Development of an Electrochemical Biosensor for the Rapid Detection of Saxitoxin Based on Air Stable Lipid Films with Incorporated Anti‐STX Using Graphene Electrodes

Bratakou, Spyridoula; Nikoleli, Georgia‐Paraskevi; Siontorou, Christina G.; Nikolelis, Dimitrios P.; Karapetis, Stephanos; Tzamtzis, Nikolaos

doi:10.1002/elan.201600652

Cited by 59 publications

(41 citation statements)

References 43 publications

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“…A miniaturized potentiometric saxitoxin immunosensor was reported, involving graphene nanosheets with incorporated lipid films and immobilized anti-STX. The achieved LOD was 1 nM, and the method was tested in lake water and shellfish samples [87].…”

Section: Toxinsmentioning

confidence: 99%

What Electrochemical Biosensors Can Do for Forensic Science? Unique Features and Applications

et al. 2019

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This article critically discusses the latest advances in the use of voltammetric, amperometric, potentiometric, and impedimetric biosensors for forensic analysis. Highlighted examples that show the advantages of these tools to develop methods capable of detecting very small concentrations of analytes and provide selective determinations through analytical responses, without significant interferences from other components of the samples, are presented and discussed, thus stressing the great versatility and utility of electrochemical biosensors in this growing research field. To illustrate this, the determination of substances with forensic relevance by using electrochemical biosensors reported in the last five years (2015–2019) are reviewed. The different configurations of enzyme or affinity biosensors used to solve analytical problems related to forensic practice, with special attention to applications in complex samples, are considered. Main prospects, challenges to focus, such as the fabrication of devices for rapid analysis of target analytes directly on-site at the crime scene, or their widespread use and successful applications to complex samples of interest in forensic analysis, and future efforts, are also briefly discussed.

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Section: Toxinsmentioning

confidence: 99%

What Electrochemical Biosensors Can Do for Forensic Science? Unique Features and Applications

et al. 2019

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“…A paper was reported in the literature using a synthetic "receptor" A potentiometric saxitoxin minisensor based on graphene nanosheets with incorporated lipid films and immobilized anti-STX (which is the natural saxitoxin receptor) on stabilized lipid films was recently reported in the literature [32]. A good selectivity and sensitivity for the detection of saxitoxin, fast response times of ca.…”

Section: Applications Of Lipid Film Based Biosensors In Food Analysismentioning

confidence: 99%

Lipid Membrane Based Biosensors

Nikoleli¹,

Nikolelis²,

Siontorou³

et al. 2018

Preprint

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The exploitation of lipid membranes in biosensors has provided the ability to reconstitute a considerable part of their functionality to detect trace of food toxicants and environmental pollutants. Nanotechnology enabled sensor miniaturization and extended the range of biological moieties that could be immobilized within a lipid bilayer device. This chapter reviews recent progress in biosensor technologies based on lipid membranes suitable for environmental applications and food quality monitoring. Numerous biosensing applications are presented, putting emphasis on novel systems, new sensing techniques and nanotechnology-based transduction schemes. The range of analytes that can be currently detected include, insecticides, pesticides, herbicides, metals, toxins, antibiotics, microorganisms, hormones, dioxins, etc. Technology limitations and future prospects are discussed, focused on the evaluation/ validation and eventually commercialization of the proposed sensors.

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“…In one of our papers, electron scanning microscopy (SEM) experiments were performed in order to investigate the mechanism of signal generation [20]. The results have shown that the structure of the polymerized polyacrylate without the lipid has an amorphous structure, but when the lipid is copolymerized, the structure becomes crystalline.…”

Section: Mechanism Of Signal Generationmentioning

confidence: 99%

“…A miniaturized potentiometric saxitoxin sensor on graphene nanosheets with incorporated lipid films and Anti-STX, the natural saxitoxin receptor, immobilized on the stabilized lipid films is described in one of our recent papers [20]. An adequate selectivity for detection over a wide range of toxin concentrations, fast response time of ca.…”

Section: Examples Of Biosensors Based On Lipid Modified Graphene Micrmentioning

confidence: 99%

Biosensors Based on Lipid Modified Graphene Microelectrodes

Nikoleli

Siontorou

Nikolelis

et al. 2017

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Graphene is one of the new materials which has shown a large impact on the electronic industry due to its versatile properties, such as high specific surface area, high electrical conductivity, chemical stability, and large spectrum of electrochemical properties. The graphene material-based electronic industry has provided flexible devices which are inexpensive, simple and low power-consuming sensor tools, therefore opening an outstanding new door in the field of portable electronic devices. All these attractive advantages of graphene give a platform for the development of a new generation of devices in both food and environmental applications. Lipid-based sensors have proven to be a good route to the construction of novel devices with improved characteristics, such as fast response times, increased sensitivity and selectivity, and the possibility of miniaturization for the construction of portable biosensors. Therefore, the incorporation of a lipid substrate on graphene electrodes has provided a route to the construction of a highly sensitive and selective class of biosensors with fast response times and portability of field applications for the rapid detection of toxicants in the environment and food products.

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Development of an Electrochemical Biosensor for the Rapid Detection of Saxitoxin Based on Air Stable Lipid Films with Incorporated Anti‐STX Using Graphene Electrodes

Cited by 59 publications

References 43 publications

What Electrochemical Biosensors Can Do for Forensic Science? Unique Features and Applications

What Electrochemical Biosensors Can Do for Forensic Science? Unique Features and Applications

Lipid Membrane Based Biosensors

Biosensors Based on Lipid Modified Graphene Microelectrodes

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