Development of an Immunochromatographic Assay Kit Using Fluorescent Silica Nanoparticles for Rapid Diagnosis of Acanthamoeba Keratitis

Toriyama, Koji; Suzuki, Takashi; Eguchi, Hiroshi; Hoshi, Saichi; Inoue, Yoshitsugu; Aizawa, Hideki; Miyoshi, K.; Ohkubo, M.; Hiwatashi, Eiji; Tachibana, Hiroshi; Ohashi, Yuichi

doi:10.1128/jcm.02595-14

Cited by 18 publications

(15 citation statements)

References 32 publications

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“…However, their application efficacy for OPPP monitoring in premise plumbing is still subject to the concentration level of target cells and availability of high quality antibodies. It has been reported that selected antibodies might not be able to cover the whole range of serogroups or species (Keserue et al, 2012) and validation of specificity and sensitivity against environmental background is often lacking (Toriyama et al, 2015; Visvesvara et al, 1987). …”

Section: Detection Methodsmentioning

confidence: 99%

Methodological approaches for monitoring opportunistic pathogens in premise plumbing: A review

et al. 2017

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Opportunistic pathogens inhabiting premise (i.e., building) plumbing (OPPPs, e.g., L. pneumophila, M. avium complex, P. aeruginosa, Acanthamoeba, and N. fowleri) are a significant and growing source of disease. Because OPPPs establish and grow as part of the native drinking water microbiota, they do not correspond to fecal indicators, presenting a significant challenge to common and effective monitoring strategies. Further, different OPPPs present distinct requirements for sampling, preservation, and analysis, creating a significant impediment to their parallel detection. The aim of this critical review is to synthesize the state of the science of monitoring OPPPs and to identify a path forward for their simultaneous detection and quantification in a manner commensurate with the need for reliable data to inform risk assessment and mitigation. Water and biofilm sampling procedures, as well as factors influencing sample representativeness and detection sensitivity, are critically evaluated with respect to the five representative bacterial and amoebal OPPPs noted above. Available culturing and molecular approaches are discussed in terms of their advantages, limitations, and applicability. Knowledge gaps and research needs are identified.

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Section: Detection Methodsmentioning

confidence: 99%

Methodological approaches for monitoring opportunistic pathogens in premise plumbing: A review

et al. 2017

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“…An aggregation-based assay was demonstrated by Jayawardana and co-workers (2015) for the detection of the model bacteria Mycobacterium smegmatis. Japanese company Furukawa Electric has used commercialised a lateral flow assay incorporating fluorescent silica NPs, called Quartz Dots (290 nm) for the detection of different infectious microorganisms (Toriyama et al 2015).…”

Section: Immunoassays and Biosensorsmentioning

confidence: 99%

Dye-doped silica nanoparticles: synthesis, surface chemistry and bioapplications

et al. 2020

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Background: Fluorescent silica nanoparticles have been extensively utilised in a broad range of biological applications and are facilitated by their predictable, wellunderstood, flexible chemistry and apparent biocompatibility. The ability to couple various siloxane precursors with fluorescent dyes and to be subsequently incorporated into silica nanoparticles has made it possible to engineer these fluorophores-doped nanomaterials to specific optical requirements in biological experimentation. Consequently, this class of nanomaterial has been used in applications across immunodiagnostics, drug delivery and human-trial bioimaging in cancer research. Main body: This review summarises the state-of-the-art of the use of dye-doped silica nanoparticles in bioapplications and firstly accounts for the common nanoparticle synthesis methods, surface modification approaches and different bioconjugation strategies employed to generate biomolecule-coated nanoparticles. The use of dye-doped silica nanoparticles in immunoassays/biosensing, bioimaging and drug delivery is then provided and possible future directions in the field are highlighted. Other non-cancerrelated applications involving silica nanoparticles are also briefly discussed. Importantly, the impact of how the protein corona has changed our understanding of NP interactions with biological systems is described, as well as demonstrations of its capacity to be favourably manipulated. Conclusions: Dye-doped silica nanoparticles have found success in the immunodiagnostics domain and have also shown promise as bioimaging agents in human clinical trials. Their use in cancer delivery has been restricted to murine models, as has been the case for the vast majority of nanomaterials intended for cancer therapy. This is hampered by the need for more human-like disease models and the lack of standardisation towards assessing nanoparticle toxicity. However, developments in the manipulation of the protein corona have improved the understanding of fundamental bio-nano interactions, and will undoubtedly assist in the translation of silica nanoparticles for disease treatment to the clinic.

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“…5(e)). 78 Silica nanoparticles doped with cyanine-5 (Cy5), the most commonly used fluorescent dye in biological assays, has been reported for use in the development of fluorescence-based lateral flow immunoassay (LFIA) for influenza A antigen detection. Carboxyl-modified silica nanoparticles (50 nm), prepared by Stober method, were linked to the amino group of monoclonal antibody specific to influenza A nucleoprotein through carbodiimide chemistry.…”

Section: •2•2 Silica Nanoparticle As a Tracing Tag To Label Signalmentioning

confidence: 99%

Nanoparticle-based Point of Care Immunoassays for in vitro Biomedical Diagnostics

2018

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In resource-limited settings, the availability of medical practitioners and early diagnostic facilities are inadequate relative to the population size and disease burden. To address cost and delayed time issues in diagnostics, strip-based immunoassays, e.g. dipstick, lateral flow assay (LFA) and microfluidic paper-based analytical devices (microPADs), have emerged as promising alternatives to conventional diagnostic approaches. These assays rely on chromogenic agents to detect disease biomarkers. However, limited specificity and sensitivity have motivated scientists to improve the efficiency of these assays by conjugating chromogenic agents with nanoparticles for enhanced qualitative and quantitative output. Various nanomaterials, which include metallic, magnetic and luminescent nanoparticles, are being used in the fabrication of biosensors to detect and quantify biomolecules and disease biomarkers. This review discusses some of the principles and applications of such nanoparticle-based point of care biosensors in biomedical diagnosis.

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Development of an Immunochromatographic Assay Kit Using Fluorescent Silica Nanoparticles for Rapid Diagnosis of Acanthamoeba Keratitis

Cited by 18 publications

References 32 publications

Methodological approaches for monitoring opportunistic pathogens in premise plumbing: A review

Methodological approaches for monitoring opportunistic pathogens in premise plumbing: A review

Dye-doped silica nanoparticles: synthesis, surface chemistry and bioapplications

Nanoparticle-based Point of Care Immunoassays for in vitro Biomedical Diagnostics

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