Development of an enzyme-linked immunosorbent assay for the determination of florfenicol and florfenicol amine in eggs

Abstract

A monoclonal antibody against florfenicol amine (FFA) was produced, and a competitive enzyme-linked immunosorbent assay (ELISA) was developed for detecting florfenicol (FF) and FFA in eggs. This proposed method exhibited the 50% inhibiting concentrations (IC 50 ) of 0.120 ng/mL for FF and 0.118 ng/mL for FFA under the optimization of experimental parameters. At the fortified levels of 0.4, 0.8, 2.4, and 30.0 μg/kg, the inter-assay recoveries of the target compounds ranged from 78.3 to 103.2%, and the intra-assay recoveries ranged from 81.5 to 104.1%, respectively. The coefficients of variation were all below 12%. Results for the determination of spiked samples by this ELISA showed a good relationship with the high-performance liquid chromatography (HPLC) technique. This newly developed method confirmed to be rapid, user-friendly, and sensitivity for simultaneous detecting FF and FFA in eggs.

Rapid and sensitive detection of chlordimeform in cucumber and tomato samples using an immunochromatographic assay

“…MAb production consists of three steps: mouse immunization, cell fusion, and ascites fluid purification. 26,27 First, five female mice (6-weeks old) were immunized five times (a dose of 100 μg at the first immunization and 50 μg at booster immunization) by subcutaneous injection using CDM-S 1 -BSA, with an interval of three weeks. 28 The positive mouse with high affinity and sensitivity was screened by ic-ELISA, and immunized by intraperitoneal injection (25 μg) after the last booster immunization.…”

Preparation of a broad-specific monoclonal antibody and development of an immunochromatographic assay for monitoring of anthranilic diamides in vegetables and fruits

“…24,25 Immunoassays are alternative methods for the screening of various hazardous substances in the environment and foods. [26][27][28][29] In the case of anthranilic diamide insecticides, Zhang et al prepared a monoclonal antibody (mAb) and developed an indirect competitive ELISA (ic-ELISA) for the rapid detection of CYA. 30 The ic-ELISA provided a detection range of 0.43-6.15 μg L −1 and good recovery in pakchoi and tap water samples.…”