Development of an ELISA Test for Detection of Anti FMD Virus Type A87/IRN Antibody Using Irradiated Antigen

Motamedi-Sedeh, Farahnaz; Khorasani, Akbar; Mahravani, Homayoon

doi:10.21859/isv.3.3.1

Cited by 4 publications

(3 citation statements)

References 10 publications

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“…The dose of irradiation did not appear to affect the immunogenicity of the virus. Foot and mouth disease (FMD) is inactivated by irradiation within the range 40,000–44,000 Gy (Motamedi-Sedeh et al 2009 ). In guinea pigs, irradiation-attenuated FMD viruses induced neutralizing antibodies that persisted for up to 8 months, post-vaccination—a similar duration to that obtained using conventional FMD vaccine.…”

Section: Introductionmentioning

confidence: 99%

“…A gamma-irradiated inactivated cell culture derived African horse sickness viral antigen has been used in a blocking ELISA (B-ELISA) for detecting antibody to a subgroup-reactive epitope of African horse sickness virus (House et al 1996 ). Foot and mouth disease virus irradiated from 10,000 to 45,000 Gy appeared to have unaltered antigenicity in blocking ELISA tests (Motamedi-Sedeh et al 2009 ).…”

Section: Introductionmentioning

confidence: 99%

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The role of nuclear technologies in the diagnosis and control of livestock diseases—a review

Viljoen

Luckins

2012

Trop Anim Health Prod

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Nuclear and nuclear-related technologies have played an important role in animal health, particularly in relation to disease diagnosis and characterization of pathogenic organisms. This review focuses primarily on how and where nuclear technologies, both non-isotopic and isotopic methods, have made their impact in the past and where it might be expected they could have an impact in the future. The review outlines the extensive use of radiation attenuation in attempts to create vaccines for a multiplicity of pathogenic organisms and how the technology is being re-examined in the light of recent advances in irradiation techniques and cryopreservation/lyophilization that might obviate some of the problems of maintenance of viable, attenuate vaccines and their transport and use in the field. This approach could be used for a number of parasitic diseases where vaccination has been problematic and where investigations into the development of molecular vaccines have still failed to deliver satisfactory candidates for generating protective immune responses. Irradiation of antigens or serum samples also has its uses in diagnosis, especially when the samples need to be transported across international boundaries, or when handling the pathogens in question when carrying out a test presents serious health hazards to laboratory personnel. The present-day extensive use of enzyme immunoassays and molecular methods (e.g., polymerase chain reaction) for diagnosis and characterization of animal pathogens has its origins in the use of isotope-labeled antigens and antibodies. These isotopic techniques that included the use of 75Se, 32P, 125I, and 35S isotopes enabled a level of sensitivity and specificity that was hitherto unrealized, and it is prescient to remind ourselves of just how successful these technologies were, in spite of their infrequent use nowadays. Finally, the review looks at the potential for stable isotope analysis for a variety of applications—in the tracking of animal migrations, where the migrant are potential carriers of transboundary animal diseases, and where it would be useful to determine the origins of the carrier, e.g., Highly Pathogenic Avian Influenza and its dissemination by wild water fowl. Other applications could be in monitoring sequestered microbial culture (e.g., rinderpest virus) where in the case of accidental or deliberate release of infective culture it would be possible to identify the laboratory from which the isolate originated.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The role of nuclear technologies in the diagnosis and control of livestock diseases—a review

Viljoen

Luckins

2012

Trop Anim Health Prod

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“…These experimental animals and natural hosts' immune responses led to the production of virus-neutralizing antibodies and viral challenge protection [1]. For diagnostic application, VP1 protein type O was expressed in E. coli, and the purified antibodies and protein were then tested against clinical samples from various serotypes using the ELISA method [19][20][21].…”

Section: Discussionmentioning

confidence: 99%

Expression of VP1 protein of foot-and-mouth disease virus serotype O originated from Indonesia in mammalian cells as potential immunogen

Widayanti,

Suryanggono,

Pambudi

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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In 2022, Indonesia experienced foot-and-mouth disease (FMD) outbreak resulting in the death of hundreds of cattle; after more than 30 years, the country maintained FMD-free status. The FMD viral polypeptides-1 (VP1) protein contains major antigenic sites, making the protein have an important role in diagnostic applications or vaccine development. In this study, a synthetic codon-optimized vp1 gene from FMD virus serotype O was integrated into pcDNA3.1 vector and transformed to Escherichia coli TOP10F’ for plasmid propagation. The pcDNA3.1-1D-VP1 FMDV plasmid was then verified using agarose gel, showing in 659 bp size of the DNA fragment. The VP1 recombinant plasmid was transfected into HEK293T cells in DMEM medium supplemented with 10% FBS. Protein profiles were determined with SDS-PAGE showing target protein at 33KDa. Protein expression was confirmed by in-cell western assay using anti-VP1 type O polyclonal antibody and IRDye® 800CW goat anti-rabbit IgG as the secondary antibody. The result revealed a high-intensity fluorescence signal, indicating a strong interaction between expressed protein and anti-VP1 antibody. Thus, the results of this study demonstrate the potential for VP1 protein to be used as an immunogen in vaccine or diagnostic development against FMD infection. Nonetheless, some additional studies should be conducted.

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Development of Protective Immunity against Inactivated Iranian Isolate of Foot-and-Mouth Disease Virus Type O/IRN/2007 Using Gamma Ray-Irradiated Vaccine on BALB/c Mice and Guinea Pigs

Motamedi-Sedeh

Soleimanjahi²,

Jalilian³

et al. 2015

Intervirology

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Objectives: Foot-and-mouth disease virus (FMDV) causes a highly contagious disease in cloven-hoofed animals and is the most damaging disease of livestock worldwide, leading to great economic losses. The aim of this research was the inactivation of FMDV type O/IRN/1/2007 to produce a gamma ray-irradiated (GRI) vaccine in order to immunize mice and guinea pigs. Methods: In this research, the Iranian isolated FMDV type O/IRN/1/2007 was irradiated by gamma ray to prepare an inactivated whole virus antigen and formulated as a GRI vaccine with unaltered antigenic characteristics. Immune responses against this vaccine were evaluated on mice and guinea pigs. Results: The comparison of the immune responses between the GRI vaccine and conventional vaccine did not show any significant difference in neutralizing antibody titer, memory spleen T lymphocytes or IFN-γ, IL-4, IL-2 and IL-10 concentrations (p > 0.05). In contrast, there were significant differences in all of the evaluated immune factors between the two vaccinated groups of mice and negative control mice (p < 0.05). The protective dose 50 for the conventional and GRI vaccines obtained were 6.28 and 7.07, respectively, which indicated the high potency of both vaccines. Conclusion: GRI vaccine is suitable for both routine vaccination and control of FMDV in emergency outbreaks.

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Development of an ELISA Test for Detection of Anti FMD Virus Type A87/IRN Antibody Using Irradiated Antigen

Cited by 4 publications

References 10 publications

The role of nuclear technologies in the diagnosis and control of livestock diseases—a review

The role of nuclear technologies in the diagnosis and control of livestock diseases—a review

Expression of VP1 protein of foot-and-mouth disease virus serotype O originated from Indonesia in mammalian cells as potential immunogen

Development of Protective Immunity against Inactivated Iranian Isolate of Foot-and-Mouth Disease Virus Type O/IRN/2007 Using Gamma Ray-Irradiated Vaccine on BALB/c Mice and Guinea Pigs

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