Ultrasensitive immunochromatographic strip for detection of cyproheptadine

Guo, Mengyuan; Sun, Li; Liu, Liqiang; Song, Shanshan; Kuang, Hua; Cui, Gang

doi:10.1080/09540105.2018.1490395

Cited by 13 publications

(11 citation statements)

References 20 publications

(20 reference statements)

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“…Spiked sample study confirmed that the QBs-ICA was capable of being used as portable and rapid tools for on-site testing. Under optimal conditions, the developed QBs-ICA had visual detection limit of 0.625 ng/mL, the sensitivity and speedy were much improved compared with the reported instrumental analysis and immunoassays (Fente et al, 2009;Yang et al, 2015;Guo et al, 2018).…”

Section: Discussionmentioning

confidence: 92%

“…As shown in Table S1, the highest IR was 61%, while 1.0 mg/mL of the Hapten-OVA were immobilized on the test line, and the QBs-mAb probe was 1 µL, respectively. (Yang et al, 2015) LC-MC/MC pig urine 0.48 >60 ND a (Fente et al, 2009) GC-ICA pig urine ND a 15 5 (Guo et al, 2018) QBs-ICA pig urine, pork 0 In addition, for the stability and sensitivity optimization of QBs-ICA, an immunological dynamic analysis was conducted to optimize the interpretation time and pH value (Huang et al, 2013). The kinetic curves were established by recording FI T , FI C , every 1 min for 30 min to calculate FI T /FI C ratio.…”

Section: Optimization Of Qbs-icamentioning

confidence: 99%

“…Based on the resulting mAb, highly luminescent QBs were introduced in ICA as signal-amplification probes for CYP detection. This study provided a portable, user friendly, on-site detection approach which showed better sensitivity and speedy than the reported instrumental analysis and immunoassays (Fente et al, 2009;Yang et al, 2015;Guo et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

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Sensitive Immunochromatographic Assay Using Highly Luminescent Quantum Dot Nanobeads as Tracer for the Detection of Cyproheptadine Hydrochloride in Animal-Derived Food

Li¹,

Yang²,

Liu³

et al. 2020

Front. Chem.

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Cyproheptadine hydrochloride (CYP), used as human and veterinary drug, has been used illegally as feed additive for food-producing animals, which could remain in food and jeopardize human health. There is a need for on-site detection of CYP residue in animal-derived food. In this study, a hapten was designed, and a specific monoclonal antibody (mAb) was developed to detect CYP with an IC 50 of 1.38 ng/mL and negligible cross-reactivity (CR) for other analogs. Forthermore, a high sensitive immunochromatographic assay (QBs-ICA) was developed using quantum dot nanobeads as reporters. The assay showed the linear detection range (IC 20-IC 80) of 0.03-0.52 ng/mL, the limit of detection (LOD) and visual detection limit (VDL) reached to 0.01 and 0.625 ng/mL, respectively. Spiked recovery study in pig urine and pork confirmed that the QBs-ICA was applicable for on-site testing. This assay showed better sensitivity and speedy than the reported instrumental analysis and immunoassays.

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Section: Discussionmentioning

confidence: 92%

Section: Optimization Of Qbs-icamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Sensitive Immunochromatographic Assay Using Highly Luminescent Quantum Dot Nanobeads as Tracer for the Detection of Cyproheptadine Hydrochloride in Animal-Derived Food

Li¹,

Yang²,

Liu³

et al. 2020

Front. Chem.

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“…We determined the optimum coating antigen and mAb concentrations in ic-ELISA by a checkerboard assay (M. Guo et al, 2018). Coating antigen was diluted to 0.03, 0.1, 0.3, and 1 μg/mL, and mAb was diluted to 0.03, 0.1, 0.3, and 1 μg/mL.…”

Section: Development Of Ic-elisamentioning

confidence: 99%

Rapid detection of praziquantel using monoclonal antibody-based ic-ELISA and immunochromatographic strips

Shen

Liu

et al. 2019

Food and Agricultural Immunology

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Praziquantel (PZQ) is a broad-spectrum antiparasitic drug used in mammals and fish. In this study, we developed an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) for the detection of PZQ residues. The hapten PZQ-HS produced by introducing an amino group at a benzene ring synthesized the immunogen and the coating antigen with carrier proteins. Highly sensitive monoclonal antibodies (mAbs) were prepared following mouse immunization and cell fusion. Under optimum conditions, the half maximal inhibitory concentration of the cell line 4E9 was 7.4 ng/mL, and the working range was 2.4-22.8 ng/mL. Immunochromatographic strips (ICS) were developed for the rapid detection of PZQ residues in mackerel. The reliability of the ICS assay was verified with PZQ-negative mackerel and confirmed by HPLC/MS. The cutoff value for ICS was 50 ng/mL PZQ. Our findings revealed that ic-ELISA and ICS can be used for the rapid detection of PZQ in mackerel.

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“…Each mouse was injected with incomplete Freund's adjuvant mixed with immunogen (50 μg per mice). After the third immunization, tail blood samples were collected 6-8 days after each booster and examined by indirect competitive ELISA (ic-ELISA) (Guo et al, 2018). Here, we selected the mouse with the highest inhibitory rate and titre and then gave it a final immunization with 25 μg of immunogen through intraperitoneal injection on day 21.…”

Section: Monoclonal Antibody Preparationmentioning

confidence: 99%

Development of an immunochromatographic strip assay based on a monoclonal antibody for detection of cimaterol

Liu

Cui

et al. 2019

Food and Agricultural Immunology

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An anti-cimaterol (cima) monoclonal antibody, 2H8, was prepared and used to develop an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and an immunochromatographic test strip assay for the detection of cima, a newly described βagonist. The half-maximal inhibitory concentration of the antibody was 1.56 ng/mL, and no cross-reactivity with other β-adrenergic agonists was observed. The antibody's limit of detection was 0.416 ng/mL, and its linear range of detection was 0.416-5.868 ng/mL. The recovery rates of cima in swine urine ranged from 86% to 92.5%, and the test strip had a cutoff value of 10 ng/mL. Both ic-ELISA and the developed strip assay are effective when detecting cima in swine urine.

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Ultrasensitive immunochromatographic strip for detection of cyproheptadine

Cited by 13 publications

References 20 publications

Sensitive Immunochromatographic Assay Using Highly Luminescent Quantum Dot Nanobeads as Tracer for the Detection of Cyproheptadine Hydrochloride in Animal-Derived Food

Sensitive Immunochromatographic Assay Using Highly Luminescent Quantum Dot Nanobeads as Tracer for the Detection of Cyproheptadine Hydrochloride in Animal-Derived Food

Rapid detection of praziquantel using monoclonal antibody-based ic-ELISA and immunochromatographic strips

Development of an immunochromatographic strip assay based on a monoclonal antibody for detection of cimaterol

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