Development of an ELISA for salinomycin and depletion kinetics of salinomycin residues in poultry

Kennedy, D. G.; Blanchflower, W. J.; O'Dornan, B C

doi:10.1080/02652039509374283

Cited by 24 publications

(20 citation statements)

References 6 publications

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“…Coccidiostats are not used in human medicine because of their cardiovascular effects [2]. For example, LAS has been found to cause contraction of human heart in test systems [3].…”

Section: Introductionmentioning

confidence: 99%

“…As the chemical structures and particularly the mode of action of BEA/ENNs and coccidiostats are closely related, the question raised whether these lipophilic mycotoxins could accumulate in the same animal tissues as coccidiostats [2,25]. The aims of the present study were (i) to study the suitability of the sample preparation technique of ionophoric coccidiostats for BEA and ENNs, (ii) to validate the method for the analysis of BEA and ENNs from poultry tissues (meat and liver), (iii) to add ionophoric coccidiostat MAD to the existing method for ionophoric coccidiostats, (iv) to validate the coccidiostat method for liver samples, (v) to study the simultaneous presence of coccidiostats as well as BEA and ENNs in Finnish poultry tissues.…”

Section: Introductionmentioning

confidence: 99%

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An integrated sample preparation to determine coccidiostats and emerging Fusarium‐mycotoxins in various poultry tissues with LC‐MS/MS

Jestoi¹,

Rokka

Peltonen

2007

Molecular Nutrition Food Res

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The usefulness of an existing sample preparation technique used for ionophoric coccidiostats (lasalocid, monensin, salinomycin and narasin) was applied in the analysis of emerging Fusarium-mycotoxins beauvericin (BEA) and enniatins (ENNs) in poultry tissues (liver and meat). Also, maduramicin and liver as a new sample matrix was introduced. The developed methods were validated and applied for the determination of coccidiostats and BEA/ENNs in Finnish poultry tissues in 2004-2005. The validation parameters demonstrated that the integrated sample preparation technique is applicable to the parallel determination of these contaminants in poultry tissues. Of the samples analysed (276 meat and 43 liver), only trace levels of LAS, MON, SAL, NAR and MAD were detected in 7, 3, 5, 6 and 4% of the samples, respectively. Interestingly, for the first time, traces of BEA and ENNs could also be detected in animal tissues. BEA and ENNs A, A1, B and B1 were found in 2, 0.3, 0.6, 4 and 3% of the samples, respectively. The simultaneous presence of coccidiostats and mycotoxins was detected in three turkey samples in 2004.

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“…Coccidiostats are not used in human medicine because of their cardiovascular effects [2]. For example, LAS has been found to cause contraction of human heart in test systems [3].…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

An integrated sample preparation to determine coccidiostats and emerging Fusarium‐mycotoxins in various poultry tissues with LC‐MS/MS

Jestoi¹,

Rokka

Peltonen

2007

Molecular Nutrition Food Res

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“…Coccidiostats are not used in human medicine because of their toxicity [6] principally due to their cardiovascular effects [7], e. g., lasalocid has been found to cause contraction of human heart under laboratory conditions [8]. The toxic effects of coccidiostats have been noted in many animal studies.…”

Section: Introductionmentioning

confidence: 99%

The residue levels of narasin in eggs of laying hens fed with unmedicated and medicated feed

Rokka¹,

Eerola²,

Perttilä³

et al. 2005

Mol. Nutr. Food Res.

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Laying hens were fed contaminated feed containing narasin 2.5 mg/kg for 21 days followed by a 7 day withdrawal period, hens in the control group were fed unmedicated feed. Eggs were collected during trial days 0, 3, 7, 14, 21 and after the withdrawal period of 7 days. The concentration of narasin in yolks and egg whites was analyzed by a liquid chromatography-mass spectrometry method. Narasin was found to accumulate in yolks, where the narasin concentration increased during the treatment. The concentration of narasin varied from 5.9 to 13.8 microg/kg (mean 10.6 microg/kg) in yolks after 21 day feeding periods. The concentrations of narasin ranged from < 0.9 to 1.4 microg/kg after the withdrawal period. Narasin residues were not found in egg whites of the laying hens fed contaminated feed nor in either yolks or egg whites of the laying hens fed unmedicated feed. The effect of cooking was also tested on the amount of narasin residues in eggs. Cooking for 10 min did not significantly influence the narasin residues in eggs. Traces of lasalocid were also found in the yolks. The traces of lasalocid are attributable to an accidental contamination of the feed during its manufacture.

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“…Several specific enzyme-linked immunosorbent assays (ELISA) for various ionophores have been developed 16,17 with very low detection limits, sometimes down to the low picogram level. 18 Physical-chemical confirmatory techniques, however, are usually needed to ensure that such analytical methods do not give rise to false-positive responses.…”

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confidence: 99%

Electrospray ionization and collision-induced dissociation of antibiotic polyether ionophores

Volmer¹,

Lock²

1998

Rapid Commun. Mass Spectrom.

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A series of polyether ionophores (lasalocid, monensin-A, narasin-A and salinomycin) was investigated by electrospray ionization (ESI) tandem mass spectrometry (MS/MS). A fast short-column liquid chromatographic (LC) method, using a ternary gradient sequence, was developed to separate the ionophores in less than 4 minutes. Several mobile phase parameters such as pH and solvent additives were investigated, and their impact on both chromatographic and mass spectrometric behavior was studied. The ionophores readily formed stable complexes with various metal cations present as impurities in the solvents. Therefore, a method was developed to convert the ionophores into only sodium adduct species prior to ESI analysis. This procedure maximized both sensitivity and specificity in the subsequent MS/MS step. Low energy collision-induced dissociation (CID) of the sodium adduct ions resulted in a number of structure-diagnostic product ions for all ionophores. The proposed interpretation of these fragment ions is presented in this work. Additionally, the fragmentation of a series of monovalent carboxylic alkali-metal ion complexes of lasalocid was investigated under CID conditions. A comparison of the CID spectra revealed a decreasing degree of fragmentation in the order [M + H]+ approximately [M + NH4]+ > [M + Li]+ > [M-H + 2Li]+ > [M + Na]+ >> [M + Cs]+. To demonstrate the analytical potential of the LC/MS/MS method, its application to the determination of salinomycin in catfood samples was investigated. Salinomycin was recovered from catfood by a microwave solvent extraction procedure, and subsequently analyzed using LC/MS/MS of the sodium adduct ions.

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Development of an ELISA for salinomycin and depletion kinetics of salinomycin residues in poultry

Cited by 24 publications

References 6 publications

An integrated sample preparation to determine coccidiostats and emerging Fusarium‐mycotoxins in various poultry tissues with LC‐MS/MS

An integrated sample preparation to determine coccidiostats and emerging Fusarium‐mycotoxins in various poultry tissues with LC‐MS/MS

The residue levels of narasin in eggs of laying hens fed with unmedicated and medicated feed

Electrospray ionization and collision-induced dissociation of antibiotic polyether ionophores

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