Development of an ultra fast liquid chromatography–tandem mass spectrometry method for simultaneous determination of cefazedone and etimicin in beagle dog plasma: Application to the pharmacokinetic study of the combination of cefazedone and etimicin injections

Cui, Yan; Ma, Ning; Li, Xiaoyan; Li, Mingjie; Li, Minghua; Lee, Song; Liu, Mingxia; Li, Qing; Bi, Kaishun

doi:10.1016/j.jchromb.2014.10.018

Cited by 6 publications

(3 citation statements)

References 17 publications

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“…In LC-MS, spectinomycin forms strong solvent adducts with water or methanol [105,109]. HFPA as an IPR was also used in clinical studies to determine tobramycin [56], amikacin [58], etimicin [117] in serum or plasma.…”

Section: Fragmentation Of Aminoglycosides In Ms/msmentioning

confidence: 99%

Challenges in the determination of aminoglycoside antibiotics, a review

Farouk

Azzazy

Niessen

2015

Analytica Chimica Acta

122

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Section: Fragmentation Of Aminoglycosides In Ms/msmentioning

confidence: 99%

Challenges in the determination of aminoglycoside antibiotics, a review

Farouk

Azzazy

Niessen

2015

Analytica Chimica Acta

122

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“…Several bioanalytical methods have been developed to determine the etimicin concentration in different biological matrices of humans and animals by resonance Rayleigh scattering (Wang et al, 2010) or liquid chromatography-tandem mass spectrometry (LC-MS/MS) (Cui et al, 2014;Yao et al, 2017;Sun et al, 2022). Resonance Rayleigh scattering is a complex and time-consuming spectral method (Wang et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

“…Resonance Rayleigh scattering is a complex and time-consuming spectral method (Wang et al, 2010). As for the LC-MS/MS methods developed for preclinical studies and therapeutic drug monitoring, some used ion-pairing reagents in the mobile phase, leading to limit the lower limit of quantification (Cui et al, 2014); some adopted the antibiotics commonly used in clinical practice as the internal standard, limiting its application in human clinical studies (Yao et al, 2017); some was not with ideal chromatography retention and peak shape for etimicin (Sun et al, 2022). Besides, the quantitative ranges of etimicin have not been sufficiently wide in published LC-MS/MS methods.…”

Section: Introductionmentioning

confidence: 99%

Development and validation of two bioanalysis methods for the determination of etimicin in human serum and urine by liquid chromatography-tandem mass spectrometry: Applications to a human pharmacokinetic and breakpoint study

et al. 2023

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Etimicin is a fourth-generation aminoglycoside antibiotic. It has potent activity and low toxicity when employed for the treatment of Gram-negative and Gram-positive bacterial infections. The pharmacokinetics of etimicin in humans have not been elucidated completely. Two liquid chromatography-tandem mass spectrometry (LC-MS/MS) bioanalytical methods, without the use of any ion-pairing reagents, were developed and validated for the quantification of etimicin in human samples of serum and urine. Using a deuterated reagent as the internal standard, analytes in serum and urine samples were extracted by protein precipitation and dilution before LC-MS/MS analysis, respectively. For the two methods, chromatographic separations were undertaken under isocratic elution of water–ammonia solution–acetic acid (96:3.6:0.2, v/v/v) and methanol at 50%:50% and a flow rate of 0.35 ml/min within 5 min. A Waters XTerra MS C18 column (2.1 × 150 mm, 3.5 μm) and a column temperature of 40°C were chosen. A Sciex Qtrap 5500 mass spectrometer equipped with an electrospray ion source was used in both methods under multiple-reaction monitoring in positive-ion mode. The two methods showed good linearity, accuracy, and precision with high recovery and a minimal matrix effect in the range of 50.0–20000 ng/ml for serum samples and 50.0–10000 ng/ml for urine samples, respectively. Carry-over effects were not observed. Etimicin remained stable in human samples of serum or urine under the storage, preparation, and analytical conditions of the two methods. These two simple and reliable methods were applied successfully to a dose-escalation, phase I clinical trial of etimicin in Chinese healthy volunteers after intravenous administration of single and multiple doses. Based on these two methods we ascertained, for the first time, the comprehensive pharmacokinetics of etimicin in humans, which will be used for the exploration of the breakpoint research further.

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Design of fluorescence system based on rutin functionalized gold nanoparticles: Sensitive detection of etimicin via a smartphone in the food and human samples

Liú

Zhao

et al. 2022

Arabian Journal of Chemistry

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Development of an ultra fast liquid chromatography–tandem mass spectrometry method for simultaneous determination of cefazedone and etimicin in beagle dog plasma: Application to the pharmacokinetic study of the combination of cefazedone and etimicin injections

Cited by 6 publications

References 17 publications

Challenges in the determination of aminoglycoside antibiotics, a review

Challenges in the determination of aminoglycoside antibiotics, a review

Development and validation of two bioanalysis methods for the determination of etimicin in human serum and urine by liquid chromatography-tandem mass spectrometry: Applications to a human pharmacokinetic and breakpoint study

Design of fluorescence system based on rutin functionalized gold nanoparticles: Sensitive detection of etimicin via a smartphone in the food and human samples

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