Development of an on-line weak-cation exchange liquid chromatography–tandem mass spectrometric method for screening aldehyde products in biological matrices

Eggink, Mark; Charret, Ségolène; Lingeman, H.; Kool, Jeroen; Niessen, W.M.A.; Irth, H.

doi:10.1016/j.jchromb.2009.09.043

Cited by 20 publications

(29 citation statements)

References 35 publications

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“…The aliphatic aldehyde derivatives form a strong molecular ion during positive ESI-MS and produce two prominent ions due to the loss of 59 and 87 Da as outlined in the Figure 18. 4-HHE and 4-HNE derivatives have the additional loss of 18 Da due to the loss of water, and the most characteristic product ions for those aldehydes are formed as a result of the loss of 77 Da and 105 Da [[53]]. This aniline-based reagent approach was further developed by the creation of 4-APEBA (4-(2-((4-bromophenethyl)dimethylammonio)ethoxy)benzenaminium dibromide) reagent [[54]].…”

Section: Fatty Aldehyde Analysis By Lc/msmentioning

confidence: 99%

Mass spectrometry of fatty aldehydes

Berdyshev

2011

Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biolog

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Fatty aldehydes are important components of the cellular lipidome. Significant interest has been developed towards the analysis of the short chain α,β-unsaturated and hydroxylated aldehydes formed as a result of oxidation of polyunsaturated fatty acids. Multiple gas chromatography-mass spectrometry (GC/MS) and subsequently liquid chromatography-mass spectrometry (LC/MS) approaches have been developed to identify and quantify short-chain as well as long-chain fatty aldehydes. Due to the ability to non-enzymaticaly form Schiff bases with amino groups of proteins, lipids, and with DNA guanidine, free aldehydes are viewed as a marker or metric of fatty acid oxidation and not the part of intracellular signaling pathways which has significantly limited the overall attention this group of molecules have received. This review provides an overview of current GC/MS and LC/MS approaches of fatty aldehyde analysis as well as discusses technical challenges standing in the way of free fatty aldehyde quantitation.

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Section: Fatty Aldehyde Analysis By Lc/msmentioning

confidence: 99%

Mass spectrometry of fatty aldehydes

Berdyshev

2011

Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biolog

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“…Thus this methodology allows the determination of ultratrace MDA concentrations in plants and makes it possible to find subtle and significant differences in MDA levels when plants are exposed to thermal stress. The detection and quantitation limits reached were comparable and inclusive better than the ones reported for others methodologies based on mass spectrometry detection [ 15 , 16 , 21 , 25 ], as well as the methodologies that used the same matrix [ 13 ] or those that quantified MDA without derivatization [ 24 , 26 ].…”

Section: Introductionmentioning

confidence: 53%

“…Thus analytical approaches using hydrazine-based derivatization reagents have been reported. These strategies were coupled to separation techniques such as liquid or gas chromatography employing a wide variety of detection systems, such as UV [ 14 ], tandem mass spectrometry (MS/MS) [ 15 , 16 ], and fluorescence [ 17 ], among others. However, one of the most serious drawbacks related to the use of hydrazine is that the derivatives have to be extracted, dried, and reconstituted before analysis and these multiple time consuming steps reduce its applicability to a large amount of samples.…”

Section: Introductionmentioning

confidence: 99%

Development of a Novel, Sensitive, Selective, and Fast Methodology to Determine Malondialdehyde in Leaves of Melon Plants by Ultra-High-Performance Liquid Chromatography-Tandem Mass Spectrometry

Yonny

Torressi

Nazareno

et al. 2017

Journal of Analytical Methods in Chemistry

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Early production of melon plant (Cucumis melo) is carried out using tunnels structures, where extreme temperatures lead to high reactive oxygen species production and, hence, oxidative stress. Malondialdehyde (MDA) is a recognized biomarker of the advanced oxidative status in a biological system. Thus a reliable, sensitive, simple, selective, and rapid separative strategy based on ultra-high-performance liquid chromatography coupled to positive electrospray-tandem mass spectrometry (UPLC-(+)ESI-MS/MS) was developed for the first time to measure MDA, without derivatization, in leaves of melon plants exposed to stress conditions. The detection and quantitation limits were 0.02 μg·L−1 and 0.08 μg·L−1, respectively, which was demonstrated to be better than the methodologies currently reported in the literature. The accuracy values were between 96% and 104%. The precision intraday and interday values were 2.7% and 3.8%, respectively. The optimized methodology was applied to monitoring of changes in MDA levels between control and exposed to thermal stress conditions melon leaves samples. Important preliminary conclusions were obtained. Besides, a comparison between MDA levels in melon leaves quantified by the proposed method and the traditional thiobarbituric acid reactive species (TBARS) approach was undertaken. The MDA determination by TBARS could lead to unrealistic conclusions regarding the oxidative stress status in plants.

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“…Fresh juice and its water-soluble fraction blocked the entry of extracellular calcium through calcium channels and inhibited the release of intracellularly stored calcium in the vascular smooth muscle cell [14,15].…”

Section: Cynomorium Coccineummentioning

confidence: 99%

“…Many medicinal plants have demonstrated to exert antidiabetic and hypotensive activities in different animal models as well as in vitro and a lot of compounds responsible for this pharmacological activity have been isolated [13][14][15][16]. This non-exhaustive short review focuses on some medicinal plants and botanical compounds with hypoglycemic and hypotensive activities…”

Section: Introductionmentioning

confidence: 99%