Development of an immunochromatographic assay for the detection of alternariol in cereal and fruit juice samples

Kong, Dezhao; Xie, Zhengjun; Liu, Liqiang; Song, Shanshan; Zheng, Qinfen; Kuang, Hua

doi:10.1080/09540105.2017.1326469

Cited by 13 publications

(9 citation statements)

References 28 publications

(28 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The first antibodies and immunoassays for AOH were reported in 2011 [10,11]. Since then, a few immunoassays have been described using either polyclonal or monoclonal antibodies specific to AOH [12,13], and only one study has been published using an antibody specific to AME [14]. Additionally, Wang et al have reported a generic antibody for both mycotoxins [15].…”

Section: Introductionmentioning

confidence: 99%

Assessment of the Optimum Linker Tethering Site of Alternariol Haptens for Antibody Generation and Immunoassay Development

Addante-Moya

Abad‐Somovilla

Abad‐Fuentes

et al. 2021

Toxins

View full text Add to dashboard Cite

Immunochemical methods for mycotoxin analysis require antigens with well-defined structures and antibodies with outstanding binding properties. Immunoreagents for the mycotoxins alternariol and/or alternariol monomethyl ether have typically been obtained with chemically uncharacterized haptens, and antigen conjugates have most likely been prepared with mixtures of functionalized molecules. For the first time, total synthesis was performed, in the present study, to obtain two haptens with opposite linker attachment locations. The functionalized synthetic haptens were purified and deeply characterized by different spectrometric methods, allowing the preparation of bioconjugates with unequivocal structures. Direct and indirect competitive enzyme-linked immunosorbent assays, using homologous and heterologous conjugates, were employed to extensively evaluate the generated immunoreagents. Antibodies with high affinity were raised from conjugates of both haptens, and a structure-activity relationship between the synthetic haptens and the specificity of the generated antibodies could be established. These results pave the way for the development of novel highly sensitive immunoassays selective of one or two of these Alternaria mycotoxins.

show abstract

Section: Introductionmentioning

confidence: 99%

Assessment of the Optimum Linker Tethering Site of Alternariol Haptens for Antibody Generation and Immunoassay Development

Addante-Moya

Abad‐Somovilla

Abad‐Fuentes

et al. 2021

Toxins

View full text Add to dashboard Cite

show abstract

“…This is because a pH that is too high or too low will affect the physical and chemical properties of antigens and antibodies. 16 The results of optimization are revealed in Figure 4 A. From the test results, the pH value was in the range of 4.0–8.6, the antibody titer was basically unchanged, and the highest IC 50 value was at pH 7.4, so pH 7.4 was selected.…”

Section: Resultsmentioning

confidence: 99%

“…Parameters such as the quantity of gold-nanoparticle-labeled monoclonal antibody and the coating antigen, as well as the suspension buffer, have a certain influence on the analytical performance and sensitivity of a lateral-flow ICA strip. 16 Each condition was analyzed with a negative sample (0 ng/mL DAS) and a positive sample (25 ng/mL DAS).…”

Section: Resultsmentioning

confidence: 99%

Development of an ic-ELISA and Immunochromatographic Strip Assay for the Detection of Diacetoxyscirpenol in Rice

Jin

Cui

et al. 2020

ACS Omega

Self Cite

View full text Add to dashboard Cite

Diacetoxyscirpenol (DAS) is a highly toxic type A trichothecene, a natural contaminant in food and animal feed, which is a serious hazard to human and animal health. An anti-DAS mAb, 3H10, with high sensitivity and specificity, was prepared, and an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and a lateral-flow immunochromatographic strip (ICA strip) were developed for rapid testing of DAS in rice samples. The 50% inhibitory concentration and limit of detection of ic-ELISA were 5.97 and 0.78 ng/mL, respectively. The recovery of rice samples ranged from 99.4 to 110.7%, demonstrating that the analytical method can be used to detect rice samples. The cutoff value of the lateral-flow ICA strip to DAS was 500 ng/g. The developed immunoassay method can provide an effective method of initially detecting and screening DAS in food and feed samples.

show abstract

“…In the last decade, liquid chromatography–tandem mass spectrometry (LC-MS/MS) ( Krausová et al, 2021 ), immunoassay ( Ackermann et al, 2011 ; Burkin and Kononenko, 2011 ; Kong et al, 2017 ; Man et al, 2017 ; Singh et al, 2018 ; Wang et al, 2018 ; Cai et al, 2021 ; Liang et al, 2021 ) and other methods with molecularly imprinted polymer or aptamers as core reagents were used to detect Alternaria mycotoxins in food and biology samples ( Quílez-Alburquerque et al, 2021 ; Wang et al, 2022 ). Given the advantages of simple operation, less time consumption, and low cost, immunological methods were considered to be the most suitable method for the on-site detection of Alternaria mycotoxins in food samples ( Ackermann et al, 2011 ), and an antibody was supposed to be the most important reagent.…”

Section: Introductionmentioning

confidence: 99%

Recombinant Antibody-Based and Computer-Aided Comprehensive Analysis of Antibody’s Equivalent Recognition Mechanism of Alternariol and Alternariol Monomethyl Ether

et al. 2022

View full text Add to dashboard Cite

Alternariol (AOH) and alternariol monomethyl ether (AME) are two main Alternaria mycotoxins that endanger human health. In this study, a single-chain antibody fragment (scFv) capable of equivalently and specifically recognizing AOH and AME was first expressed, and its equivalent recognition mechanism was discussed. According to molecular docking and dynamic simulation, the C9 site, which was always exposed outside the binding cavity, made the structural differences between AOH and AME negligible. Due to the high similarity of structures, AOH and AME interacted with almost the same amino acids on the scFv; thus, the same interaction mode and interaction force were produced. This was considered to be the most critical reason for the equivalent recognition. Thus, the exposure of common structures was considered a potential strategy to obtain the equivalent recognition antibodies, and C9 was considered the key site in the process of hapten modification. These results laid a theoretical foundation for further research on antibodies against Alternaria mycotoxins. It could promote the rapid detection of AOH and AME in food and provide a new idea for targeted preparation of antibodies that could recognize multiple hazards with similar structures.

show abstract

Development of an immunochromatographic assay for the detection of alternariol in cereal and fruit juice samples

Cited by 13 publications

References 28 publications

Assessment of the Optimum Linker Tethering Site of Alternariol Haptens for Antibody Generation and Immunoassay Development

Assessment of the Optimum Linker Tethering Site of Alternariol Haptens for Antibody Generation and Immunoassay Development

Development of an ic-ELISA and Immunochromatographic Strip Assay for the Detection of Diacetoxyscirpenol in Rice

Recombinant Antibody-Based and Computer-Aided Comprehensive Analysis of Antibody’s Equivalent Recognition Mechanism of Alternariol and Alternariol Monomethyl Ether

Contact Info

Product

Resources

About