Development of an equine carpal groove model to study early changes in osteoarthritis - a pilot study

Moller, Nikae te

doi:10.1016/j.joca.2018.02.288

Cited by 6 publications

(8 citation statements)

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“…In a 10-week pilot study in Shetland ponies, we showed later that the model behaved similarly in middle carpal and MCP joints. 8 The cited studies focused on structural changes of cartilage and subchondral bone, biochemical analysis of cartilage, and, to some extent, synovial fluid (SF) biomarkers. [4][5][6][7] Data on biomechanical changes of the cartilage is still limited, despite the growing awareness of the crucial role of biomechanics in our current incapacity to regenerate functional articular cartilage.…”

Section: Introductionmentioning

confidence: 99%

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Structural, compositional, and functional effects of blunt and sharp cartilage damage on the joint: A 9‐month equine groove model study

Moller

Mohammadi

Plomp

et al. 2021

Journal Orthopaedic Research

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This study aimed to quantify the long-term progression of blunt and sharp cartilage defects and their effect on joint homeostasis and function of the equine carpus. In nine adult Shetland ponies, the cartilage in the radiocarpal and middle carpal joint of one front limb was grooved (blunt or sharp randomized). The ponies were subjected to an 8-week exercise protocol and euthanized at 39 weeks. Structural and compositional alterations in joint tissues were evaluated in vivo using serial radiographs, synovial biopsies, and synovial fluid samples. Joint function was monitored by quantitative gait analysis. Macroscopic, microscopic, and biomechanical evaluation of the cartilage and assessment of subchondral bone parameters were performed ex vivo. Grooved cartilage showed higher OARSI microscopy scores than the contralateral sham-operated controls (p < 0.0001). Blunt-grooved cartilage scored higher than sharp-grooved cartilage (p = 0.007) and fixed charge density around these grooves was lower (p = 0.006). Equilibrium and instantaneous moduli trended lower in grooved cartilage than their controls (significant for radiocarpal joints). Changes in other tissues included a threefold to sevenfold change in interleukin-6 expression in synovium from grooved joints at week 23 (p = 0.042) and an increased CPII/C2C ratio in synovial fluid extracted from blunt-grooved joints at week 35 (p = 0.010).Gait analysis outcome revealed mild, gradually increasing lameness. In conclusion, blunt and, to a lesser extent, sharp grooves in combination with a period of moderate exercise, lead to mild degeneration in equine carpal cartilage over a 9-month period, but the effect on overall joint health remains limited.

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Section: Introductionmentioning

confidence: 99%

“…In a 10‐week pilot study in Shetland ponies, we showed later that the model behaved similarly in middle carpal and MCP joints. 8 …”

Section: Introductionmentioning

confidence: 99%

Structural, compositional, and functional effects of blunt and sharp cartilage damage on the joint: A 9‐month equine groove model study

Moller

Mohammadi

Plomp

et al. 2021

Journal Orthopaedic Research

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“…Nine female Shetland ponies (aged 6.8 ± 2.6 years; bodyweight was 203 ± 15.3 kg) were included. As no prior contrast agent (ioxaglate) partition data for whole equine joints were available, the number of ponies was based on a power analysis (power 0.90 and P < 0.05) pertaining to the macroscopic and microscopic scores (OARSI) in previous studies 8,9,24‐26 . Retrospectively, based on the ioxaglate partitions as determined in this study, the statistical power was found to range from 0.83 to 0.94, depending on the location and the groove type.…”

Section: Methodsmentioning

confidence: 90%

Dual‐contrast micro‐CT enables cartilage lesion detection and tissue condition evaluation ex vivo

Honkanen

Mohammadi

Moller

et al. 2022

Equine Veterinary Journal

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Background: Post-traumatic osteoarthritis is a frequent joint disease in the horse.Currently, equine medicine lacks effective methods to diagnose the severity of chondral defects after an injury.Objectives: To investigate the capability of dual-contrast-enhanced computed tomography (dual-CECT) for detection of chondral lesions and evaluation of the severity of articular cartilage degeneration in the equine carpus ex vivo.

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“…Nine healthy adult female Shetland ponies (6.8 ± 2.6 years; range 4–13 years) were used for this study. The number of ponies was chosen after a power analysis (power 0.9 and p < 0.05) based on a pilot study 52 and previous groove model studies. 7 , 34 , 36 Briefly, blunt and sharp grooves were made by arthrotomy, performed by a European board‐certified equine surgeon (HB) in one randomly assigned front limb per animal.…”

Section: Methodsmentioning

confidence: 99%

Site- and Zone-Dependent Changes in Proteoglycan Content and Biomechanical Properties of Bluntly and Sharply Grooved Equine Articular Cartilage

et al. 2022

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In this study, we mapped and quantified changes of proteoglycan (PG) content and biomechanical properties in articular cartilage in which either blunt or sharp grooves had been made, both close to the groove and more remote of it, and at the opposing joint surface (kissing site) in equine carpal joints. In nine adult Shetland ponies, standardized blunt and sharp grooves were surgically made in the radiocarpal and middle carpal joints of a randomly chosen front limb. The contralateral control limb was sham-operated. At 39 weeks after surgery, ponies were euthanized. In 10 regions of interest (ROIs) (six remote from the grooves and four directly around the grooves), PG content as a function of tissue-depth and distance-to-groove was estimated using digital densitometry. Biomechanical properties of the cartilage were evaluated in the six ROIs remote from the grooves. Compared to control joints, whole tissue depth PG loss was found in sites adjacent to sharp and, to a larger extent, blunt grooves. Also, superficial PG loss of the surgically untouched kissing cartilage layers was observed. Significant PG loss was observed up to 300 µm (sharp) and at 500 µm (blunt) from the groove into the surrounding tissue. Equilibrium modulus was lower in grooved cartilage than in controls. Grooves, in particular blunt grooves, gave rise to severe PG loss close to the grooved sites and to mild degeneration more remote from the grooves in both sharply and bluntly grooved cartilage and at the kissing sites, resulting in loss of mechanical strength over the 9-month period.

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Development of an equine carpal groove model to study early changes in osteoarthritis - a pilot study

Cited by 6 publications

References 0 publications

Structural, compositional, and functional effects of blunt and sharp cartilage damage on the joint: A 9‐month equine groove model study

Structural, compositional, and functional effects of blunt and sharp cartilage damage on the joint: A 9‐month equine groove model study

Dual‐contrast micro‐CT enables cartilage lesion detection and tissue condition evaluation ex vivo

Site- and Zone-Dependent Changes in Proteoglycan Content and Biomechanical Properties of Bluntly and Sharply Grooved Equine Articular Cartilage

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