Development of an immunochromatographic strip assay based on a monoclonal antibody for detection of cimaterol

Abstract

An anti-cimaterol (cima) monoclonal antibody, 2H8, was prepared and used to develop an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and an immunochromatographic test strip assay for the detection of cima, a newly described βagonist. The half-maximal inhibitory concentration of the antibody was 1.56 ng/mL, and no cross-reactivity with other β-adrenergic agonists was observed. The antibody's limit of detection was 0.416 ng/mL, and its linear range of detection was 0.416-5.868 ng/mL. The recovery rates of cima in swine urine ranged from 86% to 92.5%, and the test strip had a cutoff value of 10 ng/mL. Both ic-ELISA and the developed strip assay are effective when detecting cima in swine urine.

Development of monoclonal antibodies for the detection of diuron in water and sugarcane and their application in immunochromatographic strips

“…At the same time, the NC membrane had the test and control lines drawn with the coating antigen, and the goat anti-mouse IgG was attached to the center of the card. 30–32…”

Determination of 18 β-Agonists in Aquatic Products Using High-Performance Liquid Chromatography Tandem Mass Spectrometry

“…The methods for the determination of various β-agonist residues include enzyme-linked immunoassay (ELISA), , strip test or colorimetric assay, gas chromatography tandem mass spectrometry (GC-MS/MS), and liquid chromatography tandem mass spectrometry (LC-MS/MS), which were validated in milk and the muscle tissue of cattle, swine, goat, etc. , ELISA and the strip test are low cost, simple, and rapid but have poor accuracy and sensitivity. Furthermore, detailed compound information cannot be confirmed.…”

Rapid detection of rifampicin in fish using immunochromatographic strips

“…For these reasons, immunoassays are becoming an increasingly popular alternative to detect analytes because they are relatively easy, low cost, sensitive, and can screen large numbers of samples (Shen et al, 2019;Song et al, 2019;Wang et al, 2019;Xu et al, 2019). Lateral flow immunochromatographic assays (LFIA) are a type of the immunoassay method whereby all reagents needed for the assay are sprayed onto the strip and they have several advantages over the above methods such as speed (results can be obtained within 5-10 min), specificity, simplicity, and sensitivity (Guo et al, 2019;Li et al, 2020b;Liu et al, 2019;Wang et al, 2020). To our knowledge, this is the first study to attempt to detect RFP in fish samples using a LFIA.…”