Development of an immunochromatographic assay for the rapid detection of bromoxynil in water

Zhu, Jiang; Chen, Wenchao; Lu, Yitong; Cheng, Guohua

doi:10.1016/j.envpol.2007.12.020

Cited by 43 publications

(14 citation statements)

References 29 publications

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“…Figure 5 shows the immunochromatographic detection of parathion-methyl. These results indicate that the sensitivity of the newly developed ICA is lower than that of the ELISA, but this phenomenon was usually observed during the strip test [36].…”

Section: Resultsmentioning

confidence: 80%

“…Several investigators have reported that the average particle diameter of 40 nm colloidal gold was the optimal particle size for most diagnostic applications because of its trade-off between the required visibility and steric hindrance [34]–[36]. Therefore, in this study, nanometer colloidal gold with an average particle diameter of 40 nm was prepared according to the method described by Frens [37].…”

Section: Methodsmentioning

confidence: 99%

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Development of an Enzyme Linked Immunosorbent Assay and an Immunochromatographic Assay for Detection of Organophosphorus Pesticides in Different Agricultural Products

et al. 2012

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ObjectiveOrganophosphorus (OP) pesticides are considered hazardous substances because of their high toxicity to nontarget species and their persistence in the environment and agricultural products. Therefore, it is important to develop a rapid, sensitive, and economical method for detecting OP pesticides and their residues in food and the environment.MethodsA broad, selective monoclonal antibody (MAb) for organophosphorus pesticides was produced. Based on the MAb, an enzyme linked immunosorbent assay (ELISA) and an immunochromatography assay (ICA) for detecting OP pesticides in different agricultural products were developed using a binding inhibition format on microtiter plates and a membrane strip, respectively.ResultsUnder the optimized conditions, the IC50 values of the ELISA ranged from 3.7 to 162.2 ng mL–1 for the 8 OP pesticides. The matrix interferences of Apple, Chinese cabbage, and greengrocery were removed by 40-fold dilution, the recoveries from spiked samples ranged from 79.1% to 118.1%. The IC50 values of ICA for the 8 OP pesticides ranged from 11.8 to 470.4 ng mL−1. The matrix interference was removed from the Chinese cabbage and Apple samples with 5-fold dilution, and the interference was removed from the greengrocery samples with 20-fold dilution. The recoveries from the spiked samples ranged between 70.6 and 131.9%. The established ELISA and ICA were specific selectivity for the 8 OP pesticides.ConclusionsThe established ELISA is a sensitive screening method for the detection of OP pesticides, but the ELISA detection method depends on a laboratory platform and requires a relative long assay time and several steps operation. The established ICA is very useful as a screening method for the quantitative, semi-quantitative or qualitative detection of OP pesticides in agricultural products, and it has advantages over ELISA methods with regard to factors such as the testing procedure, testing time, and matrix interferences, among others.

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Section: Resultsmentioning

confidence: 80%

Section: Methodsmentioning

confidence: 99%

Development of an Enzyme Linked Immunosorbent Assay and an Immunochromatographic Assay for Detection of Organophosphorus Pesticides in Different Agricultural Products

et al. 2012

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“…Several research studies reported that the average particle diameter of 40 nm colloidal gold was the optimal particle size for most diagnostic applications due to its trade-off between required visibility and steric hindrance (Chandler et al, 2000;Zhou et al, 2004;Zhu et al, 2008). Thus, in this study, nanometer colloidal gold with an average particle diameter of 40 nm was prepared according to the method described by Frens (1973).…”

Section: Preparation Of Colloidal Goldmentioning

confidence: 97%

Development of an immunochromatographic assay for the rapid detection of chlorpyrifos-methyl in water samples

Hua

Qian

Yang

et al. 2010

Biosensors and Bioelectronics

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“…Human pregnancy and urine glucose test strips were the first tests to be commercialized (Bazin et al 2010). Currently, ICA has gai ned considerable interest in various areas, including agriculture, environmental science, medicine, veterinary medicine and the food industry (Molinelli et al 2008, Zhu et al 2008, Zhou et al 2009). However, few colloidal gold-based ICA methods have been reported for the detection of aquatic animal pathogens.…”

Section: Introductionmentioning

confidence: 99%

Rapid immunochromatographic test strip to detect swimming crab Portunus trituberculatus reovirus

Zhang

Liu³

et al. 2015

Dis. Aquat. Org.

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Swimming crab reovirus (SCRV) is the causative agent of a serious disease with high mortality in cultured Portunus trituberculatus. A rapid immunochromatographic assay (ICA) was developed in a competitive assay format and optimized for the detection of SCRV. The gold probebased ICA test comprised SCRV antigen and goat anti-chicken egg yolk antibody (IgY) sprayed onto a nitrocellulose membrane as the test line and control line, respectively. IgY-gold complexes were deposited onto the conjugate pad as detector reagents. The method showed high specificity with no cross-reactivity with other related aquatic pathogens. The detection limit of the ICA strip was 50 µg ml −1. To evaluate the performance of the ICA test, the strip and an enzyme-linked immunosorbent assay (ELISA) were applied to the same samples (n = 90 crabs). The strip successfully detected SCRV in all of the artificially infected samples. Furthermore, the ICA strip and ELISA tests had high consistency (98.28%). The strip assay requires no instruments and has a detection time of less than 10 min. It is portable and easy to perform in the field. These results indicated that the developed strip could be a promising on-site tool for screening pooled crabs to confirm SCRV infection or disease outbreaks.

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Development of an immunochromatographic assay for the rapid detection of bromoxynil in water

Cited by 43 publications

References 29 publications

Development of an Enzyme Linked Immunosorbent Assay and an Immunochromatographic Assay for Detection of Organophosphorus Pesticides in Different Agricultural Products

Development of an Enzyme Linked Immunosorbent Assay and an Immunochromatographic Assay for Detection of Organophosphorus Pesticides in Different Agricultural Products

Development of an immunochromatographic assay for the rapid detection of chlorpyrifos-methyl in water samples

Rapid immunochromatographic test strip to detect swimming crab Portunus trituberculatus reovirus

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