Development of an oral biosensor for salivary amylase using a monodispersed silver for signal amplification

Smart electrochemical biosensors: From advanced materials to ultrasensitive devices

“…The MED platform was used to detect cisplatin, an anticancer drug with parts per trillion detection limit [2]. The concept has also been successfully tested for antigen-antibody interactions [23] and more recently a modified MED concept was explored for the detection of base pair mismatches in microcystis DNA [26].…”

“…The amylase MED-based biosensor design, characterization and optimization followed similar procedure as reported earlier [23,54]. Instead of using pristine amylase or histatin protein samples, the sensor was used to detect similar proteins in human salivary samples.…”

“…Our group is exploring the possibility of building functional polymeric structures through electrochemical and mechanical synthesis and photopolymerization [23][24][25][26][27]. Metal nanoparticles dispersed in polymeric matrices have recently been the subject of intense studies aimed at developing nanocomposite films [28][29][30][31][32][33].…”

“…An amperometric immunosensor for monitoring the level of protein amylase in human saliva was developed in our laboratories by Aluoch et al [23]. The sensor comprised of the salivary antigen monolayer self-assembled onto gold electrode via cystamine sulfur bonds.…”

Electrochemical immunoassay of estrone at an antibody-modified conducting polymer electrode towards immunobiosensors

“…The key of electrochemical immunosensor is the immobilization of immunological sensitive compounds on the electrode, and different strategies have been used to immobilize immunoactive compounds on different electrodes [10][11][12][13]. Among all the conventional immobilization methods, electrogeneration of organic conducting polymers is one of the few methods that allow reproducible functionalization of the conductive surface, of complex geometry, with a precise spatial resolution [14].…”