Development of an Electrochemical Biosensor for the Rapid Detection of Cholera Toxin Based on Air Stable Lipid Films with Incorporated Ganglioside GM1 Using Graphene Electrodes

Karapetis, Stephanos; Nikoleli, Georgia‐Paraskevi; Siontorou, Christina G.; Nikolelis, Dimitrios P.; Tzamtzis, Nikolaos; Psaroudakis, Nikolas

doi:10.1002/elan.201501134

Cited by 36 publications

(30 citation statements)

References 39 publications

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“…Our group has constructed a device that was composed from a stabilized lipid membrane on a copper wire that contained graphene nanosheets [69,70]. These nanodevices have been used for the rapid determination of food toxicants, environmental pollutants, and compounds of clinical interest [71][72][73].…”

Section: Polymeric Lipid Membranes Supported On Graphene Microelectrodesmentioning

confidence: 99%

Recent Lipid Membrane-Based Biosensing Platforms

et al. 2019

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The investigation of lipid films for the construction of biosensors has recently given the opportunity to manufacture devices to selectively detect a wide range of food toxicants, environmental pollutants, and compounds of clinical interest. Biosensor miniaturization using nanotechnological tools has provided novel routes to immobilize various “receptors” within the lipid film. This chapter reviews and exploits platforms in biosensors based on lipid membrane technology that are used in food, environmental, and clinical chemistry to detect various toxicants. Examples of applications are described with an emphasis on novel systems, new sensing techniques, and nanotechnology-based transduction schemes. The compounds that can be monitored are insecticides, pesticides, herbicides, metals, toxins, antibiotics, microorganisms, hormones, dioxins, etc.

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Section: Polymeric Lipid Membranes Supported On Graphene Microelectrodesmentioning

confidence: 99%

Recent Lipid Membrane-Based Biosensing Platforms

et al. 2019

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“…The as-prepared biosensor allowed the detection to be performed over a wide range of toxin concentrations, providing a fast response time of ca. 5 min, and an LOD of 1 nM [138]. Finally, as an example of a toxin from vegetal origins, it is worth mentioning ricin, from the plant Ricinus communis, which is a cytotoxic protein that is considered to be a potential threat agent for terrorist use because of its high toxicity, absence of curative treatments, and ease of manufacture.…”

Section: Biological Weaponsmentioning

confidence: 99%

What Electrochemical Biosensors Can Do for Forensic Science? Unique Features and Applications

et al. 2019

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This article critically discusses the latest advances in the use of voltammetric, amperometric, potentiometric, and impedimetric biosensors for forensic analysis. Highlighted examples that show the advantages of these tools to develop methods capable of detecting very small concentrations of analytes and provide selective determinations through analytical responses, without significant interferences from other components of the samples, are presented and discussed, thus stressing the great versatility and utility of electrochemical biosensors in this growing research field. To illustrate this, the determination of substances with forensic relevance by using electrochemical biosensors reported in the last five years (2015–2019) are reviewed. The different configurations of enzyme or affinity biosensors used to solve analytical problems related to forensic practice, with special attention to applications in complex samples, are considered. Main prospects, challenges to focus, such as the fabrication of devices for rapid analysis of target analytes directly on-site at the crime scene, or their widespread use and successful applications to complex samples of interest in forensic analysis, and future efforts, are also briefly discussed.

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“…A potentiometric miniature cholera toxin device which was based on a graphene electrode with deposited lipid membranes has appeared in the literature [14]. Ganglioside GM1 (which is the natural receptor for cholera toxin) was mixed with the lipid mixture prior to polymerization.…”

Section: Potentiometric Applications On Biosensors Based On Lipid Memmentioning

confidence: 99%

Potentiometric Biosensing Applications of Graphene Electrode with Stabilized Polymer Lipid Membranes

Nikoleli¹,

Nikolelis²,

Siontorou³

et al. 2018

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This review provides informations and details for the fabrication of biosensors that are composed from lipid membranes and have been utilized and applied to rapidly detect food toxic compounds, environmental pollutants and analytes of clinical interest. Biosensors based on polymeric lipid membranes have been used to rapidly detect a wide range of these analytes and offer several advantages such as fast response, high sensitivity and selectivity, can be portable for in the field applications, and small size. A description of the construction of these devices and their applications for the rapid detection of food toxic substance, environmental pollutants and analytes of clinical interest is provided in this review.

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Development of an Electrochemical Biosensor for the Rapid Detection of Cholera Toxin Based on Air Stable Lipid Films with Incorporated Ganglioside GM1 Using Graphene Electrodes

Cited by 36 publications

References 39 publications

Recent Lipid Membrane-Based Biosensing Platforms

Recent Lipid Membrane-Based Biosensing Platforms

What Electrochemical Biosensors Can Do for Forensic Science? Unique Features and Applications

Potentiometric Biosensing Applications of Graphene Electrode with Stabilized Polymer Lipid Membranes

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