Development of an Ultrahigh-Performance Liquid Chromatography–Electrospray Ionization–Tandem Mass Spectrometry Method for the Simultaneous Determination of Salicylic Acid, Jasmonic Acid, and Abscisic Acid in Rose Leaves

Abstract

This paper describes a method to detect and quantitate the endogenous plant hormones (±)-2-cis-4-trans-abscisic acid, (−)-jasmonic acid, and salicylic acid by means of ultrahigh-performance liquid chromatography−tandem mass spectrometry (UHPLC−MS/MS) in hybrid rose leaf matrices. Deuterium-labeled [ 2 H 6 ] (+)-2-cis-4-trans-abscisic acid, [ 2 H 6 ] (±)-jasmonic acid, and [ 2 H 4 ]-salicylic acid were used as internal standards. Rose samples (10 mg) were extracted with methanol/water/acetic acid (10:89:1) and subsequently purified on an Oasis MCX 1 cm 3 Vac SPE cartridge. Performance characteristics were validated according to Commission Decision 2002/657/EC. Recovery, repeatability, and within-laboratory reproducibility were acceptable for all phytohormones tested at three different concentrations. The decision limit and detection capability for (±)-2-cis-4-trans-abscisic acid, (−)-jasmonic acid, and salicylic acid were 0.0075 and 0.015 μg/g, 0.00015 and 0.00030 μg/g, and 0.0089 and 0.018 μg/g, respectively. Matrix effects (signal suppression or enhancement) appeared to be high for all substances considered, implying the need for quantitation based on matrix-matched calibration curves.

Rapid method for quantification of anthocyanidins and anthocyanins in human biological samples

“…The LC-MS/MS method was linear for all analytes over the concentration range (10-500 ng mL -1 for Cy, Dp, Pg, Pt; and 2-100 ng mL -1 for Mv, Pn, Cy3Glu, Cy3Gal, Cy3Ara, Dp3Gal, Mv3Gal, Pg3Glu, Pt3Glu) studied in both plasma and urine. The resultant r 2 values for all anthocyanidins and anthocyanins were close to 1 (Table 1), which indicated good linearity within the considered concentration ranges (Bosco, Daeseleire, Van Pamel, Scariot, & Leus, 2014). The LOD and LOQ values of all anthocyanidin and anthocyanin analytes were calculated in blank plasma and urine to determine the sensitivity of the method.…”

A Selective Joint Determination of Salicylic Acid in Actinidia chinensis Combining a Molecularly Imprinted Monolithic Column and a Graphene Oxide Modified Electrode

“…Then, 1.0 g of the homogenized sample was immersed in a 10.0-mL mixture of methanol-water-acetic acid (10:89:1, v/v/v) for 16 h at 4 C in the dark to perform extraction. 24 After 10 min of centrifugation at 4000 rpm, the supernatant was filtered through a 0.22-μm membrane (Xingya Scavenging Material Company, Shanghai, China). Then, the filtrate was rotary evaporated to yield a residue that was subsequently reconstituted in 250 mL ultrapure water and stored at 4 C prior to analysis.…”

Multiple Reaction Monitoring Mode Based Liquid Chromatography-Mass Spectrometry Method for Simultaneous Quantification of Brassinolide and Other Plant Hormones Involved in Abiotic Stresses

“…The measurement of endogenous hormonal levels in plants has received a great deal of interest owing to their potential role in signaling networks and molecular mechanisms. Consequently, several analytical methods have been adapted to measure plant hormones, including enzyme-linked immunosorbent assay (ELISA), high-performance liquid chromatography with diode-array and fluorometric detection (HPLC-DAD/FLD), liquid chromatography-mass spectrometry (LC-MS), and gas chromatography-mass spectrometry (GC-MS) [ 10 , 11 ]. Among these, mass spectrometry (MS) methods have been found to provide high sensitivity and selectivity for analysis of plant hormones [ 12 ].…”

“…Among these, mass spectrometry (MS) methods have been found to provide high sensitivity and selectivity for analysis of plant hormones [ 12 ]. GC-MS is routinely applied for the simultaneous analysis of various hormones in plant tissues; however, it is relatively time consuming when compared to LC-MS [ 11 ]. Although several LC-MS methods have been developed for simultaneous analysis of multiple plant hormones [ 11 – 15 ], there is no any single suitable LC-MS method for simultaneous analysis of BR and other major hormones involved in drought stress.…”

“…GC-MS is routinely applied for the simultaneous analysis of various hormones in plant tissues; however, it is relatively time consuming when compared to LC-MS [ 11 ]. Although several LC-MS methods have been developed for simultaneous analysis of multiple plant hormones [ 11 – 15 ], there is no any single suitable LC-MS method for simultaneous analysis of BR and other major hormones involved in drought stress. The probable reason behind this could be the concentration of BR or high matrix effect caused by other coeluting plant hormones.…”