Development of an enzyme-linked immunosorbent assay (ELISA) for natamycin residues in foods based on a specific monoclonal antibody

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A specific monoclonal antibody (mAb) against trimethoprim (TMP) was produced and a mAb-based indirect competitive enzymelinked immunosorbent assay (ic-ELISA) was established to detect TMP residues in milk, honey, and fish samples. The working range of ic-ELISA and IC 50 was 1.83-9.36 and 4.14 µg L −1 . The average recoveries of TMP from TMP-spiked milk, honey, and fish sample were 103.5-118.0%, 100.3-107.1%, and 91.5-108.1%, respectively. The results revealed that our developed mAb-based ic-ELISA can be effectively applied to detect TMP residues in these food products. ARTICLE HISTORY

“…BSA or OVA) (Chen et al, 2015). Briefly, 13 mg of TMP previously dissolved in 3 mL of acetonitrile was mixed with 50 µL of 25% GA solution for reacting for 5 min.…”

Section: Synthesis Of Immunogens and Coating Antigensmentioning

confidence: 99%

Establishment of a monoclonal antibody-based indirect enzyme-linked immunosorbent assay for the detection of trimethoprim residues in milk, honey, and fish samples

Liu

Song

Kuang

et al. 2016

Self Cite

“…The linear range of the assay was from 3.53 to 41.98 ng mL −1 (IC 20 -IC 80 ). IC 50 value is the concentration of small molecular which generates a 50% inhibition of antibody binding to the antigen (Chen et al, 2015;Scharnweber et al, 2001). It was considered a sensitivity measure of the mAb.…”

Section: Sensitivity and Calibration Curvementioning

confidence: 99%

Development of an indirect competitive ELISA for the detection of acenaphthene and pyrene

Qiao

Meng

et al. 2017

Development of an indirect competitive ELISA for the detection of acenaphthene and pyrene, Food and Agricultural Immunology, 28:5, 789-800, ABSTRACTPolycyclic aromatic hydrocarbons (PAHs) have mutagenic and carcinogenic properties. Acenaphthene and pyrene were members of 16 PAHs which were listed as the priority pollutants in water environment by US Environmental Protection Agency. The reported instrumental methods and immunoassays did not meet the need for simple and sensitive detection of acenaphthene and pyrene. In this study, a monoclonal antiobody having high affinities with acenaphthene and pyrene was produced and an indirect competitive enzyme-linked immunosorbent assay (ELISA) was developed for sensitive detection of acenaphthene and pyrene in water sample. The linear range of the assay was between 3.53 and 41.98 ng mL −1 . The sensitivity was 12.17 ng mL −1 which was more sensitive than those of reported ELISAs. The average recovery of acenaphthene and pyrene from three kinds of water samples was 99.08% and 98.45, respectively. The developed ELISA could be used for sensitive detection of acenaphthene and pyrene in water samples. ARTICLE HISTORY

“…The hybridomas were cultured in HAT (H refers to hypoxanthine, A refers to aminopterin and T refers to thymidine) medium at 37°C and 5% CO 2 . Half of the culture medium was replaced with fresh HAT medium on day 3 and completely replaced with HT (H refers to hypoxanthine and T refers to thymidine) medium on day 5 (Chen, Kong, et al, 2015;Suryoprabowo, Liu, Peng, Kuang, & Xu, 2014). The supernatants were analyzed by indirect ELISA on day 7.…”

Section: Cell Strain Preparationmentioning

confidence: 99%

Sandwich ELISA and immunochromatographic strip of Kunitz trypsin inhibitor using sensitive monoclonal antibodies

Song

Liu

et al. 2016

Self Cite

Kunitz trypsin inhibitor (KTI) is a predominant anti-nutritional factor in soybean. The objective of this study was to develop a sandwich enzyme-linked immunosorbent assay and immunochromato graphic strip (ICS) method for the semi-quantification of KTI in soybean. The limit of detection was 0.1481 ng/ml, and the linear dynamic range was 0.2-20 ng/ml. KTI recovery was 98.40-110.66% from KTI-spiked milk samples, with coefficients of variation of 4.41-10.62%. Based on ICS results, KTI had cutoff values of 5 ng/ ml and 2.5 ng/ml in 0.05% Tween-added phosphate-buffered saline and milk, respectively. ICS has potential applications for the fast detection of KTI.