Development of an Immunosensor Based on Surface Plasmon Resonance for Simultaneous Residue Analysis of Three Pesticides —Boscalid, Clothianidin, and Nitenpyram— in Vegetables

Abstract

A simultaneous immunosensor based on surface plasmon resonance (SPR) was developed for determination of 3 pesticides -boscalid, clothianidin and nitenpyram- instead of the direct competitive enzyme-linked immunosorbent assays (dcELISAs) widely used as individual determination methods. Carboxy groups that introduced compounds to their pesticides were designed, and conjugates of them and bovine serum albumin were immobilized onto separate channels of the same sensor chip. When a mixture of 3 monoclonal antibodies reacted to each pesticide, and 3 pesticides were injected into the SPR immunosensor, each channel showed specific reactivity at 15 - 93 ng mL for boscalid, 6.7 - 27 ng mL for clothianidin, and 7.3 - 62 ng mL for nitenpyram. Recovery tests using vegetables spiked with a mixture of 3 pesticides showed good results: 75 - 90%, 88 - 104%, and 72 - 105%, respectively, with a high correlation to results of the dcELISAs. The SPR immunosensor would be useful for the determination of pesticide residues in vegetables.

Simultaneous Detection of Six Different Types of Pesticides by an Immunosensor Based on Surface Plasmon Resonance

“…2, called haptens in this study, and synthesized as described previously. 10,12,[19][20][21][22] All of the chemicals were collected for safe disposal discard after use. Anti-azoxystrobin monoclonal antibody (MoAb), anti-chlorfenapyr MoAb, anti-imazalil MoAb, anti-isoxathion MoAb, and anti-nitenpyram MoAb were provided from Horiba Ltd. (Kyoto, Japan).…”

“…Immunoassays for pesticide analysis often react not only with the target pesticide but also with the structurally related pesticides. 19 These cross-reactions would be mainly derived from the similarity among the examined pesticide structures. When simultaneous detection of several pesticides is examined, the cross-reactivities would bring difficulty to the data analysis.…”

“…1A. 19 This SPR immunosensor was useful for detecting these pesticides, but it could not detect more than three pesticides, even though such numbers of pesticides are usually applied to crops.…”

Efficient Preservation of Acetylcholinesterase at Room Temperature for Facile Detection of Organophosphorus Pesticide

“…Enzymes are used in many fields including clinical diagnosis, food quality control and environmental monitoring. [1][2][3][4][5][6] Acetylcholinesterase (AChE) is an enzyme related to cognitive and neuromuscular processes.…”

Biosensors Using an Antibody as a Recognition Element

“…Hirayama et al reported an immunosensor based on surface plasmon resonance to detect three pesticide residues (boscalid, clothianidin and nitenpyram) in vegetables simultaneously. 6 They designed three channels modified with different pesticides, and evaluated the availability of the developed sensor. Yin et al reported on aptamer-based colorimetric sensors for Ochratoxin A.…”