Development of An Immunogenomic Landscape For The Competing Endogenous RNAs Network of Peri-Implantitis

Abstract

Background: Peri-implantitis is an inflammation that occurs around the implant, resulting in varying degrees of inflammatory damage to the soft and hard tissues. The characteristic criterion is the loss of the supporting bone in an inflammatory environment. However, the specific mechanisms and biomarkers involved in peri-implantitis remain to be further studied. Recently, competing endogenous RNAs (ceRNA) and immune microenvironment have been found to play a more important role in the inflammatory process. In our study, we analyzed the expression of immune related microRNAs (miRNAs), long noncoding RNAs (lncRNAs) and message RNAs (mRNAs) in peri-implantitis by analyzing GSE33774 and GSE57631.Methods: In this study, we explored the expression profile data of immune-related lncRNAs, miRNAs and mRNAs, and constructed immune-related ceRNA network involved in the pathogenesis of peri-implantitis. In addition, the CIBERSORT was used to evaluate the content of immune cells in normal tissues and peri-implantitis to detect the immune microenvironment of peri-implantitis.Results: In the analysis, the corresponding lncRNAs, 14 DElncRNAs, 16 DEmiRNAs, and 18 DEmRNAs were used to establish an immune related ceRNA network constructed and the immune infiltration patterns associated with peri-implantitis was discovered. Through the mutual verification of the two datasets, we found that GSK3B and miR-1297 may have important significance in the immune microenvironment and pathogenesis of peri-implantitis and GSK3B was closely related to four types of immune cells, which needs to be further proved by later experiments. Conclusions: Through immune-related ceRNA network, IRGs and immune cell infiltration can further comprehensively understand the pathogenesis of peri-implantitis, which built up an immunogenomic landscape with clinical significance for peri-implantitis.One-sentences summary: GSK3B and miR-1297 may have important significance in the immune microenvironment and pathogenesis of peri-implantitis.

Identification of Potential Hub Genes ,Immune Cells and Target Drugs of Peri-Implantitis related to chromosome Using Bioinformatics Tool

“…Due to the rapid expansion of transcriptome studies, microarray analysis research is spreading quickly, which is leading to an increased understanding of the molecular mechanisms underlying oral diseases [21,22] . We can discover important genes that can be used as biomarkers for the diagnosis and prognosis of peri-impantitis by the integration of multiple related datasets, and we can determine the association between important genes and immune cells.…”

CCK regulates osteogenic differentiation through TNFα/NF-κB in peri-implantitis

“…23,24 In this study of peri-implantitis, microarray transcriptome analysis was used to analyze changes in the gene expression profile of patients with peri-implantitis. 16,17 In this study, we performed mRNA sequencing of the GEO database GSE106090. Compared with the healthy group, 262 genes were upregulated and 215 were downregulated in the periodontal tissues of patients with peri-implantitis.…”

“…Microarray transcriptome analysis is valuable for studying changes in the gene expression profiles of patients with peri-implantitis. 16,17 Herein, we used these methods to study the molecular mechanisms underlying peri-implantitis. We analyzed differentially expressed genes in the periodontal tissues of patients with peri-implantitis and those of healthy subjects using the Gene Expression Omnibus (GEO) database GSE106090.…”