodríguez et al, 2016); PCR phiên mã ngược (reverse-transcriptase PCR hay RT-PCR) (Matsui et al, 1997). So sánh với các phương pháp dựa trên kỹ thuật PCR cơ bản thì phương pháp PCR đa mồi có lợi thế hơn cả vì nó có thể phát hiện đồng thời các gen độc tố SE trong cùng một phản ứng và xác định đúng 99% tương ứng với các loại độc tố được xác định theo tiêu chuẩn kiểm tra miễn dịch (Naresh et al, 2000), nhưng kỹ thuật này không cơ động, khá phức tạp và đòi hỏi cán bộ nghiên cứu có chuyên môn, kỹ thuật.…”

Section: Kỹ Thuật Sinh Học Phân Tửunclassified

Staphylococcal enterotoxin B (SEB) toxin of Staphylococcus aureus and the detection methods

Thu

Minh

2018

Vietnam J. Biotechnol.

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Staphylococcal enterotoxins (SEs) secreted by Staphylococcus aureus is one of the principal causes of food poisoning. The SEs are superantigens; they are highly stable, resisting most proteolytic enzymes and thus keeping activity in the gastrointestinal tract after being ingestion. In particular, heat-stable enterotoxin is one of the most important property related to food safety. They are not degraded at 100°C for 30 minutes, even at 121oC for 28 minutes, the SEs retain biological activity. Heat resistance of SEs in foods is higher than in the culture medium. Staphylococcus aureus (S. aureus) produces more than 20 different types of enterotoxins, including SEA to SEE, SEG to SER and SEU. Among these, Staphylococcal enterotoxin B (SEB) is a powerful toxin, heat-stable, water-soluble and is a common cause of food poisoning. Moreover, SEB is one of the harmful or hazardous agents used as biological weapons in bioterrorism or biological warfare. Therefore, determining presence of SEB toxin in food is extremely important. In this review, we introduce the most basic features about S. aureus; about SEB toxin and conventional methods for SEB diagnosis, detection. Especially, we focus on rapid detection strip based on an immunochromatography; this technique is an highly sensitive, rapid, easy for use and storage.

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“…Similar to other rapid detection tests such as coagulase, biochemical and immunological, MNase-based assays were shown to have variable sensitivity and reliability when applied to direct blood samples (Cao et al 2009). PCR-based methods have been developed for S. aureus detection in food and clinical samples (Rodriguez et al 2016;Wang 2016). Although PCR-based sensing assays significantly decrease time to results, they are still labour intensive and require sophisticated instrumentation with trained personnel.…”

Section: Introductionmentioning

confidence: 99%