Development of an immunochromatographic lateral flow test strip for detection of β-adrenergic agonist Clenbuterol residues

Zhang, G.P.; Wang, X.N.; Yang, Jifei; Yu, Yang; Xing, Guangxu; Li, Q.M.; Zhao, Dong; Chai, Shujun; Guo, J.Q.

doi:10.1016/j.jim.2006.02.017

Cited by 140 publications

(89 citation statements)

References 18 publications

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“…As material of the label nowadays colloidal gold is used most often [1, 5, 7, 10, 15, 18-20, 33, 34, 51, 53, 54, 57, for PAPP-A/proMBP [52] PPV Positive predictive value of indicated metabolic disorder, PAPP-A pregnancy-associated plasma protein A, proMBP pro form of eosinophil major basic protein 61,66,67,71,[75][76][77]. A runner-up is the use of coloured latex particles [21,76,78].…”

Section: Materials and Size Of The Labelmentioning

confidence: 99%

Lateral flow (immuno)assay: its strengths, weaknesses, opportunities and threats. A literature survey

2008

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Lateral flow (immuno)assays are currently used for qualitative, semiquantitative and to some extent quantitative monitoring in resource-poor or non-laboratory environments. Applications include tests on pathogens, drugs, hormones and metabolites in biomedical, phytosanitary, veterinary, feed/food and environmental settings. We describe principles of current formats, applications, limitations and perspectives for quantitative monitoring. We illustrate the potentials and limitations of analysis with lateral flow (immuno)assays using a literature survey and a SWOT analysis (acronym for "strengths, weaknesses, opportunities, threats"). Articles referred to in this survey were searched for on MEDLINE, Scopus and in references of reviewed papers. Search terms included "immunochromatography", "sol particle immunoassay", "lateral flow immunoassay" and "dipstick assay".

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Section: Materials and Size Of The Labelmentioning

confidence: 99%

Lateral flow (immuno)assay: its strengths, weaknesses, opportunities and threats. A literature survey

2008

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“…[2][3][4][5][6][7][8][9][10][11][12][13][14][15] Some of these tests analyze environmental conditions, 16,17 or detect illness (often in the developing world). [18][19][20] We have recently patterned paper into hydrophilic regions demarcated by hydrophobic walls using photolithography and conventional photoresists (SU-8; and poly(methyl methacrylate), or PMMA).…”

Section: Introductionmentioning

confidence: 99%

Low-Cost Printing of Poly(dimethylsiloxane) Barriers To Define Microchannels in Paper

2008

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This paper describes the use of a modified x,y-plotter to generate hydrophilic channels by printing a solution of hydrophobic polymer (poly(dimethyl siloxane; PDMS) dissolved in hexanes onto filter paper. The PDMS penetrates the depth of the paper, and forms a hydrophobic wall that aqueous solutions cannot cross. The minimum size of printed features is approximately 1 mm; this resolution is adequate for the rapid prototyping of hand-held, visually read, diagnostic assays (and other microfluidic systems) based on paper. After curing the printed PDMS, the paper-based devices can be bent or folded to generate three-dimensional (3D) systems of channels. Capillary action pulls aqueous samples into the paper channels. Colorimetric assays for the presence of glucose and protein are demonstrated in the printed devices; spots of Bromothymol Blue distinguished samples with slightly basic pH (6.5) from samples with slightly acidic pH (8.0). The work also describes using printed devices that can be loaded using multipipettes, and printed flexible, foldable channels in paper over areas larger than 100 cm 2 . This paper describes the use of a modified desktop plotter to fabricate simple patterns of hydrophilic microchannels in paper. We defined the boundaries of the microchannels by printing a solution of poly(dimethyl siloxane) (PDMS) in hexanes onto filter paper, using an x,y-plotter as a print-engine. Because PDMS is an elastomer, the paper could be bent and folded, without destroying the integrity of the channels. The channels have a minimum width of 1 mm, and the minimum spacing between two channels can be as small as 1 mm. These dimensions are large relative to those normally encountered in microfluidic systems, but they are the right size for the basic analytical and diagnostic devices that are our primary objectives, since readout of these devices will often involve visual observations of unmagnified spots of analytes. 1 We believe that this low-cost system for printing hydrophobic polymers and other materials on paper will be useful for prototyping simple paper-based diagnostics assays.A variety of simple diagnostic tests (for example, "dip-stick" tests) rely on paper-based assays. [2][3][4][5][6][7][8][9][10][11][12][13][14][15] Some of these tests analyze environmental conditions, 16,17 or detect illness (often in the developing world). [18][19][20] We have recently patterned paper into hydrophilic regions demarcated by hydrophobic walls using photolithography and conventional photoresists (SU-8; and poly(methyl methacrylate), or PMMA). 1 The hydrophilic regions act as microfluidic channels, in which capillary action wicks aqueous samples into the device. This design provides the basis for diagnostic systems in paper that are more complex than the simplest dip-stick assays (in the sense that multiple assays can be performed on a small array with ~5-20 μL of blood, urine, or other fluid), but simpler and more affordable than highertechnology microfluidic assays. 20 An advantage of using PDMS to pattern the p...

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“…In recent years, colloidal gold has been introduced into immunochemistry and the immunochromatographic strip assay has been a popular rapid diagnostic tool for detecting hormones (HCG), viruses (HIV, hepatitis B and C), bacteria, parasite antigens and β-agonists (Millipore, 1999;Verheijen et al, 1998;Zhang et al, 2006). These assays depend on a detector reagent which shifts to a special ligand immobilized on the surfaces of the membrane (Millipore, 1999;Birnbaum et al, 1992;Paek et al, 2000).…”

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confidence: 99%

Development of an immunochromatographic assay for rapid detection of 1‐Aminohydantoin in urine specimens

Liu

et al. 2008

Biomedical Chromatography

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A rapid immunochromatographic assay was developed and validated for detection of 1-aminohydantoin (AHD) in urine specimens. Colloidal gold-labeled polyclonal antibody specific to AHD derivative was used as the marker; based on the competitive reactivity theory, the metabolite of nitrofurantoin after derivatization with benzaldehyde would compete with carboxyphenyl AHD derivative-conjugated ovalbumin. The test strip could efficaciously detect the novel analyte with a visual detection limit of 10 ng mL(-1) and high specificity. The reliability of the assay was determined by testing 80 standard samples comparing with enzyme-linked immunosorbent assay. The semi-quantitative detection was accomplished in less than 15 min with low cost, especially for requirements of rapid and simple screening. This is the first publication of an immunochromatographic assay for detection of nitrofuran residues.

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Development of an immunochromatographic lateral flow test strip for detection of β-adrenergic agonist Clenbuterol residues

Cited by 140 publications

References 18 publications

Lateral flow (immuno)assay: its strengths, weaknesses, opportunities and threats. A literature survey

Lateral flow (immuno)assay: its strengths, weaknesses, opportunities and threats. A literature survey

Low-Cost Printing of Poly(dimethylsiloxane) Barriers To Define Microchannels in Paper

Development of an immunochromatographic assay for rapid detection of 1‐Aminohydantoin in urine specimens

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