Development of anti-bovine IgA single chain variable fragment and its application in diagnosis of foot-and-mouth disease

Sridevi, N.; Shukra, A. M.; Neelakantam, B.; Anilkumar, J.; Madhanmohan, M.; Rajan, Sharmila; Chandran, Dev; Srinivasan, V.A.

doi:10.1556/eujmi.4.2014.1.3

Cited by 3 publications

(3 citation statements)

References 34 publications

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“…Superior serotype specificity was also obtained in a sandwich ELISA using recombinant integrin αvβ6 as a capture ligand and serotype‐specific monoclonal antibodies as detecting reagents (Ferris et al., ). Recombinant single‐chain variable fragment has also been developed as an alternative to immunoglobulins and applied as a detection agent in an ELISA (Sridevi et al., ).…”

Section: Introductionmentioning

confidence: 99%

Global Foot-and-Mouth Disease Research Update and Gap Analysis: 4 - Diagnostics

Knight-Jones

Robinson

Charleston

et al. 2016

Transbound Emerg Dis

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SummaryThis study assessed knowledge gaps in foot-and-mouth disease (FMD) research in the field of diagnostics. The study took the form of a literature review combined with research updates collected in 2014 from 33 institutes from around the world. Findings were used to identify priority areas for future FMD research.Molecular and genetic technologies, including sequencing, are developing at an increasing rate both in terms of capability and affordability. These advances potentiate progress in many other fields of research, from vaccine development to epidemiology. The development of RT-LAMP represents an important breakthrough allowing greater use and access to molecular diagnostics. It is now possible to determine virus serotype using PCR, although only for certain virus pools, continued progress is needed to cover the global spectrum of FMD viruses. Progress has also been made in the development of pen-side rapid diagnostics, some with the ability to determine serotype. However, further advances in pen-side serotype or strain determination would benefit both FMD-free countries and endemic countries with limited access to wellresourced laboratories.Novel sampling methods that show promise include air sampling and baited ropes, the latter may aid sampling in wildlife and swine. Studies of infrared thermography for the early detection of FMD have not been encouraging, although investigations are ongoing. Multiplex tests have been developed that are able to simultaneously screen for multiple pathogens with similar clinical signs. Crucial for assessing FMDV freedom, tests exist to detect animals that have been infected with FMDV regardless of vaccination status; however, limitations exist, particularly when testing previously vaccinated animals. Novel vaccines are being developed with complementary DIVA tests for this purpose. Research is also needed to improve the current imprecise approaches to FMD vaccine matching.The development of simple, affordable tests increases access to FMD diagnostics, greatly benefiting regions with limited laboratory capacity.

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Section: Introductionmentioning

confidence: 99%

Global Foot-and-Mouth Disease Research Update and Gap Analysis: 4 - Diagnostics

Knight-Jones

Robinson

Charleston

et al. 2016

Transbound Emerg Dis

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“…Apart from mAbs, recombinant antibody fragments such as the single-chain variable fragments (scFvs) were also used as detection antibodies in sandwich ELISA to detect FMDV-specific IgA in salivary samples from vaccinated and infected cattle ( 63 ). In addition, the variable heavy chain antibody fragments (VHHs) from camels have been explored for FMD diagnostic applications ( 76 ).…”

Section: Serological Methodsmentioning

confidence: 99%

Advances in the Diagnosis of Foot-and-Mouth Disease

et al. 2020

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Foot-and-mouth disease (FMD) is a devastating livestock disease caused by foot-and-mouth disease virus (FMDV). Outbreaks of this disease in a country always result in conspicuous economic losses to livestock industry and subsequently lead to serious socioeconomic damages due to the immediate imposition of trade embargo. Rapid and accurate diagnoses are imperative to control this infectious virus. In the current review, enzyme-linked immunosorbent assay (ELISA)-based methods used in FMD diagnosis are extensively reviewed, particularly the sandwich, liquid-phase blocking, and solid-phase competition ELISA. The differentiation of infected animals from vaccinated animals using ELISA-based methods is also highlighted, in which the role of 3ABC polyprotein as a marker is reviewed intensively. Recently, more studies are focusing on the molecular diagnostic methods, which detect the viral nucleic acids based on reverse transcription-polymerase chain reaction (RT-PCR) and RT-loop-mediated isothermal amplification (RT-LAMP). These methods are generally more sensitive because of their ability to amplify a minute amount of the viral nucleic acids. In this digital era, the RT-PCR and RT-LAMP are progressing toward the mobile versions, aiming for on-site FMDV diagnosis. Apart from RT-PCR and RT-LAMP, another diagnostic assay specifically designed for on-site diagnosis is the lateral flow immunochromatographic test strips. These test strips have some distinct advantages over other diagnostic methods, whereby the assay often does not require the aid of an external device, which greatly lowers the cost per test. In addition, the on-site diagnostic test can be easily performed by untrained personnel including farmers, and the results can be obtained in a few minutes. Lastly, the use of FMDV diagnostic assays for progressive control of the disease is also discussed critically.

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“…The in-house kit showed higher sensitivity in comparison to the commercial kit. This scFv holds promise in the detection of foot-and-mouth disease virus specifi c IgA in salivary samples by IC-ELISA and offers a simple, novel, cheap and effi cient option for detection of IgA, which could replace the usage of commercial IgA due to better sensitivity, reagent stability and ease of production [55].…”

Section: Development Of An Assay For Bovine Igamentioning

confidence: 99%

Production of recombinant antibodies using bacteriophages

Shukra

Sridevi

Chandran

et al. 2014

European Journal of Microbiology and Immunology

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Recombinant antibody fragments such as Fab, scFv, diabodies, triabodies, single domain antibodies and minibodies have recently emerged as potential alternatives to monoclonal antibodies, which can be engineered using phage display technology. These antibodies match the strengths of conventionally produced monoclonal antibodies and offer advantages for the development of immunodiagnostic kits and assays. These fragments not only retain the specificity of the whole monoclonal antibodies but also easy to express and produce in prokaryotic expression system. Further, these antibody fragments are genetically stable, less expensive, easy to modify in response to viral mutations and safer than monoclonal antibodies for use in diagnostic and therapeutic applications. This review describes the potential of antibody fragments generated using phage display and their use as diagnostic reagents.

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Development of anti-bovine IgA single chain variable fragment and its application in diagnosis of foot-and-mouth disease

Cited by 3 publications

References 34 publications

Global Foot-and-Mouth Disease Research Update and Gap Analysis: 4 - Diagnostics

Global Foot-and-Mouth Disease Research Update and Gap Analysis: 4 - Diagnostics

Advances in the Diagnosis of Foot-and-Mouth Disease

Production of recombinant antibodies using bacteriophages

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