Multiple Reaction Monitoring Mode Based Liquid Chromatography-Mass Spectrometry Method for Simultaneous Quantification of Brassinolide and Other Plant Hormones Involved in Abiotic Stresses

Abstract

Plant hormones are the key regulators of adaptive stress response. Abiotic stresses such as drought and salt are known to affect the growth and productivity of plants. It is well known that the levels of plant hormones such as zeatin (ZA), abscisic acid (ABA), salicylic acid (SA), jasmonic acid (JA), and brassinolide (BR) fluctuate upon abiotic stress exposure. At present, there is not any single suitable liquid chromatography-mass spectrometry (LC-MS) method for simultaneous analysis of BR and other plant hormones involved in abiotic stresses. In the present study, we developed a simple, sensitive, and rapid method for simultaneous analysis of five major plant hormones, ZA, ABA, JA, SA, and BR, which are directly or indirectly involved in drought and salt stresses. The optimized extraction procedure was simple and easy to use for simultaneous measurement of these plant hormones in Arabidopsis thaliana. The developed method is highly reproducible and can be adapted for simultaneous measurement of changes in plant hormones (ZA, ABA, JA, SA, and BR) in response to abiotic stresses in plants like A. thaliana and tomato.

Endophytic Trichoderma citrinoviride isolated from mountain-cultivated ginseng (Panax ginseng) has great potential as a biocontrol agent against ginseng pathogens

“…Ginseng hormones, zeatin (ZA), abscisic acid (ABA), and jasmonic acid (JA), were analyzed as previously described [24], [25]. Approximately 50 mg of frozen samples was extracted with 980 μL methanol:acetic acid (99:1, v/v) and 20 μL internal standard solution.…”

Transcriptome Analysis and Identification of Genes Associated with Floral Transition and Flower Development in Sugar Apple (Annona squamosa L.)

“…The exogenous IAA contents were determined using a FOCUS GC-DSQII (Thermo Fisher Scientific Inc., Austin, TX, USA; Shen et al, 2014). Additionally, ABA and Cytokinin (ZRs) were detected using a UFLC-MS/MS system as described Kasote et al (2016). …”

Characterization and Quantification of Polyphenols and Triterpenoids in Thinned Young Fruits of Ten Pear Varieties by UPLC-Q TRAP-MS/MS

“…In recent years, ultra-performance liquid chromatography coupled with triple quadrupole mass spectrometry (UPLC-MS/MS) has been widely and successfully used for characterizing and quantifying many compounds in complex samples [10,11]. Especially, through use of the multiple reaction monitoring (MRM) scan mode, MS/MS detection could achieve high sensitivity and selectivity without establishing baseline separation of the target analytes, and a large array of compounds could be simultaneously determined in a very short time [12]. In addition, precursor and production ion monitoring could not only increase the specificity of detection but also aid in identifying the molecules.…”