Development of an indirect competitive immunochromatographic strip test for rapid detection and determination of anticancer drug, harringtonine

Sakamoto, Seiichi; Yusakul, Gorawit; Nuntawong, Poomraphie; Kitisripanya, Tharita; Putalun, Waraporn; Miyamoto, Tomofumi; Tanaka, Hiroyuki; Morimoto, Satoshi

doi:10.1016/j.jchromb.2017.01.032

Cited by 21 publications

(20 citation statements)

References 12 publications

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“…Sensitivity is a key performance factor for developments in analytical methods, especially when the target compound is highly toxic with a trace amount of exposure. When an antibody was applied for both ICA and icELISA, the ICA procedures can sometimes produce lower sensitivity than icELISA . Enhancing sensitivity is one of the challenges for ICA development.…”

Section: Resultsmentioning

confidence: 99%

“…When an antibody was applied for both ICA and icELISA, the ICA procedures can sometimes produce lower sensitivity than icELISA. [14][15][16] Enhancing sensitivity is one of the challenges for ICA development. Recently, external antibody formats have been developed for ICA, where the primary antibody reacts with target compounds before ICA testing.…”

Section: Sensitivitymentioning

confidence: 99%

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Preincubation format for a sensitive immunochromatographic assay for monocrotaline, a toxic pyrrolizidine alkaloid

Yusakul

Sakamoto

Chanpokapaiboon

et al. 2019

Phytochemical Analysis

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Introduction Monocrotaline (MCT), which is classified as a 1,2‐dehydropyrrolizidine alkaloid (DHPA), is a toxic compound that is mainly produced by Crotalaria spp. MCT contamination in cereals and herbs leads to hepatitis, gastroenteritis, pulmonary vasculitis and hypertension, and different types of cancer. The current analytical methods for MCT are complicated and expensive using liquid chromatography equipped with mass spectrometry detection. Objective The aim of this study was to develop a simple and sensitive preincubation format for an immunochromatographic assay (PI‐ICA) for MCT detection. Methodology We conducted the PI‐ICA via incubation of an MCT‐containing sample with an anti‐MCT monoclonal antibody conjugated with colloidal gold before strip dipping. We compared the PI‐ICA detection sensitivity with that of the conventional ICA (Conv‐ICA) format. Results The PI‐ICA was sensitive with a limit of detection (LOD) of 0.61 ng/mL, which is a 16‐fold improvement over the Conv‐ICA format. These results indicated that the PI‐ICA method exhibits high binding specificity for MCT and low cross‐reactivity towards retronecine, retrorsine, senecionine and heliotrine. Sample solutions from plants containing MCT and related DHPAs produced positive results via PI‐ICA analysis. Conclusions The proposed PI‐ICA system provides a highly sensitive method compared to Conv‐ICA. In addition, the developed PI‐ICA method is simple and highly effective for detecting MCT contamination.

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Section: Resultsmentioning

confidence: 99%

Section: Sensitivitymentioning

confidence: 99%

Preincubation format for a sensitive immunochromatographic assay for monocrotaline, a toxic pyrrolizidine alkaloid

Yusakul

Sakamoto

Chanpokapaiboon

et al. 2019

Phytochemical Analysis

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“…The antibody-colloidal gold nanoparticle conjugates were used as the biosensors for the developed LFA and were prepared via a previously reported protocol with some modifications. 17 Briefly, the environment of the colloidal gold nanoparticles (1 mL) was adjusted to be weakly alkaline (i.e., pH = 9.0) using a potassium carbonate buffer solution (20 μL). Then, MAb E8 (50 μg) was mixed with the pH-adjusted colloidal gold suspension, and the mixture was left on an orbital shaker at 50 rpm for 10 min at room temperature.…”

Section: Mab E8-colloidal Gold Nanoparticle Conjugationmentioning

confidence: 99%

The colloidal gold nanoparticle‐based lateral flow immunoassay for fast and simple detection of plant‐derived doping agent, higenamine

Nuntawong

Ochi

Chaingam

et al. 2020

Drug Testing and Analysis

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Higenamine (HM), an alkaloid found in various plant species, is obtained when norcoclaurine synthase selectively condenses dopamine and 4-hydroxypheny lacetaldehyde to give (S)-higenamine ((S)-HM). The World Anti-doping Agency has listed HM as a prohibited agent in athletics. As a result, many commercial, academic, and regulatory bodies across the globe are invested in finding a rapid method for (S)-HM detection. In the current study, a lateral flow immunoassay (LFA) was developed in which the relevant biosensor was generated as a conjugate of the monoclonal antibody against (S)-HM (namely, MAb E8) and colloidal gold nanoparticles. The HM-γ-globulin conjugates and rabbit anti-mouse IgG antibodies were placed in the test and control zones, respectively. The free (S)-HM molecules in the samples and the immobilized HM-γ-globulin conjugates competitively reacted with the developed biosensor in the LFA. An inverse relationship existed between the biosensors' visible response, which was noted by the variation in the intensity of a pinkish spot in the test zone, and the content of the free (S)-HM. The limit of detection of the developed LFA was 156 ng/mL. Various validation methods confirmed that the LFA exhibited sufficient sensitivity, selectivity, repeatability, and reliability, making it ideal for (S)-HM detection in plant samples and plant-containing products. The developed system required only a small sample volume (20 μL) and a concise sample preparation time compared with conventional LFAs. Thus, the LFA reported in this study could serve as a rapid response kit for the detection of (S)-HM in plant samples. K E Y W O R D S colloidal gold nanoparticles, higenamine, immunochromatographic test strips, lateral flow immunoassay, monoclonal antibody 1 | INTRODUCTION Higenamine (HM; 1-[(4-hydroxyphenyl)methyl]-1,2,3,4-tetrahydroiso quinoline-6,7-diol, Figure 1) is a benzyl tetrahydroisoquinoline alkaloid derived from various plant species, including Aconitum carmichaelii Debeaux. (Ranunculaceae), Nandina domestica Thunb., (Berberidaceae), Nelumbo nucifera Gaertn. (Nelumbonaceae), and Asarum heterotrophies F. Schmidt (Aristolochiaceae). 1,2 Given its chiral character, studies have revealed that the norcoclaurine synthase conformational selectively condenses dopamine and 4-hydroxyphenylacetaldehyde to form the specific enantiomer, (S)-HM. 3-5 From a pharmacological perspective, HM is a partial

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“…The analysis can be completed in 20 min without sophistic equipment. The method has been widely used to detect multiple antigens for instance hormones, antibodies in serum ( Zhao et al., 2017 ), viruses ( Sun et al., 2018 ), bacteria ( Wen-de et al., 2017 ), parasites ( Zhuo et al., 2017 ), biotin ( Yao et al., 2017 ), microelement ( Fu et al., 2017 ), and drug residue ( Sakamoto et al., 2017 ). In this study, a CGIS based on monoclonal antibodies against R. anatipestifer outer membrane protein A ( OmpA ) was developed to rapidly detect R. anatipestifer in ducks.…”

Section: Introductionmentioning

confidence: 99%

Development of a colloidal gold immunochromatographic strip for rapid detection of Riemerella anatipestifer in ducks

et al. 2020

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Riemerella anatipestifer is one of the major bacterial pathogens of ducks and causes significant economic losses in poultry agriculture. Usually, methods for detecting R. anatipestifer infection need specialized equipment and highly skilled personnel. In this study, a novel colloidal gold immunochromatographic strip was developed for rapid detection of R. anatipestifer in ducks. The monoclonal antibodies 2D5 and 2A6 against R. anatipestifer were used as colloidal gold-labeled protein and capture protein, respectively, to recognize the bacteria in tryptic soy broth medium culture and in hearts of infected ducks. The goat anti-mouse IgG antibody was labeled on nitrocellulose membrane as a control for C line. The labeling pH was optimized as 10.0, and the concentration of 2D5 labeled to colloidal gold particles was optimized as 18 μg/mL. The strip specifically detected serotypes 1, 2, and 10 R. anatipestifer strains and showed no cross-reaction with Escherichia coli , Salmonella enterica, and Pasteurella multocida strains. The sensitivity of the strip for detecting R. anatipestifer was 1.0 × 10 6 colony forming unit. The strips remained stable for up to 8 mo at 4°C, and the detection can be completed within 15 min. The strip can detect R. anatipestifer in hearts of the ducks experimentally infected with R. anatipestifer but not infected with E. coli , which were also confirmed with bacterial isolation followed by multiplex polymerase chain reaction. These results suggested that the strips are reliable methods for identification of R. anatipestifer in laboratories and in duck farms.

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Development of an indirect competitive immunochromatographic strip test for rapid detection and determination of anticancer drug, harringtonine

Cited by 21 publications

References 12 publications

Preincubation format for a sensitive immunochromatographic assay for monocrotaline, a toxic pyrrolizidine alkaloid

Preincubation format for a sensitive immunochromatographic assay for monocrotaline, a toxic pyrrolizidine alkaloid

The colloidal gold nanoparticle‐based lateral flow immunoassay for fast and simple detection of plant‐derived doping agent, higenamine

Development of a colloidal gold immunochromatographic strip for rapid detection of Riemerella anatipestifer in ducks

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