Development of an electrospun poly(ε-caprolactone)/collagen-based human amniotic membrane powder scaffold for culturing retinal pigment epithelial cells

Majidnia, Elahe; Ahmadian, Mehdi; Salehi, Hossein; Amirpour, Noushin

doi:10.1038/s41598-022-09957-5

Cited by 12 publications

(21 citation statements)

References 65 publications

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“…The amide groups' presence in FTIR spectra of PCL‐AM scaffolds proved that AM was present in these hybrid nanofibers. Notably, the relative intensities of amide peaks increased with enhancing the AM content, which was in agreement with the investigation conducted by Majidnia et al 68 …”

Section: Resultssupporting

confidence: 92%

“…Favorably, no beads could be seen in various compositions of hybrid fibers (Figure 3). These results were analogous to the recent work of a group of scientists 68 . The mean diameters of PCL and PCL‐10AM nanofibers were 871.50 ± 118.59 and 759.00 ± 90.38 nm, respectively (Figure 4).…”

Section: Resultssupporting

confidence: 90%

“…All plots had two distinct areas: the initial linear region associated with the reversible elastic deformation and a non-linear upward stress part related to the irreversible plastic deformation. 74 Consistent with the previous reports, 68,79 the AM introduction increased the elastic modulus of fibers, possibly owing to the newer hydrogen bonds' formation between the AM's amide groups and PCL's carboxyl groups. Furthermore, thinner fibers have been shown to have higher Young modulus than the thicker ones, 80 which could be also seen in our experiment (Table 3).…”

Section: Mechanical Characteristics Assessmentsupporting

confidence: 88%

“…These results were analogous to the recent work of a group of scientists. 68 The mean diameters of PCL and PCL-10AM…”

Section: Morphological Characterizationmentioning

confidence: 99%

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Fabrication and multiscale modeling of polycaprolactone/amniotic membrane electrospun nanofiber scaffolds for wound healing

et al. 2023

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BackgroundEnhancing the efficiency of cell‐based skin tissue engineering (TE) approaches is possible via designing electrospun scaffolds possessing natural materials like amniotic membrane (AM) with wound healing characteristics. Concentrating on this aim, we fabricated innovative polycaprolactone (PCL)/AM scaffolds through the electrospinning process.MethodsThe manufactured structures were characterized by employing scanning electron microscope (SEM), attenuated total reflection‐Fourier transform infrared (ATR‐FTIR) spectroscopy, tensile testing, Bradford protein assay, etc. In addition, the mechanical properties of scaffolds were simulated by the multiscale modeling method.ResultsAs a result of conducting various tests, it was concluded that the uniformity and distribution of fibers decreased with an increase in the amniotic content. Furthermore, PCL‐AM scaffolds contained amniotic and PCL characteristic bands. In the case of protein release, greater content of AM led to the release of higher amounts of collagen. Tensile testing revealed that scaffolds' ultimate strength increased when the AM content augmented. The multiscale modeling demonstrated that the scaffold had elastoplastic behavior. In order to assess cellular attachment, viability, and differentiation, human adipose‐derived stem cells (ASCs) were seeded on the scaffolds. In this regard, SEM and 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyl‐2H‐tetrazolium bromide (MTT) assays showed significant cellular proliferation and viability on the proposed scaffolds, and these analyses illustrated that higher cell survival and adhesion could be achieved when scaffolds possessed a larger amount of AM. After 21 days of cultivation, particular keratinocyte markers, such as keratin I and involucrin, were identified through utilizing immunofluorescence and real‐time polymerase chain reaction (PCR) tests. The markers' expressions were higher in the PCL‐AM scaffold with a ratio of 90:10 v v−1 compared with the PCL‐epidermal growth factor (EGF) structure. Moreover, the presence of AM in the scaffolds resulted in the keratinogenic differentiation of ASCs even without employing EGF. Consequently, this state‐of‐the‐art experiment suggests that the PCL‐AM scaffold can be a promising candidate in skin bioengineering.ConclusionThis study showed that mixing AM with PCL, a widely used polymer, in different concentrations can overcome PCL disadvantages such as high hydrophobicity and low cellular compatibility.

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Section: Resultssupporting

confidence: 92%

Section: Resultssupporting

confidence: 90%

Section: Mechanical Characteristics Assessmentsupporting

confidence: 88%

“…These results were analogous to the recent work of a group of scientists. 68 The mean diameters of PCL and PCL-10AM…”

Section: Morphological Characterizationmentioning

confidence: 99%

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Fabrication and multiscale modeling of polycaprolactone/amniotic membrane electrospun nanofiber scaffolds for wound healing

et al. 2023

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“…Fibrous Membranes. Te electrospinning solution was prepared similarly to the optimum solution in reference [13]. Briefy, after receiving the human amniotic membranes, a 5% (wt/v) HAMP stock solution in a mixture of FA : AA with a volume ratio of 70 : 30 was prepared as described elsewhere.…”

Section: Electrospinning Of Random and Aligned Pcl-hampmentioning

confidence: 99%

The Effect of Aligned and Random PCL-Human Amniotic Membrane Powder Scaffolds on Retinal Tissue Engineering

Majidnia

Amirpour

Ahmadian

et al. 2023

Advances in Materials Science and Engineering

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One promising treatment for degenerative retinal diseases such as age-related macular degeneration (AMD) is the delivery of retinal pigment epithelial (RPE) cells using degradable scaffolds. Tough-aligned scaffolds are promising candidates for some applications of tissue engineering, such as peripheral nerve regeneration. However, aligned scaffolds have not been investigated in retinal tissue engineering so far. Here, a comparison was made between aligned and random scaffolds fabricated from polycaprolactone (PCL) and human amniotic membrane powder (HAMP) as a scaffold for RPE cells. The effects of alignment on mechanical properties, porosity, hydrophilicity, degradation of the scaffolds, and the cellular interaction of RPE cells were investigated. The results revealed that the aligned scaffold has a lower average fiber diameter, porosity, hydrophilicity, and Young’s modulus and also a higher maximum strain in failure compared with the random scaffold. However, the proliferation of RPE cells increased on the random scaffold compared to the aligned scaffold. Hence, the rest of the specialized cellular evaluations, such as immunohistochemistry, real-time PCR, and functional assessments were performed on random scaffolds. The seeded cells showed an expression of RPE signature genes and functionally secreted VEGF and PEDF. Therefore, a HAMP-based substrate was fabricated for potential use as a scaffold for RPE cell transplantation.

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Multidisciplinary Approaches in the Treatment of Retinal Degenerative Diseases: A Review

Chen,

Zhuo,

Zhu

et al. 2023

Advanced Therapeutics

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Retinal degenerative diseases (RDDs) cause irreversible blindness and affect ≈285 million individuals worldwide. The unavailability of treatment to stop the progression of RDDs or restore normal visual function necessitated development of novel therapeutic strategies. In recent years, multidisciplinary approaches have shown great potential for the treatment of RDDs, including intraocular delivery systems, retinal scaffolds for cell therapy, and visual prostheses with different stimulation strategies. This review focuses on the development prospects and shortcomings of various multidisciplinary treatment approaches for RDDs. The results of this review will hopefully inspire further innovation in the design of novel multidisciplinary approaches for RDD treatment and accelerate their commercialization.

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Development of an electrospun poly(ε-caprolactone)/collagen-based human amniotic membrane powder scaffold for culturing retinal pigment epithelial cells

Cited by 12 publications

References 65 publications

Fabrication and multiscale modeling of polycaprolactone/amniotic membrane electrospun nanofiber scaffolds for wound healing

Fabrication and multiscale modeling of polycaprolactone/amniotic membrane electrospun nanofiber scaffolds for wound healing

The Effect of Aligned and Random PCL-Human Amniotic Membrane Powder Scaffolds on Retinal Tissue Engineering

Multidisciplinary Approaches in the Treatment of Retinal Degenerative Diseases: A Review

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