Development of an in vivo model of laryngeal burn injury

Dion, Gregory R.; Teng, Stephanie; Bing, Renjie; Hiwatashi, Nao; Amin, Milan R.; Branski, Ryan C.

doi:10.1002/lary.26123

Cited by 11 publications

(14 citation statements)

References 21 publications

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“…The current study was approved by the Institutional Animal Care and Use Committee (IACUC) at the New York University School of Medicine. As part of two consecutive airway studies, 22 female New Zealand white rabbits (2.7–3.4 kg) underwent tubeless tracheotomy, as described by Boon et al and our group . In each animal, an open stoma was created to allow airflow to the lungs, permitting laryngeal interventions while preserving ventilation and oxygenation.…”

Section: Methodsmentioning

confidence: 99%

“…As part of two consecutive airway studies, 22 female New Zealand white rabbits (2.7-3.4 kg) underwent tubeless tracheotomy, as described by Boon et al and our group. 4,7 In each animal, an open stoma was created to allow airflow to the lungs, permitting laryngeal interventions while preserving ventilation and oxygenation. To create these stomas, the anterior portion of five or six tracheal rings was removed, and the tracheal mucosa was approximated to the skin edges using polyglycolic acid suture to create a permanent stoma.…”

Section: Methodsmentioning

confidence: 99%

“…The authors reported that all six rabbits in the study survived, but that a respiratory emergency occurred despite an intensive monitoring program to maintain the tracheostoma . In prior work from our laboratory, this same tubeless tracheostoma was utilized for an airway study . The purpose of the current study was to review our experience with rabbit tracheotomy, outline challenges with the procedure, and document associated morbidity and mortality for researchers to consider when developing future protocols.…”

Section: Introductionmentioning

confidence: 99%

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Morbidity and mortality associated with preclinical tracheostomy models

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Morbidity and mortality associated with preclinical tracheostomy models

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“…This may suggest that the model animals in the present experiment would not develop subglottic stenosis in the cricoid cartilage where this usually observed, because the totally normal tissues appeared at week two after the injury. Previously, in a rabbit model established to study inhalational injury in the larynx [7], the researcher found that elevated experimental temperature was followed by increased severity of injury or depth in injured tissues. In the present study, 100℃ saturated steam was used to induce injury in the larynx, and it could be inferred that when given a larger quantity of heat by extending the time of inducing injury or using other methods to induce injury, the cricoid cartilage should be observed to have severer damage, which is possibly deep to the cartilage or muscle lamina.…”

Section: Histologic Demonstrations Related To Laryngeal Stenosismentioning

confidence: 99%

“…In another study conducted by Zhang [6], which aimed to examine the pathological changes of these three types using a canine model, three dogs were observed to die of acute laryngeal stenosis caused by oedema. Gregory R et al [7] also reported a rabbit model, in which vocal fold obstruction was directly observed from endoscopic imaging at 24 hours after inhalational injury. However, to date, all animal model studies on inhalational injury have focused on acute laryngeal stenosis.…”

Section: Introductionmentioning

confidence: 99%

Pathological changes of larynx in inhalational thermal injury causing laryngeal stenosis based on a canine model

Wang

Liu

Wan

et al. 2020

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Objective This study aimed to explore the endoscopic and histologic feature of the larynx in inhalational thermal injury and its correlation with delayed laryngeal stenosis. Methods A total of 18 healthy, male adult Beagle dogs were randomly divided into six groups: group C (control group), and groups 1–5. All experimental dogs inhaled 100℃ saturated steam for five seconds to induce laryngeal injury. Endoscopic and histologic examinations were conducted immediately (group 1), and at two weeks (group 2), four weeks (group 3), six weeks (group 4) and eight weeks (group 5) after injury. Endoscopic morphologies of the larynx and histologic morphologies of epiglottis, glottis and cricoid cartilage were observed and analyzed. Results Granulation tissue in larynx was observed in group 2, and this shrank to 2/3 and 1/3 of the size in groups 3 and 4, respectively. Microscopically, declined severity of epithelial exfoliations in different regions and patial repair in the glottis were observed. In the epiglottis, cartilage cells appeared degenerative and necrotic and had partial repair. Muscle fibers and cartilage cells were severely injured and had no repair in the glottis, but in the cricoid cartilage, they were totally normal. Conclusion The granulation tissue formation, uncompletely repaired tissues in the larynx found after inhalational thermal injury may indicate the process of laryngeal stenosis. The cricoid cartilage that appeared slight pathology in the present study may due to the thermal absorption and protective effect of the upper parts.

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Effect of continuous local dexamethasone on tissue biomechanics and histology after inhalational burn in a preclinical model

Malka,

Gonzales,

Detar

et al. 2023

Laryngoscope Investig Oto

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ObjectiveInhalational burns frequently lead to dysphonia and airway stenosis. We hypothesize local dexamethasone delivery via a novel drug‐eluting electrospun polymer‐mesh endotracheal tube (ETT) reduces biomechanical and histologic changes in the vocal folds in inhalational burn.MethodsDexamethasone‐loaded polymer mesh was electrospun onto ETTs trimmed to transglottic endolaryngeal segments and secured in nine Yorkshire Crossbreed swine with directed 150°C inhalation burns. Uncoated ETTs were implanted in nine additional swine with identical burns. ETT segments were maintained for 3 and 7 days. Vocal fold (VF) structural stiffness was measured using automated‐indentation mapping and compared across groups and to four uninjured controls, and matched histologic assessment performed. Statistical analysis was conducted using two‐way ANOVA with Tukey's post hoc test and Wilcoxon rank‐sum test.ResultsVF stiffness after burn decreased with longer intubation, from 19.4 (7.6) mN/mm at 3 days to 11.3 (5.2) mN/mm at 7 days (p < .0001). Stiffness similarly decreased with local dexamethasone, from 25.9 (17.2) mN/mm at 3 days to 18.1 (13.0) mN/mm at 7 days (p < .0001). VF stiffness in the dexamethasone group was increased compared to tissues without local dexamethasone (p = .0002), and all groups with ETT placement had higher tissue stiffness at 3 days (p < .001). No significant change in histologic evidence of epithelial ulceration or fibrosis was noted, while an increased degree of inflammation was noted in the dexamethasone group (p = .04).ConclusionLocal dexamethasone delivery increases VF stiffness and degree of inflammation compared to uncoated ETTs in an acute laryngeal burn model, reflected in early biomechanical and histologic changes in an inhalational burn model.

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Development of an in vivo model of laryngeal burn injury

Abstract: NA Laryngoscope, 127:186-190, 2017.

Cited by 11 publications

References 21 publications

Morbidity and mortality associated with preclinical tracheostomy models

Morbidity and mortality associated with preclinical tracheostomy models

Pathological changes of larynx in inhalational thermal injury causing laryngeal stenosis based on a canine model

Effect of continuous local dexamethasone on tissue biomechanics and histology after inhalational burn in a preclinical model

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