Development of an underivatized LC-MS/MS method for quantitation of 14 neurotransmitters in rat hippocampus, plasma and urine: Application to CUMS induced depression rats

Han, Xuemei; Qin, Yanjie; Zhu, Ying; Zhang, Xinlin; Wang, Nan-Xi; Rang, Ying; Zhai, Xuejia; Lu, Yongning

doi:10.1016/j.jpba.2019.06.043

Cited by 32 publications

(15 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the following GC-MS-based non-targeted plasma metabolomic analysis, we found the level of 21 metabolites significantly altered in the OVX group compared with the sham group, among which the levels of two metabolites were increased and those of 18 metabolites like L-tyrosine and L-tryptophan were reduced. The results of reduced levels of L-tyrosine and L-tryptophan in this depressive animal model were in accordance with the results of CUMS depressive rats (Han et al, 2019), or the results of OVX-induced menopause (Lee et al, 2016). After XS volatile oil administration, L-tyrosine and L-tryptophan levels were upregulated in OVX rats.…”

Section: Discussionsupporting

confidence: 88%

Integrated Network Pharmacology and GC-MS–Based Metabolomics to Investigate the Effect of Xiang-Su Volatile Oil Against Menopausal Depression

Yang

Chen

et al. 2021

Front. Pharmacol.

View full text Add to dashboard Cite

Menopausal depression perplexes a great number of women in later life. Xiangfu-Zisu (Xiang-Su), a traditional Chinese herbal pair composed of rhizomes of Cyperus rotundus L. (Xiangfu) and leaves of Perilla frutescens (L.) Britt. (Zisu), is frequently reported with antidepressant-like effects. The volatile oil from Xiangfu and Zisu has shown good antidepressant action, but its mechanism is still unclear. This study aimed to investigate the pharmacological mechanism of Xiang-Su (XS) volatile oil against menopausal depression through gas chromatography–mass spectrometry (GC-MS)-based network pharmacology and metabolomics. First, ADME screening was performed on actual detected components of XS volatile oil to obtain active constituents, and then duplicates of active constituent–related targets and menopausal depression–related targets were collected. These duplicates were considered as targets for XS volatile oil against menopausal depression, followed by GO and KEGG enrichment analyses. It showed that a total of 64 compounds were identified in XS volatile oil, and 38 active compounds were screened out. 42 overlapping genes between 144 compound-related genes and 780 menopausal depression–related genes were obtained. Results showed that targets of SLC6A4 and SLC6A3, regulation of serotonergic and dopaminergic synapses, were involved in the antidepressant mechanism of XS volatile oil. Next, antidepressant-like effect of XS volatile oil was validated in menopausal rats by ovariectomy (OVX) combined with chronic unpredictable mild stress (CUMS). Behavioral tests, biochemical analysis, and GC-MS–based non-targeted plasma metabolomics were employed to validate the antidepressant effect of XS volatile oil. Experimental evidence demonstrated that XS volatile oil reversed behavioral parameters in the sucrose preference test (SPT), open-field test (OFT), forced swim test (FST), and serum estradiol levels in OVX rats. Furthermore, results of metabolomics indicated that XS volatile oil mainly acts on regulating metabolic pathways of phenylalanine, tyrosine and tryptophan biosynthesis, tyrosine metabolism, and tryptophan metabolism, which were corresponding with the above-predicted results. These data suggest that network pharmacology combined with metabolomics provides deep insight into the antidepressant effect of XS volatile oil, which includes regulating key targets like SLC6A4 and SLC6A3, and pathways of serotonergic and dopaminergic synapses.

show abstract

Section: Discussionsupporting

confidence: 88%

Integrated Network Pharmacology and GC-MS–Based Metabolomics to Investigate the Effect of Xiang-Su Volatile Oil Against Menopausal Depression

Yang

Chen

et al. 2021

Front. Pharmacol.

View full text Add to dashboard Cite

show abstract

“…Phenylalanine metabolism was also found to be associated with depression in a depressive rat model (Xu et al, 2019;Yang et al, 2020a). Imbalance of central and peripheral phenylalanine metabolism was found in rats subjected to chronic unpredictable mild stress (Han et al, 2019). Plasma phenylalanine levels showed an association with the Diagnostic and Statistical Manual of Mental Disorders, Third Edition depression subgroup (dysthymic disorders, major recurrent depression, and bipolar depression) (Chiaroni et al, 1990).…”

Section: Discussionmentioning

confidence: 96%

“…Ke et al(Ke et al, 2019) identi ed tyrosine as a consistent biomarker associated with PSD. An animal study also showed that tyrosine is associated with the development of depression(Han et al, 2019). Changes in central and peripheral tyrosine and phenylalanine concentrations are thought to be related to the pathogenesis of depression(Ogawa et al, 2018).…”

mentioning

confidence: 99%

Peripheral tissues metabolites and biological functions in Post-stroke Depression

Liu¹,

Pu²,

Zhou³

et al. 2021

Preprint

View full text Add to dashboard Cite

Post-stroke depression (PSD) is the most common and severe neuropsychiatric complication after stroke. However, the molecular mechanism of PSD is still unclear. Previous studies have identified peripheral tissues metabolites associated with PSD using metabolomics techniques. We searched and systematically summarized metabolites that may be involved in metabolic changes in peripheral tissues of patients with PSD from the Metabolite Network of Depression Database (MENDA) and other biomedical databases. MetaboAnalyst5.0 software was used for pathway analysis and enrichment analysis of differential metabolites, and subgroup analyses were performed according to tissue types and metabolomics techniques. We identified 47 metabolites that were differentially expressed between patients with and without PSD. Five differential metabolites were found in both plasma and urine, including L-glutamic acid, pyroglutamic acid, palmitic acid, L-phenylalanine, and L-tyrosine. We integrated these metabolites into metabolic pathways, and six pathways were significantly altered. These pathways could be roughly divided into three modules including amino acid metabolism, nucleotide metabolism, and glucose metabolism. Among them, the most significantly altered pathway was “phenylalanine metabolism” and the pathway containing the most associated molecules was “aminoacyl-tRNA biosynthesis”, which deserve further study to elucidate their role in the molecular mechanism of PSD. In summary, metabolic changes in peripheral tissues are associated with PSD, especially the disruption of “phenylalanine metabolism” and “aminoacyl-tRNA biosynthesis” pathways. This study provides clues to the metabolic characteristics of patients with PSD, which may help to elucidate the molecular pathogenesis of PSD.

show abstract

“…Consistent with previous studies, in our present work, we found that the levels of leucine and valine were decreased in the brain tissues of the CORT group, while ACE treatment significantly reversed these effects. Moreover, tyrosine, as an aromatic amino acid, is a precursor for the synthesis of catecholamine neurotransmitters ( 51 , 52 ). It was reported that tyrosine was essential in neurotransmitters synthesis under the catalysis of tyrosine hydroxylase ( 53 , 54 ).…”

Section: Discussionmentioning

confidence: 99%

Metabolomics Analysis on Mice With Depression Ameliorated by Acupoint Catgut Embedding

et al. 2021

View full text Add to dashboard Cite

Depression is a prevalent mental disease characterized by persistent low mood, lack of pleasure, and exhaustion. Acupoint catgut embedding (ACE) is a kind of modern acupuncture treatment, which has been widely used for the treatment of a variety of neuropsychiatric diseases. To investigate the effects and underlying mechanism of ACE on depression, in this study, we applied ACE treatment at the Baihui (GV20) and Dazhui (GV14) acupoints of corticosterone (CORT)-induced depression model mice. The results showed that ACE treatment significantly attenuated the behavioral deficits of depression model mice in the open field test (OFT), elevated-plus-maze test (EPMT), tail suspension test (TST), and forced swimming test (FST). Moreover, ACE treatment reduced the serum level of adreno-cortico-tropic-hormone (ACTH), enhanced the serum levels of 5-hydroxytryptamine (5-HT), and noradrenaline (NE). Furthermore, metabolomics analysis revealed that 23 differential metabolites in the brain of depression model mice were regulated by ACE treatment for its protective effect. These findings suggested that ACE treatment ameliorated depression-related manifestations in mice with depression through the attenuation of metabolic dysfunction in brain.

show abstract

Development of an underivatized LC-MS/MS method for quantitation of 14 neurotransmitters in rat hippocampus, plasma and urine: Application to CUMS induced depression rats

Cited by 32 publications

References 26 publications

Integrated Network Pharmacology and GC-MS–Based Metabolomics to Investigate the Effect of Xiang-Su Volatile Oil Against Menopausal Depression

Integrated Network Pharmacology and GC-MS–Based Metabolomics to Investigate the Effect of Xiang-Su Volatile Oil Against Menopausal Depression

Peripheral tissues metabolites and biological functions in Post-stroke Depression

Metabolomics Analysis on Mice With Depression Ameliorated by Acupoint Catgut Embedding

Contact Info

Product

Resources

About