Development of an engineering autologous palatal mucosa‐like tissue for potential clinical applications

Abstract

The goal of this study was to optimize key processes in recreating functional and viable palatal mucosa-like tissue that would be easy to handle and would promote wound healing. Normal human gingival fibroblasts and epithelial cells and a clinically useful biomaterial, CollaTape, were used. Structural and ultrastructural analyses showed that the gingival fibroblasts and epithelial cells adhered to the biomaterial and proliferated. Following a 6-day culture, using 10(5) fibroblasts and 10(6) epithelial cells, a well-organized palatal mucosa-like tissue was engineered. The engineered epithelium displayed various layers, including a stratum corneum, and contained cytokeratin 16-positive cells located in the supra-basal layer. This palatal mucosa-like engineered tissue was designed to meet a variety of surgical needs. The biodegradable collagen membrane (CollaTape) contributed to the flexibility of the engineered tissue. This engineered innovative tissue may contribute to the reconstruction of oral soft-tissue defects secondary to trauma, congenital defects, and acquired diseases.

Chitosan-Coated Collagen Membranes Promote Chondrocyte Adhesion, Growth, and Interleukin-6 Secretion

“…The CollaTape is a biocompatible three-dimentional porous soft collagen sponge easy to handle with good mechanical properties. It is already approved for human use basically in dental surgery, but also as a scaffold for in vitro [27] and in vivo tissue engineering [28]. Because of the coherent sponge structure and composition, its use in this study will be of great interest in engineering a chitosan-collagen composite scaffold.…”

Cryopreserved lip mucosa tissue derived keratinocytes can fabricate tissue engineered palatal mucosa equivalent

“…It has been reported that wound contraction and scar formation on the palate after palatoplasty caused the maxillary growth retardation 3. Presently, extensive literatures suggested that by using tissue engineered palatal mucosa substitutes to cover the denuded bone wounds could reduce the extent of wound contraction and scar formation 4–6…”

In Vitro Evaluation of Acellular Dermal Matrix as a Three‐Dimensional Scaffold for Gingival Fibroblasts Seeding

“…Cell culture into different matrices, including allogeneic skin substitutes, has been studied and has shown promising results in the regeneration of soft tissues 5‐14 . The acellular dermal matrix (ADM) † in particular is probably the most used and studied of the allogeneic subcutaneous substitutes.…”