Development of an immunochromatographic strip for the rapid detection of 10 β-agonists based on an ultrasensitive monoclonal antibody

Abstract

A sensitive monoclonal antibody, 3G10, derived from a mouse immunized with an immunogen of salbutamol hapten conjugated to keyhole limpet hemocyanin was developed against β-agonists, with a view to establishing a rapid gold nanoparticle immunochromatographic strip. The antibody displayed a 50% inhibitory concentration (IC 50) of 1.04 ng/mL for salbutamol and simultaneously detected nine other β-agonists (clenbuterol, cimatero, brombuterol, mabuterol, terbutaline, clenpenterol, carbuterol, mapenterol, pirbuterol) with uniform cross-reactivity. The immunochromatographic strip had cutoff values of 5 ng/mL for salbutamol and clenbuterol, 20 ng/mL for cimaterol, brombuterol and mabuterol, and 50 ng/mL for terbutaline, clenpenterol, carbuterol, mapenterol and pirbuterol. The collective advantages of the newly developed strip, such as high affinity, sensitivity and rapidity, support its utility in detecting β-agonists from actual biological samples, such as urine.

Effect of dietary supplementation with hyperimmunized hen egg yolk powder on diarrhoea incidence and intestinal health of weaned pigs

“…To solve these post-weaning problems, antibiotics have been widely used in the animal feed industry as growth promoters and a medicated feed additive to reduce susceptibility to infectious disease (Vondruskova et al, 2010). Unfortunately, widespread use or misuse of antibiotics has resulted in increasing multiple antimicrobial resistance and antibiotic residue in edible animal products (Sun et al, 2018;Liu et al, 2017;Han et al, 2016). Hence, safe, natural and effective products have been studied extensively as alternatives to antibiotic feed additives.…”

Ultrasensitive immunochromatographic strip for detection of cyproheptadine

“…If the control line was darker while the test line was lighter, or had no color, the sample was positive. If both control and test lines appeared dark red, the sample was considered to be negative (Liu et al, 2017). The detection line could be judged by the elimination of the line value.…”

Development of an immunochromatographic strip for the rapid detection of maduramicin in chicken and egg samples

“…The GNPs solution was uniform wine red in appearance. The solution was cooled to room temperature and stored at 4°C for further use (Chen et al, 2017;Kong et al, 2017;Liu et al, 2017).…”